Ethyl acetate extract of Clausena execavatapromotes growth inhibition of Burkitt’s lymphoma cell line via apoptotic activities

Abstract

Clausena excavatais a famous folklore medicinal plant in Asian region that is being used for the treatment of different disorders. This study investigated the cytotoxic effects of leaf extracts via MTT assay, as well as the in vitroapoptotic activities of the ethyl acetate C. excavataleaf extract (EACE) on human Burkitt’s lymphoma, Raji, cell line using annexin-V-FITC/propidium iodide flow cytometric assays. Pro-apoptotic (BAX) and anti-apoptotic (BCL-2, c-MYC) gene expressions were determined via real-time quantitative PCR. Phytochemical screening was done by Gas chromatography-mass spectrometry (GC-MS). EACE has the lowest IC50(19.3 ± 0.35 μg/mL) among extracts. EACE-treated Raji cells after 48 h underwent apoptosis as evident by loss of cell viability and increase in the percentage of early and late apoptotic cells. The results also showed EACE mediated decreased in the BCL-2 and c-MYC gene expressions and increased in the BAX gene. C. excavatais a potential treatment for Burkitt lymphomathrough activation of apoptosis

Antiangiogenic Activity and Ros-Mediated Lung Cancer Cell Line Injury of Zerumbone

“…Negative treated cells didn't show increase in ROS whereas, H2O2 treated cells showed significant rise in fluorescence due to high ROS production (Figure 2). The present ROS induced cancer cell death result was similar to the previous manuscript in which ZER was reported to produce significant ROS from non-small cell lung cancer line beyond the protective effect of cellular anti-oxidant defense mechanism, resulting in damage to cell membrane and finally cell death [21,45]. ROS kill cancer cells by triggering both intrinsic and extrinsic apoptotic pathways [46].…”