Veillonella parvula ATCC 10790, an anaerobic gram-negative coccus, contains diacyl and alk-1-enyl acyl (plasmalogen) forms of phosphatidylethanolamine and phosphatidylserine. We studied the effect of growth temperature on the lipid composition of this strain. There was a small increase in the phosphatidylethanolamine content but no change in the content of plasmalogens at the lower growth temperatures tested. The total acyl chains and the plasmalogen acyl chains contained between 73 and 80% mono-unsaturated fatty acids at all growth temperatures. The plasmalogen alk-1-enyl chains were significantly more unsaturated in cells grown at 30 and 25°C than in cells grown at 37°C. Differential scanning calorimetry of the hydrated phospholipids showed lower phase transition temperatures for the lipids from the cells grown at the lower temperatures. In Megasphaera elsdenii lipids, which are similar in composition to the lipids of V. parvula, the proportion of phosphatidylethanolamine also increased slightly at lower growth temperatures, with no significant change in the content of plasmalogens. M. elsdenii contained cyclopropane fatty acyl and alk-1-enyl chains in addition to the mono-unsaturated and saturated chains previously reported. As cells entered the stationary phase of growth at 30 and 42.50C, there was a reciprocal increase in the proportion of cyclopropane acyl chains and decrease in the unsaturated moieties. The total proportion of cyclopropane and unsaturated acyl and alk-1-enyl chains was more than 65% at all growth temperatures studied, and there was no discernible increase in the sum of these moieties at the lower growth temperatures.1-Alkenyl-2-acyl glycerolipids (plasmalogens) are major constituents of certain mammalian tissues, including heart, brain white matter, peripheral nerves, and skeletal muscle. These lipids are also found in avian tissues and in many invertebrates (15,29,30). The presence of plasmalogens in procaryotes appears to be confined to strict anaerobes, in which they often represent a substantial fraction of the total lipid (9, 17, 31). Studies on the role of plasmalogens in the maintenance of membrane fluidity have only begun to probe the adaptive functions of these compounds. The pioneer work of Roots and Johnston (25,26) on the alkenyl ether lipid compositions of the neural tissues of goldfish acclimated to different temperatures suggested that the chain composition was regulated. Work in our laboratory has shown that both the acyl and alkenyl chains of the major plasmalogens of Clostridium butyricum, a strict anaerobe, undergo changes in composition as a function of growth temperature, as do the overall phospholipid composition and the plasmalogen contents of individual phospholipid classes (19, 20).These changes result in alterations in the phase behavior of the membrane phospholipids (10,11). To extend this work to other anaerobes with different phospholipid, acyl, and alkenyl compositions, we performed a study on the effects of growth temperature on the lipids of Veillonella parv...