Ethanol production and fermentation characteristics of recombinant saccharomyces cerevisiae strains grown on starch

Bírol, Gülnur; Önsan, Z. İlsen; Kırdar, Betül; Oliver, Stephen G.

doi:10.1016/s0141-0229(97)00244-5

Cited by 60 publications

(42 citation statements)

References 12 publications

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“…As a result of a relatively small number of studies, all but one of which were conducted since 1995, significant progress has been made in the area of utilization of nonnative substrates by virtue of heterologous expression of saccharolytic enzymes. In the case of starch, the results of Birol et al (57) are encouraging not only with respect to the feasibility of starch utilization, but also the with respect to the overall feasibility of enabling utilization of nonnative substrates. Increased rates of growth and hydrolysis as well as use of insoluble substrates would appear to be logical objectives for future work on starch utilization.…”

Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 85%

“…Growth of S. cerevisiae on starch, under aerobic conditions in most cases, has been demonstrated in several studies (145,474,531,646). In a significant breakthrough, Birol et al (57) reported the utilization of 100 g of starch per liter with production of 44 g of ethanol per liter and 8 g of cells per liter. Although ethanol and cell yields on starch were similar to those on glucose, the specific growth rate was nearly 10-fold lower on starch.…”

Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 99%

See 1 more Smart Citation

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,045

2,868

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Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

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Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 85%

Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,045

2,868

View full text Add to dashboard Cite

show abstract

“…For example, growth of genetically engineered S. cerevisiae strains on starch, under aerobic conditions in most cases, has been demonstrated in several studies (De Moraes et al, 1995;Murai et al, 1997;Petersen et al, 1998;. In a significant breakthrough, Birol et al (1998) reported the utilization of 100 g of starch per liter with production of 44 g of ethanol per liter and 8 g of cells per liter. However, although ethanol yields on starch were similar to those on glucose, the specific growth rate was nearly 10-fold lower on starch.…”

Section: Introductionmentioning

confidence: 99%

Starch fermentation by recombinant saccharomyces cerevisiae strains expressing the α‐amylase and glucoamylase genes from lipomyces kononenkoae and saccharomycopsis fibuligera

Eksteen

Rensburg

Otero

et al. 2003

Biotech & Bioengineering

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Lipomyces kononenkoae and Saccharomycopsis fibuligera possess highly efficient alpha-amylase and/or glucoamylase activities that enable both of these yeasts to utilize raw starch as a carbon source. Eight constructs containing the L. kononenkoae alpha-amylase genes (LKA1 and LKA2), and the S. fibuligera alpha-amylase (SFA1) and glucoamylase (SFG1) genes were prepared. The first set of constructs comprised four single gene cassettes each containing one of the individual amylase coding sequences (LKA1, LKA2, SFA1 or SFG1) under the control of the phosphoglycerate kinase gene (PGK1) promoter and terminator, while the second set comprised two single cassettes containing SFA1 and SFG1 linked to their respective native promoters and terminators. The third set of constructs consisted of two double-gene cassettes, one containing LKA1 plus LKA2 under the control of the PGK1 promoter and terminator, and the other SFA1 plus SFG1 controlled by their respective native promoters and terminators. These constructs were transformed into a laboratory strain Saccharomyces cerevisiae (Sigma1278b). Southern-blot analysis confirmed the stable integration of the different gene constructs into the S. cerevisiae genome and plate assays revealed amylolytic activity. The strain expressing LKA1 and LKA2 resulted in the highest levels of alpha-amylase activity in liquid media. This strain was also the most efficient at starch utilization in batch fermentations, utilizing 80% of the available starch and producing 0.61g/100 mL of ethanol after 6 days of fermentation. The strain expressing SFG1 under the control of the PGK1 expression cassette gave the highest levels of glucoamylase activity. It was shown that the co-expression of these heterologous alpha-amylase and glucoamylase genes enhance starch degradation additively in S. cerevisiae. This study has resulted in progress towards laying the foundation for the possible development of efficient starch-degrading S. cerevisiae strains that could eventually be used in consolidated bioprocessing, and in the brewing, whisky, and biofuel industries.

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“…Recombinant yeasts which coproduce glucoamylase and ␣-amylase have meanwhile been developed to further improve the efficiency of starch fermentation (2,4,6,15,21,22). We also have used a cell surface engineering system based on ␣-agglutinin to demonstrate the advantages of yeast strains codisplaying amylolytic enzymes and have succeeded in producing ethanol from soluble and low-temperature-cooked corn starch using yeast strains which display Rhizopus oryzae glucoamylase and codisplay or secrete Bacillus stearothermophilus ␣-amylase (21,22).…”

mentioning

confidence: 99%

Direct Production of Ethanol from Raw Corn Starch via Fermentation by Use of a Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase and α-Amylase

Shigechi¹,

Koh²,

Fujita

et al. 2004

Appl Environ Microbiol

203

106

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Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis ␣-amylase by using the C-terminal-half region of ␣-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.

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Ethanol production and fermentation characteristics of recombinant saccharomyces cerevisiae strains grown on starch

Cited by 60 publications

References 12 publications

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Starch fermentation by recombinant saccharomyces cerevisiae strains expressing the α‐amylase and glucoamylase genes from lipomyces kononenkoae and saccharomycopsis fibuligera

Direct Production of Ethanol from Raw Corn Starch via Fermentation by Use of a Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase and α-Amylase

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