Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts

Lan, Shubin; Wu, Lin; Zhang, Delu; Hu, Chunxiang; Liu, Yongding

doi:10.1016/j.soilbio.2010.12.007

Cited by 58 publications

(43 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chl-a content was taken as a measure of photosynthetic biomass in our experiment, and Chl-a content determination was conducted according to the description of Lan et al (2011). For lichen and moss crusts, two subsamples were prepared respectively.…”

Section: Crust Biomassesmentioning

confidence: 99%

Analysis of environmental factors determining development and succession in biological soil crusts

Lan

Zhang

et al. 2015

Science of The Total Environment

Self Cite

View full text Add to dashboard Cite

Section: Crust Biomassesmentioning

confidence: 99%

Analysis of environmental factors determining development and succession in biological soil crusts

Lan

Zhang

et al. 2015

Science of The Total Environment

Self Cite

View full text Add to dashboard Cite

“…Total nitrogen content was determined using the Kjeldahl procedure (Bremner and Mulvaney 1982). Chlorophyll a was extracted in 10 mL 95 % ethanol at 4°C for 24 h and determined with a spectrophotometer (Chen et al 2006;Lan et al 2010).…”

Section: Physicochemical Characteristicsmentioning

confidence: 99%

Community succession of bacteria and eukaryotes in dune ecosystems of Gurbantünggüt Desert, Northwest China

Zhang

2014

Extremophiles

View full text Add to dashboard Cite

Pyrosequencing and quantitative polymerase chain reaction of small subunit rRNA genes were used to provide a comprehensive examination of bacterial, cyanobacterial, and eukaryotic communities in the biological soil crusts (BSCs) of Gurbantünggüt Desert sand dunes (China). Three succession stages were recognized based on the analyses of eukaryotic communities: a late succession stage of BSCs in a swale with eukaryotes mainly related to the Bryophyta clade, an initial succession stage in a slope with barely any eukaryotic phototrophic microorganisms detected, and an intermediate succession type detected from both the swale and slope BSCs dominated by the phylum Chlorophyta. Moreover, the cyanobacterial community dominated all of the BSCs (48.2-69.5 % of the total bacteria) and differed among the three succession stages: sequences related to Microcoleus steenstrupii and the genus Scytonema were abundant in the later succession stage, whereas both the initial and intermediate stages were dominated by Microcoleus vaginatus. Compared with swales, BSCs from slopes are exposed to a harsher environment, e.g., higher irradiance and lower water availability, and thus may be restricted from developing to a higher succession stage. Other disturbances such as wind and grazing may explain the different succession stages observed in swales or slopes. However, no clear differences were detected from non-phototrophic bacterial communities of the three succession stages, and sequences related to Alphaproteobacteria and Actinobacteria were most abundant in all the BSCs. The closest matches for the most frequent non-phototrophic bacterial genera were mainly derived from harsh environments, indicating the robustness of these genera.

show abstract

“…The Chl-a was extracted in acetone and determined spectrometrically according to the description of Lan et al [18].…”

Section: Photosynthetic Biomassmentioning

confidence: 99%

Assessing Level of Development and Successional Stages in Biological Soil Crusts with Biological Indicators

Lan

Zhang

et al. 2013

Microb Ecol

Self Cite

View full text Add to dashboard Cite

Biological soil crusts (BSCs) perform vital ecosystem services, but the difference in biological components or developmental level still affects the rate and type of these services. In order to differentiate crust successional stages in quantity and analyze the relationship between crust developmental level and successional stages, this work determined several biological indicators in a series of different developmental BSCs in the Shapotou region of China. The results showed that crust developmental level (level of development index) can be well indicated by crust biological indicators. Photosynthetic biomass was the most appropriate to differentiate crust successional stages, although both photosynthetic biomass and respiration intensity increased with the development and succession of BSCs. Based on of the different biological compositions, BSCs were quantificationally categorized into different successional stages including cyanobacterial crusts (lichen and moss coverages <20 %), lichen crusts (lichen coverage >20 % but moss coverage <20 %), semi-moss crusts (moss coverage >20 % but <75 %), and moss crusts (moss coverage >75 %). In addition, it was found that cyanobacterial and microalgal biomass first increased as cyanobacterial crusts formed, then decreased when lots of mosses emerged on the crust surface; however nitrogen-fixing cyanobacteria and heterotrophic microbes increased in the later developmental BSCs. The structural adjustment of biological components in the different developmental BSCs may reflect the requirement of crust survival and material transition.

show abstract

Ethanol outperforms multiple solvents in the extraction of chlorophyll-a from biological soil crusts

Cited by 58 publications

References 27 publications

Analysis of environmental factors determining development and succession in biological soil crusts

Analysis of environmental factors determining development and succession in biological soil crusts

Community succession of bacteria and eukaryotes in dune ecosystems of Gurbantünggüt Desert, Northwest China

Assessing Level of Development and Successional Stages in Biological Soil Crusts with Biological Indicators

Contact Info

Product

Resources

About