(1) Background: the production of onion seeds is limited by the competition between seeds and the vegetative organs and by scape lodging. However, information on the effects of plant growth regulation on onion seed production is scarce. Aim of the present study was to evaluate the seed yield components and germination ability of onion seeds as affected by the timing and dose of an ethylene application, a plant growth regulator able to modulate shoot–flower competition; and chitosan, an elicitor of plant defense mechanisms able to increase its tolerance to various stresses. (2) Methods: Onion was treated with ethylene at the recommended dose (100% RD) of a commercial product, at 150% RD in two contrasting phenological phases or untreated (control), or ‘with’ or ‘without’ chitosan, and the seed yield components and germination trend were measured. (3) Results: 100% RD at an early phase of growth did not influence the seed yield and increased the thousand seed weight (TSW) by 3.2%. The application of 150% RD decreased the seed yield by 33.5%, and this occurred irrespective of the timing of application. Such decreases were due to a reduction in the number of seeds per flower. The application of chitosan did not affect the crop at 100% RD and increased the seed yield and slightly increased, but not significantly, the TSW under 150% RD. Germination of the fresh seed was 92%, and 17 months of aging reduced it by 14%, with no effects of the treatments on the germination pattern. (4) Conclusions: the ethylene application mostly affected TSW but not the yield, whereas high doses of ethylene reduced yields irrespective of the timing of application. Such a result may have been due to a delay in the flowering onset that occurred in a relatively dry month. Chitosan sustained its yield when the yield potential was reduced by 150% RD, and such a result was likely due to physical protection from the transpiration since the synthetic fungicides applied did not likely allow the pathogens to infections. These results have implications for establishing the timing and dose of application of plant growth regulators and elicitors in seed onions to sustain the seed quality.