Ethephon and secondary shoot induction in Gentian (Gentiana spp.) hybrids in vitro

Keshavarzi, M.; Funnell, Keith A.; Heyes, J.A.; Woolley, D.J.

doi:10.1016/j.scienta.2014.09.020

Cited by 4 publications

(5 citation statements)

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“…Despite an earlier report of a potential correlation between the increases in gaseous ethylene in the headspace of culture vessels supplemented with ethephon and bud outgrowth of gentian (Keshavarzi et al, 2014), our study shows gaseous ethylene in the headspace was not effective on bud outgrowth (Fig. 4).…”

Section: Discussioncontrasting

confidence: 99%

“…This implies that the difference in sensitivity or response capacity of the tissue to ethephon drives differential cultivar responses (Firn, 1986). Together with results from previous experiments, ethephon is, therefore, unlikely to cause endogenous ethylene production in explants, but does trigger shoot formation in 'Little Pinkie' (Keshavarzi et al, 2014), perhaps through decomposition to ethylene and enhancement of the internal ethylene level possibly at a locally high concentration.…”

Section: Discussionmentioning

confidence: 55%

“…In the presence of ethephon, explants of 'Showtime Diva' produced a little more ethylene than explants of 'Little Pinkie'. Although this may imply that 'Showtime Diva' has the capacity for an increase in its own ethylene production under the influence of ethephon, it was only 'Little Pinkie' that responded to ethephon as a shoot formation stimulant (Keshavarzi et al, 2014). This implies that the difference in sensitivity or response capacity of the tissue to ethephon drives differential cultivar responses (Firn, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Hence, to investigate the current hypothesis in the first experiment we quantified the dynamics of ethylene concentration in the headspace of culture vessels without explants using laser ethylene detector technology. Our earlier research reported ethylene concentration at 48-h intervals using a method that removed accumulated ethylene in the headspace and replaced it with ethylene-free air at each measurement time (Keshavarzi et al, 2014). Within the current research, continuous measurement monitored ethylene concentration in real time, which relied on a continuous stream of ethylene-free gas passing over the headspace of culture vessels (Cristescu et al, 2008;Forni et al, 2012;Millenaar et al, 2009;van den Dungen et al, 2011).…”

mentioning

confidence: 99%

“…The number of shoots per propagule influences shoot predisposition to form a high-quality potted plant, and within an in vitro propagation system it directly determines the yield of nodal explants that can be used either for routine propagation to build up numbers or deflasked for production scheduling. In explants of Gentiana 'Little Pinkie' (naturally highly branched), shoot formation increased as a result of ethephon supplementation in vitro whereas shoot formation did not increase in explants of Gentiana 'Showtime Diva' (naturally less branched) (Keshavarzi et al, 2014). Regardless of the cultivar and whether grown in vivo or in vitro, information on growth and shoot branching in gentian is very limited.…”

mentioning

confidence: 99%

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Is Endogenous Ethylene Production Associated with Increased Shoot Number in Gentian Cultured In Vitro? Developing a Method for Ethylene Measurement at Nano Level within a Gas-exchangeable In Vitro System

Keshavarzi

Funnell

Woolley

et al. 2021

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We investigated the possibility of either exogenous ethylene or endogenous ethylene production having an association with the increase in shoot number when nodal explants of Gentiana spp. ‘Little Pinkie’ were cultured in an in vitro medium supplemented with ethephon (10 mg⋅L–1). For the first time within an in vitro system, we report the application of laser ethylene detector technology, and optimization of the methodology to quantify concentrations of ethylene (in the part-per-billion range) released from ethephon decomposition within the atmosphere of gas-exchangeable culture vessels including nodal explants. Compared with continuous (continuous measurements on the same replicate of vessels) and repeated (sampling same replicate of vessels every 48 hours) sampling methodologies, the nonrepeated (sampling fresh replicate of vessels every 48 hours) method of measurement of ethylene concentration was more representative of the actual condition within vessels. Although no prior published data exist showing the positive or negative effect of gaseous ethylene in the headspace of culture vessels on bud outgrowth in gentian, our study shows gaseous ethylene in the headspace of culture vessels was not effective in increasing shoot formation in gentian explants cultured in vitro, whereas ethephon supplementation in agar was effective. Plant material in culture vessels did not have a significant effect on ethylene production regardless of the presence or absence of ethephon. Therefore, although ethephon supplementation in the medium produced gaseous ethylene in the headspace, it was unlikely to cause endogenous ethylene production in explants, but it did trigger shoot formation in ‘Little Pinkie’, perhaps through decomposition to ethylene within the explant tissue, enhancing the internal ethylene level possibly at a locally high concentration.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 55%