Ethyl Methanesulfonate and Caffeine Mutagenetic Treatment to Four Ornamental Silene Species

Li, Jiang; Dunn, Bruce L.

doi:10.24266/0738-2898-34.4.95

Cited by 4 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the breeding methods that can be applied to increase morphological diversity is artificial mutation. Mutation techniques have produced potential genotypes in various floricultural commodities [6,7]. One of the mutation sources that mutation breeders have widely used is ethyl methane sulphonate (EMS).…”

Section: Introductionmentioning

confidence: 99%

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Aisyah

Yudha²,

Sukma³

et al. 2023

J.Trop.Life.Science

View full text Add to dashboard Cite

Celosia cristata, an edible ornamental plant, is a potential floricultural commodity that needs further improvement to increase its agro-morphological characters and polyphenol content. Induced mutagenesis using ethyl methane sulphonate (EMS) is an effective tool to increase genetic diversity that has been applied in many plant species. This study aimed to assess the morphological diversity, polyphenol content, and antioxidant activities of C. cristata mutagenized by EMS in the M2 generation. A total of 230 M2 plants generated from the M1 generation were evaluated in this study and the polyphenols content and antioxidant activities analysis were conducted on fifteen selected M2 plants. Polyphenols content was analyzed using the Folin-Ciocalteu method and colorimetric method with slight modification, and the antioxidant activities investigated using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay with minor changes. There are six subpopulations with the highest diversity of quantitative characters in the M2 population in quantitative characters, i.e. C2-17-1.0%, C2-1-0.7%, C2-20-2.0%, C2-25-0.7%, C2-1-0.9%, and C2-1-0.7%. Changes in the shape and color of leaves, stems, and flowers of C. cristata were also observed in the M2 population. C2.1, C2.6, and C2.12. 2.1, C2.6, and C2.12 are potential plants derived from EMS mutagenesis with the highest polyphenol content and antioxidant capacity in the M2 population. In conclusion, induced mutation using EMS can enhance the agro-morphological diversity, polyphenols content along with the antioxidant activities of C. cristata, and demonstrate the successful mutation breeding program.

show abstract

Section: Introductionmentioning

confidence: 99%

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Aisyah

Yudha²,

Sukma³

et al. 2023

J.Trop.Life.Science

View full text Add to dashboard Cite

show abstract

“…The previous studies showed that using EMS could induce mutation in many ornamental plants such as rose [14], African violet [15], gerbera [16], Silene species [17], marigold [18] and chrysanthemum [19] also. Chrysanthemum is one of the most economically important cut flowers and ornamental plants across the global flower market.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ethyl Methanesulfonate on Mutation Induction in Chrysanthemum spp.

Puripunyavanich,

Chanchula,

Maikaeo

et al. 2023

Trends Sci

View full text Add to dashboard Cite

Mutation induction using EMS agent has been widely and successfully used for variation enhancement. In this study, the cultured plantlets from leaves of 7 chrysanthemum varieties were treated with 0, 1, 1.5, and 2 % ethyl methanesulfonate (EMS) solution for investigation of its efficacy for mutation induction. All treated explants were cultured on MS media supplemented with 2 mg/L 2,4D and 1 mg/L kinetin for callus induction and shoot regeneration. After the plants were cultured for 4 weeks, the percentage of shoot and root initiation and callus formation were observed. The varieties of M07-4, D27, 11-4 and 12-10 showed shoot/root regeneration and callus formation while the M07-14, D23 and 11-8 varieties showed shoot/root regeneration in some bottles. The surviving explants were transferred into the new MS medium supplemented with 1 mg/L BA and 0.1 mg/L NAA to obtain healthy plants ready for planting. The healthy plantlets were transferred to soil pot plants, and grown at Doi Khun Wang, Chiang Mai province, Thailand, for acclimatization. Morphological differentiation was evaluated after 16 weeks of planting in greenhouse. M07-14 varieties showed a maximum of 5 mutant characters. 11-8 varieties showed 4 mutant types while D23, D27, and 12-10 varieties produced 2 mutant types and 11-4 varieties showed only 1 mutant type. EMS proved to be effective in producing mutated chrysanthemum flowering plants that can lead to changes the color and form of flower from the original. HIGHLIGHTS Ethyl methanesulfonate (EMS) has been applied to induce mutation in chrysanthemum, which is ranked second in the global cut flower market, for enhancement in varieties and commercial value. EMS-induced mutation allowed various new characteristics of flower color and form dependent on EMS concentration and variety. All of chrysanthemum mutants which have the potential to be developed for commercial purpose were cultured in a tissue culture laboratory and prepared for commercial production in the next step. GRAPHICAL ABSTRACT

show abstract

Mutation Work on Other Ornamental Plants

Datta

2023

Role of Mutation Breeding in Floriculture Industry

View full text Add to dashboard Cite

Ethyl Methanesulfonate and Caffeine Mutagenetic Treatment to Four Ornamental Silene Species

Cited by 4 publications

References 13 publications

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Effects of Ethyl Methanesulfonate on Mutation Induction in Chrysanthemum spp.

Mutation Work on Other Ornamental Plants

Contact Info

Product

Resources

About