Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response

Abstract

The present study was designed to assess the antiarthritic potential of ECF in collagen-induced arthritis (CIA) and explore its underlying mechanism. Methods. In vitro, lymphocyte proliferation assay was measured by CCK-8 kit. In vivo, the therapeutic potential of ECF on CIA was investigated; surface marker, Treg cell, and intracellular cytokines (IL-17A and IFN-γ) were detected by flow cytometry. Th1 cell differentiation assay was performed, and mRNA expression in interferon-γ-related signaling was examined by q-PCR analysis. Results. In vitro, ECF markedly inhibited the proliferation of splenocytes in response to ConA and anti-CD3. In vivo, ECF treatment reduced the severity of CIA, inhibited IFN-γ and IL-6 secretion, and decreased the proportion of CD11b+Gr-1+ splenic neutrophil. Meanwhile, ECF treatment significantly inhibited the IFN-γ expression in CD4+T cell without obviously influencing the development of Th17 cells and T regulatory cells. In vitro, ECF suppressed the differentiation of naive CD4+ T cells into Th1. Furthermore, ECF intensely blocked the transcriptional expression in interferon-γ-related signaling, including IFN-γ, T-bet, STAT1, and STAT4. Conclusion. Our results indicated that ECF exerted antiarthritic potential in collagen-induced arthritis by suppressing Th1 immune response and interferon-γ-related signaling.

In-vitro and in-vivo antimalarial activity of caffeic acid and some of its derivatives

“…This alarming situation highlights the urgent need to discover new antimalarial molecules. Natural phenolic compounds, commonly found in various plants, and some of their simple esters are known for several biological activities, such as anti‐inflammatory, antioxidant, anti‐diabetic, anti‐carcinoma, antibacterial and immunomodulatory . Among phenolic metabolites, caffeic acid and derivatives (such as chlorogenic) are the most frequently found and could be present in high quantities in some plants used as antimalarial traditional medicines, but also in foods (coffee beans, thyme, mint, wine, several fruits and vegetables…) …”

ADMET and pharmaceutical activity analysis of caffeic acid diversities by in silico tools

“…Methyl caffeate can induce apoptosis by activation of caspase [27], as well as reduce low inflammation related to age which causes neurodegenerative diseases [28] such as Parkinson [14]. Ethyl caffeate can increasingly regulate caspase 3 and caspase 9 in tumor tissues [29] and also inhibits immune response and signaling, related to interferon γ, to cure arthritis, induced by collagen [30]. The current results show that the above mentioned caffeic acid diversities are proper for oral consumption with low toxic hazard.…”

Ethyl caffeate ameliorated amyloid‐beta42 protein‐associated toxicity inPC12cells andDrosophila melanogaster

“…29 EC also ameliorated collagen arthritis induced by collagen by subduing the T helper type 1 immune response. 30 Bearing in mind the implications of the innate immune neuroinflammation in AD pathology, anti-inflammatory therapy has become a target for AD. Thus, EC's neuroprotective effect on PC12 cells and the AD Drosophila might be linked with its antiinflammatory properties.…”