Ethanol production from green coconut fiber using a sequential steam explosion and alkaline pretreatment

Ribeiro, Vitor Troccoli; Campolina, Amanda Castro; Costa, Willyan Araújo da; Padilha, Carlos Eduardo de Araújo; Filho, José Daladiê Barreto da Costa; Leitão, Ana Laura Oliveira de Sá; Rocha, Juliene da Câmara; Santos, Everaldo Silvino dos

doi:10.1007/s13399-022-03100-0

Cited by 10 publications

(5 citation statements)

References 42 publications

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“…The raw fiber was determined to contain 3.51% of ashes, 25.04% of extractives, 33.96% of cellulose, 10.16% of hemicellulose, and 21.32% of lignin, all on a dry basis. The values are close to other studies that used green coconut fibers, such as [43] (38.0% holocellulose, 39.9% lignin), [3] (25.6% cellulose, 23.5% hemicellulose, 32.2% lignin), [10] (28.8% cellulose, 29.2% hemicellulose, 30.5% lignin), and [30] (27.7% cellulose, 15.5% hemicellulose, 32.6% lignin).…”

Section: Biomass Composition and Pretreatmentsupporting

confidence: 88%

“…The coconut powder was pretreated by a steam explosion following the methodology described by the work presented in [30]. Accordingly, 500.0 g of the biomass was inserted in the reactor (14 L, UpControl, Caxias do Sul, Brazil), and steam was injected until the temperature of 210 • C was reached.…”

Section: Steam Explosionmentioning

confidence: 99%

“…The mild conditions used (R 0 = 2.40) may have not been able to cause effective changes in the biomass composition. Ribeiro et al (2019) [30] studied the steam explosion of the green coconut fiber at three different severity factors (R 0 ), 2.17, 3.21, and 4.23. Again, mild conditions have not yielded significant changes in the biomass composition, being the highest R 0 the only one that significantly enriched the material in cellulose.…”

Section: Biomass Composition and Pretreatmentmentioning

confidence: 99%

“…The low production of the enzyme can be explained by the low cellulose content of the biomass, which is not enough to induce the cellulase expression, and by the The use of raw biomass yielded CMCase activity only on the sixth day of cultivation (0.77 ± 0.10 UI/g), and the maximum FPase activity was observed on the second day (0.15 ± 0.08 UI/g). The low production of the enzyme can be explained by the low cellulose content of the biomass, which is not enough to induce the cellulase expression, and by the presence of lignin, which strongly adsorbs the cellulases, reducing the yield of the extraction operation [30].…”

Section: Production Of Cellulasesmentioning

confidence: 99%

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Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Campos,

Asevedo,

Souza Filho

et al. 2024

Biomass

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The industrial processing of coconut to produce valuable foods, such as water and milk, generates large volumes of waste, especially the fruit shell. Despite this, material can be used in bioprocess applications, e.g., the production of enzymes, its recalcitrance hinders the cultivation of microorganisms, and low productivity is usually achieved. In this study, the production of cellulolytic enzymes through solid-state fermentation (SSF) and their extraction was investigated using the green coconut fiber pretreated by steam explosion, followed by alkali. The fungus Trichoderma reesei CCT-2768 was cultivated, using an experimental design, to study the effect of the water activity and the amount of biomass in the reactor. The combination of the pretreatment strategies yielded more porous biomass, with less hemicellulose (5.38%, compared to 10.15% of the raw biomass) and more cellulose (47.77% and 33.96% in the pretreated and raw biomasses, respectively). The water activity significantly affected the production of cellulases, with maximum activity yielded at the highest investigated value (0.995). Lastly, the extraction of the enzymes from the cultivation medium was studied, and a 9 g/L NaCl solution recovered the highest CMCase and FPase activities (5.19 and 1.19 U/g, respectively). This study provides an important contribution to the valorization of the coconut residue through (i) the application of the steam explosion technology to optimize the production of cellulases using the SSF technology and (ii) their extraction using different solvents.

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Section: Biomass Composition and Pretreatmentsupporting

confidence: 88%

Section: Steam Explosionmentioning

confidence: 99%

Section: Biomass Composition and Pretreatmentmentioning

confidence: 99%

Section: Production Of Cellulasesmentioning

confidence: 99%

See 2 more Smart Citations

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Campos,

Asevedo,

Souza Filho

et al. 2024

Biomass

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“…Some of the main strategies are summarized in Table 1. In addition, some authors have studied a combination of different pre-treatments to disrupt the lignocellulosic biomass (Costa et al 2019;Ribeiro et al 2022).…”

Section: Enzymes Used In the Process Of Bioethanol Production Enzymat...mentioning

confidence: 99%

Enzyme technology in bioethanol production from lignocellulosic biomass: Recent trends with a focus on immobilized enzymes

de Araújo,

Costa,

de Brito

et al. 2023

BioRes

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Enzyme immobilization is a useful tool to produce biocatalysts with improved performance, such as high activity, high stability at operational conditions, and easy recovery and reuse. In this context, the production of second-generation ethanol using immobilized enzymes was reviewed. Emphasis was placed on the pre-treatment and/or hydrolysis steps to increase efficiency and reduce the cost of the process. In addition, the process design of bioethanol using immobilized enzymes was critically reviewed. In the enzymatic pre-treatment, laccases, manganese peroxidases, lignin peroxidases, and lytic polysaccharide monooxygenases are the main enzymes involved. When considering processes with heterogeneous biocatalysts, laccases are the most explored enzyme. In the hydrolysis step, cellulases and hemicellulases or a mixture of them are the main enzymes explored in the literature frequently using magnetic nanoparticles as support for enzyme immobilization. Although enzyme immobilization is a mature technology, the use of these biocatalysts is frequently limited to only one step of the bioethanol production process, such as pre-treatment or hydrolysis performed using Benchmark Technology. However, some recent papers have explored innovative design processes using a mixture of immobilized enzymes or co-immobilized enzymes to join some process steps, thereby decreasing the cost of enzymes and equipment.

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Steam Explosion and Dilute Acid Pretreatments to Improve Enzymatic Hydrolysis and Ethanol Fermentation of Green Coconut Fibers

de Brito,

de Araújo,

Costa

et al. 2024

Waste Biomass Valor

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Ethanol production from green coconut fiber using a sequential steam explosion and alkaline pretreatment

Cited by 10 publications

References 42 publications

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Enzyme technology in bioethanol production from lignocellulosic biomass: Recent trends with a focus on immobilized enzymes

Steam Explosion and Dilute Acid Pretreatments to Improve Enzymatic Hydrolysis and Ethanol Fermentation of Green Coconut Fibers

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