Ethanol-mediated Oxidative Changes in Blood Lipids and Proteins Are Reversed by Aspirin-like Drugs

Piña, Martha Zentella de; Sandoval-Montiel, Adrián; Serrano-Alessandri, Laura; Montalvo-Javé, Eduardo E.; Zentella‐Dehesa, Alejandro; Piña, Enrique

doi:10.1016/j.arcmed.2006.09.019

Cited by 9 publications

(10 citation statements)

References 26 publications

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“…Priming of HSP-72 expression installed in vivo on PBMCs by ASA administration reached a maximal effect 2 h after treatment and remained even after 5 h. This priming effect correlates with previous studies were in vivo ASA administration led to physiological protection against ethanol cytotoxicity, such as reduction of protein carbonylation in plasma and PBMCs, as well as in TBARS from PBMCs [13]. The kinetics of these transient effects in vivo could be related with the pharmacodynamics of acetylsalicylic acid [22].…”

Section: Discussionsupporting

confidence: 83%

“…Administration of ASA in vivo protects against oxidative damage and elicits other physiological benefits; however, the biochemical and molecular mechanisms involved in these effects are not completely understood [13], [14], [15]. Previous reports from our group have found that in vivo ASA treatment reverts some biochemical markers of oxidative damage [13], suggesting the activation of a general cytoprotective mechanism. Indomethacin treatment followed by heat shock in HeLa cells activates HSF1 and confers cytoprotection against lethal heat shock [7].…”

Section: Introductionmentioning

confidence: 89%

“…In addition, it has been shown that ASA (l0 µM) can generate H 2 O 2 in rat adipose tissue, reaching a final concentration of 1 µM (unpublished personal communication from Zentella de Piña M, (May 2009)). It has also been reported that orogastric administration of ASA provides cytoprotection of rat lymphocytes against oxidizing agents [13], reversing some indicators of oxidative stress such as thiobarbituric acid reactive substances and protein carbonylation. Here, we have shown that the orogastric administration of ASA (45 mg/kg of body weight) to rats can prime isolated peripheral blood mononuclear cells (PBMCs) for faster kinetics of Heat Shock induced HSP-72.…”

Section: Introductionmentioning

confidence: 96%

“…These inductive effects of NSAIDs have been associated to the activation of HSF1 [8], [9], [10], [11], [12]. Administration of ASA in vivo protects against oxidative damage and elicits other physiological benefits; however, the biochemical and molecular mechanisms involved in these effects are not completely understood [13], [14], [15]. Previous reports from our group have found that in vivo ASA treatment reverts some biochemical markers of oxidative damage [13], suggesting the activation of a general cytoprotective mechanism.…”

Section: Introductionmentioning

confidence: 99%

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HSP-72 Accelerated Expression in Mononuclear Cells Induced In Vivo by Acetyl Salicylic Acid Can Be Reproduced In Vitro when Combined with H2O2

et al. 2013

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BackgroundAmong NSAIDs acetyl salicylic acid remains as a valuable tool because of the variety of benefic prophylactic and therapeutic effects. Nevertheless, the molecular bases for these responses have not been complete understood. We explored the effect of acetyl salicylic acid on the heat shock response.ResultsPeripheral blood mononuclear cells from rats challenged with acetyl salicylic acid presented a faster kinetics of expression of HSP-72 messenger RNA and protein in response to in vitro heat shock. This effect reaches its maximum 2 h after treatment and disappeared after 5 h. On isolated peripheral blood mononuclear cells from untreated rats, incubation with acetyl salicylic acid was ineffective to produce priming, but this effect was mimicked when the cells were incubated with the combination of H2O2+ ASA.ConclusionsAdministration of acetyl salicylic acid to rats alters HSP-72 expression mechanism in a way that it becomes more efficient in response to in vitro heat shock. The fact that in vitro acetyl salicylic acid alone did not induce this priming effect implies that in vivo other signals are required. Priming could be reproduces in vitro with the combination of acetyl salicylic acid+H2O2.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

HSP-72 Accelerated Expression in Mononuclear Cells Induced In Vivo by Acetyl Salicylic Acid Can Be Reproduced In Vitro when Combined with H2O2

et al. 2013

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“…They were acclimatized to laboratory conditions before the test and fed ad libitum. They were fasted 16 hours prior to the treatment [13].…”

Section: Testsmentioning

confidence: 99%

Evaluation of Acetaminophen Effect on Oxidative Stressed Mice by Peroxide Hydrogen

Zoubair¹,

Azzahra²,

Hmimid³

et al. 2013

AJBR

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Acetaminophen (Paracetamol) is among the most commonly used analgesic and antipyretic drugs worldwide, it's often, but anomalously, classified as non-steroidal anti-inflammatory drugs (NSAIDs) in textbooks of pharmacology [1,2]. This study aims to evaluate if paracetamol has an antioxidant effect, relative to its analgesic antipyretic and weak anti-inflammatory activities, or it possesses a cytotoxic potential. Oxidative stress was induced by intraperetoneal injection of peroxide hydrogen (H 2 O 2 ), and then a comparative study is made concerning the activities of the antioxidant enzymes SOD, CAT, and GR as well as lipid peroxidation levels in liver. An increase in SOD, CAT, GR activity and lipid peroxidation in mice treated with H 2 O 2 accompanied by paracetamol; compared to the group treated by vitamin C + H 2 O 2 showed that acetaminophen doesn't show any antioxidant effect. Moreover this study has suggested that acetaminophen induced cytotoxicity in liver mediated by increased oxidative stress and altered redox metabolism. Keywords: acetaminophen (paracetamol), peroxide hydrogen, oxidative stressCite This Article: BENKHASSI Zoubair, LAHLOU Fatima Azzahra, HMIMID Fouzia, LOUTFI Mohammed, BENAJI Brahim, and BOURHIM Noureddine, "Evaluation of Acetaminophen Effect on Oxidative Stressed Mice by Peroxide Hydrogen

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Comparing the effects of two neurotoxins in cortical astrocytes obtained from rats of different ages: involvement of oxidative damage

Alarcón-Aguilar

González-Puertos

Luna–López

et al. 2012

J of Applied Toxicology

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Oxidative stress has been recognized as a potential mediator of cell death. Astrocytes play an active role in brain physiology responding to harmful stimuli by activating astrogliosis, which in turn has been associated either with survival or degenerative events. The characterization of the mechanistic actions exerted by different toxins in astrocytes is essential to understand the brain function and pathology. As age plays a critical role in degenerative processes, the aim of this study was to determine whether the administration of equimolar concentrations of two neurotoxins evoking different toxic patterns can induce differential effects on primary astrocytes obtained either from newborn or adult rats, with particular emphasis on those events linked to oxidative stress as a potential source of damage. Primary cortical astrocyte cultures derived from rat brains were exposed to 1-methyl-4-phenylpyridinium (MPP+) or beta-amyloid peptide (β-amyloid). Mitochondrial functionality and cell viability were determined as physiological parameters, whereas lipid and protein oxidation were used as markers of oxidative damage. The results of these experiments pointed towards a higher vulnerability to MPP + over β-amyloid, on most of the tested markers. Hence, in order to allow a comprehensive evaluation of astrocytic responses against MPP + intoxication, a third astrocyte group was included for dose-response experiments: astrocytes derived from aged rats. The present data indicate that the differences associated with age were mainly found in astrocytes exposed to MPP + (25 and 50 μM) at 1-h treatment. Results are discussed in terms of the differential mechanisms involved in each model.

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Ethanol-mediated Oxidative Changes in Blood Lipids and Proteins Are Reversed by Aspirin-like Drugs

Cited by 9 publications

References 26 publications

HSP-72 Accelerated Expression in Mononuclear Cells Induced In Vivo by Acetyl Salicylic Acid Can Be Reproduced In Vitro when Combined with H2O2

HSP-72 Accelerated Expression in Mononuclear Cells Induced In Vivo by Acetyl Salicylic Acid Can Be Reproduced In Vitro when Combined with H2O2

Evaluation of Acetaminophen Effect on Oxidative Stressed Mice by Peroxide Hydrogen

Comparing the effects of two neurotoxins in cortical astrocytes obtained from rats of different ages: involvement of oxidative damage

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