Ethyl acetate extract of Streptomyces spp. isolated from Egyptian soil for management of Fusarium oxysporum: The causing agent of wilt disease of tomato

Abdel‐Aziz, Mohamed S.; Ghareeb, Mosad A.; Hamed, Ahmed A.; Rashad, Ehsan M.; El‐Sawy, Eslam R.; Saad, I.; Ghoneem, Khalid M.

doi:10.1016/j.bcab.2021.102185

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…Multiple previous studies have also highlighted the potential of actinobacteria to influence the growth of Fusarium spp. by generating hydrolytic enzymes [ 46 , 47 , 48 , 49 , 50 , 51 ]. Moreover, Streptomyces possess the capacity to inhabit plant root surfaces, survive in diverse soil types, and generate spores, enabling their prolonged existence even in varying extreme conditions [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Combatting Sugar Beet Root Rot: Streptomyces Strains’ Efficacy against Fusarium oxysporum

Abdelghany,

Yassin,

Abu-Ellail

et al. 2024

Plants

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Sugar beet root rot disease triggered by Fusarium oxysporum f. sp. radicis-betae is a destructive disease and dramatically affects the production and quality of the sugar beet industry. Employing beneficial microorganisms as a biocontrol strategy represents an eco-friendly and sustainable approach to combat various plant diseases. The distinct aspect of this study was to assess the antifungal and plant growth-promoting capabilities of recently isolated Streptomyces to treat sugar beet plants against infection with the phytopathogen F. oxysporum. Thirty-seven actinobacterial isolates were recovered from the rhizosphere of healthy sugar beet plants and screened for their potential to antagonize F. oxysporum in vitro. Two isolates SB3-15 and SB2-23 that displayed higher antagonistic effects were morphologically and molecularly identified as Streptomyces spp. Seed treatment with the fermentation broth of the selected Streptomyces strains SB3-15 and SB2-23 significantly reduced disease severity compared to the infected control in a greenhouse experiment. Streptomyces SB2-23 exhibited the highest protective activity with high efficacy ranging from 91.06 to 94.77% compared to chemical fungicide (86.44 to 92.36%). Furthermore, strain SB2-23 significantly increased plant weight, root weight, root length, and diameter. Likewise, it improves sucrose percentage and juice purity. As a consequence, the strain SB2-23’s intriguing biocontrol capability and sugar beet root growth stimulation present promising prospects for its utilization in both plant protection and enhancement strategies.

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Section: Discussionmentioning

confidence: 99%

Combatting Sugar Beet Root Rot: Streptomyces Strains’ Efficacy against Fusarium oxysporum

Abdelghany,

Yassin,

Abu-Ellail

et al. 2024

Plants

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“…The property of cell inhibition was similarly reported with the cytotoxic effect of the isolated Streptomyces crude extracts at a concentration varied from 0.39 to 50ug. The concentration of crude extracts leading to 50% cytotoxicity [25,26].…”

Section: Anti-proliferative Effect Of Crude Extract On Hepg2 Cell Linementioning

confidence: 99%

Isolation and Identification of Actinomycetes-A Potential Bioprospective Candidates

Bhagyasree S. R.,

Mary Helen P. A.

2023

UPJOZ

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Actinomycetes were isolated from the vegetable decomposing soil sample by serial dilution agar plating method. Five actinomycetes (A1 to A5) were obtained and the antimicrobial activity was carried out by Kirby Bauer agar diffusion method against selected eleven bacterial and eight fungal pathogens. Actinomycete strain A4 demonstrated the most activity. The most active actinomycete strain (A4) was identified phenotypically as Streptomyces spp. using macroscopic and microscopic examination. Following that, bioactive substances were purified using silica gel column chromatography and identified using Gas Chromatography-Mass Spectrometry. The major compounds identified were canadaline (35.79%), 2’4-diphromophenoxyphenol(31.07%). The ethyl acetate crude extract was assayed for anticancer activity by MTT method on HepG2cells with 10µl of extract showed 48.93% viability of HepG2 cells and significantly (p-value 0.000151 < 0.05 α-values) active among different concentration of crude A4 extract. The crude extract of A4 actinomyces exhibited the maximum inhibition of 47.55% at 10 10µl concentration and the activity were significant (p-value 0.01485) <0.05 α-values. The DNA was isolated from actinomycete strain (A4) and the 16SrRNA gene was amplified using universal eubacterial 16SrRNA gene primers and it was genotypically identified as Streptomyces coelicolor.

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“…Natural products (NPs) have a long history as a vital source of naturally occurring antimicrobial agents. These sources include marine, plant and microbial extracts, in addition to their bioactive secondary metabolites as well as essential oils [5][6][7][8][9][10][11][12][13] . Moreover, numerous classes of NPs have been evaluated for their antimicrobial effects against different types of pathogenic microbial strains including gram negative & gram positive bacteria, fungi and yeast.…”

Section: Introductionmentioning

confidence: 99%

Annona glabra fruit extracts: Chemical profiling and their potential antimicrobial activity against pathogenic microbial strains

Ghareeb

Khalaf²,

Abdel‐Aziz

et al. 2022

Egypt. J. Chem.

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Annona glabra L. is known for its edible fruit and it has numerous medicinal and therapeutic benefits. The aim of this study is to evaluate the antimicrobial activity of different solvent extracts of Annona glabra fruits followed by chemical profiling of the most bioactive extracts. The antimicrobial activity was estimated using disc agar plate diffusion test and chemical characterization was achieved using GC-MS analysis. Results revealed that all tested extracts exhibited noticeable antimicrobial activities with remarkable consideration to ethyl acetate, nbutanol and methanolic extracts. Ethyl acetate extract showed antimicrobial inhibition zone values ranged from 10 to 16mm. Whereas, n-butanol extract exhibited antimicrobial inhibition zone values ranged from 10 to 18mm. In addition, the methanolic extract displayed antimicrobial inhibition zone values ranged from 8 to 21mm, all against S. aureus, E. coli, C. albicans and A. niger. GC-MS analysis showed that 3,5-decadiyne, 2,2-dimethyl-(24.93%), hexadecanoic acid, methyl ester (12.92%), and hexadecanoic acid, methyl ester (23.69%), were detected as main chemical ingredients in the ethyl acetate, n-butanol, and methanol extracts, respectively. Annona glabra fruits could be a good source of natural antimicrobial agents.

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Ethyl acetate extract of Streptomyces spp. isolated from Egyptian soil for management of Fusarium oxysporum: The causing agent of wilt disease of tomato

Cited by 7 publications

References 40 publications

Combatting Sugar Beet Root Rot: Streptomyces Strains’ Efficacy against Fusarium oxysporum

Combatting Sugar Beet Root Rot: Streptomyces Strains’ Efficacy against Fusarium oxysporum

Isolation and Identification of Actinomycetes-A Potential Bioprospective Candidates

Annona glabra fruit extracts: Chemical profiling and their potential antimicrobial activity against pathogenic microbial strains

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