Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage

Nayak, Guruprasad; Honguntikar, Sachin D.; Kalthur, Sneha Guruprasad; D’Souza, Antony Sylvan; Mutalik, Srinivas; Setty, M. Manjunath; Kalyankumar, Raksha; Krishnamurthy, Hanumanthappa; Adiga, Satish Kumar

doi:10.1016/j.jep.2016.02.003

Cited by 27 publications

(16 citation statements)

References 33 publications

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“…The Ckit expression in spermatogonial differentiation is required for the proliferation, differentiation and survival of these cells (Dolci, Pellegrini, Di Agostino, Geremia, & Rossi, ). Although it has been observed that the administration of CP (200 mg/kg, single dose) decreased the expression of this gene in prepubertal mice (Nayak et al., ), we observed that a lower dose administrated in adult life caused a slight increase in the expression of this gene in the testis, which was much more significant after the co‐treatment with A. aculeata or BC. Nayak et al.…”

Section: Discussioncontrasting

confidence: 77%

“…Nayak et al. () also observed an increase in the expression of this gene in prepubertal animals treated simultaneously with the CP and the Moringa oleifera extract and correlated this effect with the protection of fertility provided by this species. In addition, the Gstp1 (involved in detoxification of xenobiotics) induction by A. aculeata (CP+30) could attenuate CP toxicity.…”

Section: Discussionmentioning

confidence: 87%

“…This is due to the simplicity of this approach, the lower toxicity induced by these natural compounds (Mahajan, ) and also due to the limitations of the current methods for fertility preservation. Several studies have shown that natural products obtained from different plants can mitigate or prevent the negative impacts caused by chemotherapy on fertility and this approach can be considered safe (Ilbey et al., ; Kalthur et al., ; Nayak et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Acrocomia aculeataoil: Beneficial effects on cyclophosphamide-induced reproductive toxicity in male rats

et al. 2018

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This study aimed to evaluate the effects of the extracted oil of Acrocomia aculeata pulp in preventing or mitigating the reproductive toxicity induced by cyclophosphamide (CP) in male rats. Adult male rats were segregated into seven groups that received vehicle, 100 mg/kg/day of CP, or 10 mg/kg/day of β-carotene or 3 or 30 mg/kg/day of A. aculeata oil co-administered with CP. A. aculeata oil exhibited a high content of β-carotene. CP treatment induced reproductive toxicity in the animals, as it changed the reproductive organs weight, hormone levels, sperm counts and testicular histology. In contrast, co-administration of A. aculeata improved CP-induced alterations in these parameters. A. aculeata oil also increased the gene Ckit expression and normalised the antioxidant enzymes levels which were changed by CP. The A. aculeata oil is capable of protecting the male reproductive system from the adverse effects of CP, possibly by acting as an antioxidant and increasing the Ckit gene expression.

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Section: Discussioncontrasting

confidence: 77%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Acrocomia aculeataoil: Beneficial effects on cyclophosphamide-induced reproductive toxicity in male rats

et al. 2018

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“…Moringa oleifera leaf has also shown protective activities in spermatogonial cells and mitigates the cell damage of mice injected with cyclophosphamide (Nayak, Honguntikar, et al, 2016). The hexane extract of MOL has been reported to enhance seminiferous tubule, epididymis, testis, and seminal vesicle functions in male mice (Cajuday & Pocsidio, 2010).…”

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confidence: 99%

The beneficial effects of Moringa oleifera leaf on reproductive performance in mice

Zeng

Luo

Wang

et al. 2019

Food Science & Nutrition

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Moringa oleifera is a tropical plant with high nutritional and medicinal value. Recent studies have reported its remarkable effects in inflammatory, antioxidative, and anti‐diabetes modulations, but there was no significant report on its role in animal breeding. In this study, we investigated the effects of dietary Moringa oleifera leaf (MOL) on reproductive performances in mice. We studied the reproductive performance of mice for six consecutive gestations. Mice fed with 4% MOL diet showed improved litter size, litter birth weight, and litter survivals until weaning age compared to control mice fed with normal diet (p < 0.05). Mice fed with MOL diet did not change weight and organ coefficients. Serum malondialdehyde (MDA) concentrations in both male and female mice were significantly decreased by dietary MOL (p < 0.05), but glutathione peroxidase (GSH‐PX) and superoxide dismutase (SOD) were unchanged. For male, dietary MOL significantly reduced sperm abnormality rate (p < 0.05) and Bcl2‐associated X protein (Bax) expression in testis (p < 0.05), but did not affect serum testosterone and the expression levels of androgen receptor (AR), phosphoglycerate kinase 2 (Pgk2), protamine2 (Prm2), and B cell leukemia/lymphoma 2 (Bcl2) in testis. For female, dietary MOL did not change serum estradiol and the expressions of estrogen receptor beta (ERβ), Bcl2, Bax, and vascular endothelial growth factor receptor (VEGFR) in ovary. In summary, MOL increased litter size and antioxidant ability, reduced the rate of sperm abnormality and the expression of Bax. Therefore, MOL may serve as a functional feed addictive for improving animal reproductive performance.

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“…For instance, quercetin protected spermatogonial cells in the presence of oxidant agents [23]. Additionally, the ethanolic extract of Moringa oleifera reduced cyclophosphamide-induced damage on spermatogonial cells [24]. Results of previous studies also revealed that the essential oil of T. ammi caused morphological deformities of the sperm plasma membrane and detachment of head-to-tail coiling.…”

Section: Introductionmentioning

confidence: 91%

Effects of Monoterpenes of Trachyspermum ammi on the Viability of Spermatogonia Stem Cells In Vitro

Omidpanah

Aliakbari

Nabavi

et al. 2020

Plants

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Trachyspermum ammi (Apiaceae) plants have several medicinal and condimentary applications and are considered an aphrodisiac agent in Iranian Traditional Medicine. Thus, the present study aims to evaluate the effects of oil from Iranian T. ammi plants on the viability of spermatogonial stem cells in vitro. The essential oil of T. ammi fruits was extracted by hydrodistillation, and the amount of thymol was calculated by a gas-chromatography method. Spermatogonial stem cells were isolated from the testes of mice using enzyme digestion. Real-time polymerase chain reaction (RT-PCR) was applied to assess the gene expressions of promyelocytic leukemia zinc finger protein (Plzf), DNA-binding protein inhibitor (ID-4), tyrosine-protein kinase (c-Kit), B-cell lymphoma 2 (Bcl2) and Bcl2-associated X protein (BAX). The number and diameter of colonies were also measured in the treated cells. The amount of thymol in the oil was 130.7 ± 7.6 µg/mL. Flow cytometry analysis showed that 92.8% of all cells expressed stimulated by retinoic acid 8 (Stra8), a spermatogonial stem cell marker. Expression of Plzf and ID-4 genes significantly increased in the treatment groups, while c-Kit and BAX decreased, and Bcl2 increased in the presence of essential oil. The numbers and diameters of cells were also improved by the application of the plant oil. These data indicated that monoterpenes from the oil of T. ammi improved the quality and viability of spermatogonia cells in the cell culture.

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Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage

Cited by 27 publications

References 33 publications

Acrocomia aculeataoil: Beneficial effects on cyclophosphamide-induced reproductive toxicity in male rats

Acrocomia aculeataoil: Beneficial effects on cyclophosphamide-induced reproductive toxicity in male rats

The beneficial effects of Moringa oleifera leaf on reproductive performance in mice

Effects of Monoterpenes of Trachyspermum ammi on the Viability of Spermatogonia Stem Cells In Vitro

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