Ethyl cellulose nanocarriers and nanocrystals differentially deliver dexamethasone into intact, tape-stripped or sodium lauryl sulfate-exposed ex vivo human skin - assessment by intradermal microdialysis and extraction from the different skin layers

Döge, Nadine; Hönzke, Stefan; Schumacher, Fabian; Balzus, Benjamin; Colombo, Michela; Hadam, Sabrina; Rancan, Fiorenza; Blume-Peytavi, Ulrike; Schäfer‐Korting, Monika; Schindler, Anke; Rühl, E.; Skov, Per Stahl; Church, Martin K.; Hedtrich, Sarah; Kleuser, Burkhard; Bodmeier, Roland; Vogt, Annika

doi:10.1016/j.jconrel.2016.07.009

Cited by 63 publications

(37 citation statements)

References 46 publications

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“…Biomarker recovery was estimated by sampling through reference solutions contained in the inert skin matrix and different parameters were adjusted to study the effect on sampling of the 17 large biomarkers, which were chosen due to their relevance in dermal processes, such as wound healing and inflammatory skin conditions, including psoriasis and atopic dermatitis (19)(20)(21)(22)(23)(24). Other groups have used microdialysis in combination with thawed skin specimens to investigate absorption of topically applied drugs (25)(26)(27), but thawed skin has (to the best of our knowledge) never been used to evaluate sampling feasibility by skin microdialysis.…”

Section: Discussionmentioning

confidence: 99%

The Skin Reservoir Model: A Tool for Evaluating Microdialysis Sampling of Large Biomarkers from Human Skin

Baumann¹,

Falkencrone²,

Knudsen³

et al. 2020

Acta Derm Venerol

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Microdialysis is a minimally-invasive technique to study skin reactions by sampling of molecules from the tissue; however, not all molecules are easily recovered. Therefore, we propose a new model to investigate the sampling efficiency of larger biomarkers: the skin reservoir model. This model simulates the sampling situation in living human beings by using previously frozen excised human skin as a reservoir for biomarker solutions. Thus, it serves as a tool to carefully validate and optimize sampling of the target molecules before the use of microdialysis; for example, in clinical studies. Microdialysis is a well-established technique for sampling of small molecules from the human skin, but larger molecules are more difficult to recover. Consequently, sampling feasibility must be evaluated before microdialysis is used in vivo. This report presents a tool for estimating the recovery of large biomarkers from human skin by microdialysis, using previously frozen human skin specimens as reservoirs for biomarker reference solutions. Recovery of the following 17 biomarkers was assessed: CCL27/CTACK, CXCL1/GROα, CXCL7/ NAP-2, CXCL10/IP-10, EGF, GM-CSF, IFN-γ, IL-1α, IL-6, IL-8, IL-17, IL-22, IL-23, MIF, TNF-α, TSLP and VEGF. The relative skin recoveries of 13/17 biomarkers were successfully determined in the range 4.0-18.4%. Sampling in the skin reservoir model was not associated with probe leakage, as fluid recovery was stable, at between 80% and 110%. Furthermore, the skin reservoir model enabled studies and optimization of different parameters known to affect biomarker recovery, including flow rate and perfusate composition.

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Section: Discussionmentioning

confidence: 99%

The Skin Reservoir Model: A Tool for Evaluating Microdialysis Sampling of Large Biomarkers from Human Skin

Baumann¹,

Falkencrone²,

Knudsen³

et al. 2020

Acta Derm Venerol

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“…Alternatively, DXM compounds can be directly loaded in nanoparticles. A variety of nanoparticles prepared from biodegradable polymers such as PLGA, [28][29][30][31][32][33][34][35][36][37] PLLA, 38 PCL, 38 cellulose, 39 cyclodextrin, 40 chitosan, 41 polyglutamic acid, 42 or lipids 43 and inorganic materials, 10,44 as well as polymer micelles, 45 liposomes, [46][47][48][49] and carbon nanotubes 50 have been used to transport DXM. Entrapment of DXM in nanoparticles led usually to a prolonged and sustained release of DXM.…”

Section: Introductionmentioning

confidence: 99%

“…Entrapment of DXM in nanoparticles led usually to a prolonged and sustained release of DXM. 10,[30][31][32][33][34][35][36][37][38][39][40]44,50 Nevertheless, premature release of DXM before reaching the targeting tissues is still an issue. In order to minimize side effects due to leakage of drug and increase its bioavailability, an efficient encapsulation and a selective release drug delivery system are needed.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, most of the DXM nano-formulations were developed for topical therapies, e.g. the treatment of ophthalmic, 30,42,45,51 dermal, 10,39 respiratory, 32,52 bowel, 43,53 brain, 36,41 and joint diseases 18,24,28,29,[54][55][56] by local administration. In this case, the nanoencapsulation is mainly used for increasing local concentration of drugs and controlling their release kinetics.…”

Section: Introductionmentioning

confidence: 99%

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Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules

et al. 2020

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“…NC são isolados por ataque ácido preferencialmente hidrolisando as regiões amorfas de fibras de celulose, libertando os domínios nanométricos cristalino mais resistentes (MARIANO; EL KISSI; DUFRESNE, 2014).Por se tratar de um material leve, transparente, rígido, abundante e biodegradável têm despertado grande interesse comercial com vantagens competitivas em relação aos materiais fósseis e industrializados (KALIA; SABAA, 2013).Nanocristais de celulose tem sido aplicado em diversas áreas incluindo fármacos(DÖGE et al, 2016), embalagens(DA SILVA et al, 2015), medicina(DE FRANCE et al, 2016), e indústria têxtil (DP; BH, 2016).III -OBJETIVOSNeste trabalho foi proposto o desenvolvimento de blendas de goma de linhaça/ágar com menor solubilidade em água através da reticulação com glutaraldeído. Além disso, foi proposto a avaliação da inserção de nanocristais de celulose como agente de reforço com o intuito de melhorar o desempenho mecânico destas blendas.IV -PROCEDIMENTO EXPERIMENTALIV.1 MateriaisÁgar bacteriológico (Isofar), Semente de Linhaça dourada (comércio de Uberlândia-Brasil), Glicerol (Isofar), Glutaraldeído (solução 50% ;Sigma Aldrich), Ácido Clorídrico (Vetec), Pentóxido de fósforo (P2O5, 98,5 %; Sigma-Aldrich), Cloreto de Potássio (99-100 % ; Vetec), Polpa de madeira Kraft (Companhia Conpacel), Ácido Sulfúrico (95-98 % ; Vetec)…”

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Estudo da reticulação de blendas de goma de linhaça/ágar utilizando nanocristais de celulose como reforço

Prado¹

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AGRADECIMENTOSInicio meus agradecimentos a DEUS, por nesses dois anos ter colocado na minha vida pessoas tão especiais.Aos meus pais, Aurélio e Vânzia , meu infinito agradecimento pelos mais grandiosos ensinamentos que levo sempre comigo.Ao Luiz André que jamais mediu esforços para me ajudar.A Professora Dr. Harumi , pela orientação e contribuição.A meus amigos do LABREPOL, pelos momentos divididos juntos. Obrigada por compartilhar comigo dos sorrisos e angústias.A CAPES pelo apoio financeiro.A Doutoranda Ingrid por ter me ajudado ativamente com este trabalho, desde a extração dos nanocristais de celulose até a escolha de uma das matrizes de trabalho. Obrigada por nunca ter me negado ajuda quando precisei.A Doutoranda Ciências Exatas, Naturais e Educação -UFTM por disponibilizar o medidor de ângulo de contato e sua aluna Letícia pela ajuda nas medidas.Aos técnicos Roni Marcos, pelas análises termogravimétricas, Flaysner Magayver pelas análises de MEV, Mayta pela disponibilidade e gentileza. As Professoras Dras. Rosana Maria e Vivian Consuelo pelas contribuições no trabalho de qualificação de mestrado. Meu muito obrigada a todos! GLOSSÁRIO DE ABREVIATURAS E SIGLAS AGfilme de ágar AGGLI -filme de ágar com 30 % de glicerol AGGLI/GLA -filme de ágar com 30 % de glicerol e 15 % de glutaraldeído AGGLI/GLA/HClfilme de ágar com 30 % de glicerol e 15 % de glutaraldeído em meio ácido Ap -área de permeação ATR-FTIR -Refletância total atenuada acoplada a espectroscopia no infravermelho por transformada de Fourier XRD -Difração de Raios -X DSC -Calorimetria Exploratória Diferencial E -Módulo de Young εelongação na Ruptura eespessura EDS -Espectroscopia de energia dispersiva FAfração ácida FNfração neutra GL -filme de goma de linhaça GLA -glutaraldeído GLGLIfilme de goma de linhaça com 30 % de glicerol GLGLI/GLAfilme de goma de linhaça com 30 % de glicerol e 15 % de glutaraldeído GLGLI/GLA/HClfilme de goma de linhaça com 30 % de glicerol e 15 % de glutaraldeído em meio ácido. GLI -glicerol HCl -ácido clorídrico Jfluxo m -massa MEV -Microscopia Eletrônica de Varredura NC -nanocristal de celulose Pw -permeabilidade ao vapor de água R -constante dos gases TtemperaturaTGA -Análise Termogravimétrica absorção de água e melhora nas propriedades mecânicas. As formulações 50/502% NC e 50/504% NC apresentaram aumento da tensão na ruptura, elongamento e módulo de Young demonstrando assim resultados que justificam tanto a obtenção destas blendas quanto dos nanocompósitos. Além disso, com as diferentes morfologias obtidas nesse estudo é possível estender a faixa de aplicação para as formulações propostas.Palavras-chave: Goma de linhaça, ágar, blendas, reticulação, nanocristais de celulose. ABSTRACTNowadays, due to the incentive and the requirement for the use of sustainable products, the blends and nanocomposites from biopolymers appear as a proposal for application in several fields, such as: packaging, biomedicine and agriculture. In this sense, flaxseed gum/agar blends were developed using glutaraldehyde as a crosslinker and cellulose na...

show abstract

Ethyl cellulose nanocarriers and nanocrystals differentially deliver dexamethasone into intact, tape-stripped or sodium lauryl sulfate-exposed ex vivo human skin - assessment by intradermal microdialysis and extraction from the different skin layers

Cited by 63 publications

References 46 publications

The Skin Reservoir Model: A Tool for Evaluating Microdialysis Sampling of Large Biomarkers from Human Skin

The Skin Reservoir Model: A Tool for Evaluating Microdialysis Sampling of Large Biomarkers from Human Skin

Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules

Estudo da reticulação de blendas de goma de linhaça/ágar utilizando nanocristais de celulose como reforço

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