Background: The professional life has been frequently made by social interactions in which different forms of professional collaboration appear. Everybody has different attitudes through his/her life that could influence on choosing profession and probably the approach towards inter professional work. In order to study the current attitude towards planning educational courses and assess efficiency of the ongoing educational courses, the present study has normalized the Persian version of the ‘Attitudes to Health Professionals Questionnaire’ (AHPQ).Method: After permission from questionnaire developers, in order to confirm face and content validity, the process of translation and back translation was performed. Then it was sent to ten scholars (Delphi Technique) and their comments were considered for providing the final copy. The questionnaire was back translation from Persian into English. The final version was compared with the original questionnaire in terms of concepts. Reliability confirm by test-retest and internal consistency (intra class correlation and Cronbach alpha coefficient).The construct validity was confirmed by exploratory factor analysis. Results: According to the first phase (67 participants), wide range has caused unnecessary variations in answers; so reliability has been confirmed in larger sample size (104 participants) after decreasing the extent of scale from 10 to 5 points. Exploratory factor analysis was used by main construct and rotation of maximum variance. Final reliability was confirmed by Cronbach alpha as 0. 899. Three underlying constructs of professional ethics, professional autonomy and patient-centered care had been revealed in the questionnaire.Conclusion: In addition to confirmation of AHPQ construct validity, reveals that such a tool is able to specify the attitude of health team members based on their perceived importance towards the basis of inter professional collaboration. Thus, recognizing and directing priorities of health care team in inter professional collaboration enables us to manage establishment, integrity, sustainability and coordination of group activities.