Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis

Abstract

Context: Clausena excavata Burm. f is a plant used in folklore medicine for the treatment of various ailments in South East Asia. The plant parts contain chemical components that are cytotoxic to many cancer cells. Objective: The study investigated the cytotoxic effects of ethyl acetate, methanol and chloroform C. excavata leaf extracts on the nonsmall-lung cancer, NCI-H460, cell line. Methods: Based on the 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay, among extracts, ethyl acetate C. excavata leaf extract (EACE) was the most potent anti-NCI-H460 cells, with IC 50 value of 47.1 ± 6.1 μg/ml. The effects of EACE on NCI-H460 cells were also determined by clonogenic, 4', 6-diamidino-2-phenylindole (DAPI), and annexin-V-fluorescein isothiocyanate/ propidium iodide-PI flow cytometric assays. Reactive oxygen species (ROS) production and apoptotic gene expressions was determined via flow cytometry and real-time quantitative PCR, respectively. Results: EACE-treated NCI-H460 cells after 48 h underwent apoptosis as evident by loss of cell viability, cell shrinkage, and chromatin condensation. The results also showed EACE mediated increase in ROS production by the NCI-H460 cells. After 48 h treatment, EACE increased the pro-apoptotic BAX and decreased the anti-apoptotic Bcl-2, Survivin and c-Myc gene expressions. Conclusions: EACE is a potential anti-lung cancer by increasing cancer cell ROS production and apoptosis.

Antiangiogenic Activity and Ros-Mediated Lung Cancer Cell Line Injury of Zerumbone

“…In the present study, ZER exhibited TP inhibition with IC50 values of 50.3 ± 0.31 μg/ml or 230 ± 1.42 µM. The standard drug (7deazaxanthine) showed IC50 value of 41.0 ± 1.63 µM [23]. Based on the outcome of TP enzyme inhibition study, the potential of ZER to have antiangiogenic effect was observed.…”

“…The human lung cancer, NCI-H460, cell line was obtained from cell culture bio bank of Dr. Panjwani Center for Molecular Medicine and Drug Research, ICCBS, University of Karachi. The protocol of Albaayit et al [23] was followed in which cells were cultured and passaged using DMEM medium. MTT assay was used to find the cytotoxic effect of the ZER on the NCI-H460 cell lines.…”

The Anti-cancer Impact of Genetically Engineered Newcastle Disease Virus Expressing GFP Gene Against U87-MG Cell Line

“…Cancer is a category of diseases that can invade and spread to different sections of the body [1,2]. It is defined by abnormal cell proliferation.…”

“…Exogenous impacts include factors like chemicals, physical effects and biological. The term "Cancer" describes a class of disorders characterized by abnormal cell development and the capacity to invade or spread to different body regions [1,2].…”