Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Joya-Dávila, J. G.; Gutiérrez-Miceli, Federico Antonio

doi:10.32604/phyton.2020.013679

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Espina et al [20] also investigated a phenotypic improvement of mutated soybean in the M2 population through induced mutation using EMS. In accordance with Joya-Dávila and Gutiérrez-Miceli [22], the highest genetic diversity in mutation breeding programs arises in the M2 generation. In this regard, the M2 generation is an important generation to gain as much diversity as is feasible.…”

Section: Quantitave Traitsmentioning

confidence: 62%

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Aisyah

Yudha²,

Sukma³

et al. 2023

J.Trop.Life.Science

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Celosia cristata, an edible ornamental plant, is a potential floricultural commodity that needs further improvement to increase its agro-morphological characters and polyphenol content. Induced mutagenesis using ethyl methane sulphonate (EMS) is an effective tool to increase genetic diversity that has been applied in many plant species. This study aimed to assess the morphological diversity, polyphenol content, and antioxidant activities of C. cristata mutagenized by EMS in the M2 generation. A total of 230 M2 plants generated from the M1 generation were evaluated in this study and the polyphenols content and antioxidant activities analysis were conducted on fifteen selected M2 plants. Polyphenols content was analyzed using the Folin-Ciocalteu method and colorimetric method with slight modification, and the antioxidant activities investigated using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay with minor changes. There are six subpopulations with the highest diversity of quantitative characters in the M2 population in quantitative characters, i.e. C2-17-1.0%, C2-1-0.7%, C2-20-2.0%, C2-25-0.7%, C2-1-0.9%, and C2-1-0.7%. Changes in the shape and color of leaves, stems, and flowers of C. cristata were also observed in the M2 population. C2.1, C2.6, and C2.12. 2.1, C2.6, and C2.12 are potential plants derived from EMS mutagenesis with the highest polyphenol content and antioxidant capacity in the M2 population. In conclusion, induced mutation using EMS can enhance the agro-morphological diversity, polyphenols content along with the antioxidant activities of C. cristata, and demonstrate the successful mutation breeding program.

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Section: Quantitave Traitsmentioning

confidence: 62%

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Aisyah

Yudha²,

Sukma³

et al. 2023

J.Trop.Life.Science

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Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2

Gubanova

2023

Agric. sci. pract.

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Aim. To identify mutations and evaluate the mutation frequency in Nigella damascena L. cultivars (cvs) Bereginya and Charivnytsya (M2 and M3 generation), following treatment of their seeds with ethyl methanesulfonate (EMS), nitrosomethylurea (NMU) and a new derivative of dimethyl sulfate, DG-2. Methods. Treated Nigella seeds of two cvs with the mutagens for 6 and 16 h and in concentrations of 0.01 and 0.5 % for EMS and NMU and 0.05 and 0.5 % for DG-2. Results. A wide range of mutations (59 types) was obtained, that was divided into six groups: five groups with changes in the morphological type and one group with changes in the physiological type. Among the detected mutations, there were both previously known mutations and those obtained in this culture for the first time. The high- est mutation frequency (30 %) affecting synthesis of chlorophyll and structure of stem, shoots and leaves and 20 % for physiological features, was registered for NMU at 16 h and 0.05 % in cv. Bereginya. However, this NMU concentra- tion appeared to be lethal for cv. Charivnytsya. Conclusions. The new mutagen DG-2 proved to be most effective for inducing mutations in the corolla petal color of nigella, namely 4.0 at a 0.5 % concentration of the mutagen and 16h exposure for cv. Bereginya and 4.0 % at the same concentration and exposure for cv. Charivnytsya. DG-2 caused a substantial number of mutations in all six mutation groups affecting morphological and physiological traits. The clas- sic mutagen EMS was also effective across the spectrum of mutation groups in our study; however, it caused mutations at a lower frequency. The maximum mutation frequency under influence of EMC at a concentration of 0.05 % and an exposure of 16 h in cv. Bereginya was 11.0 %, and in cv. Charivnytsya 8.0 %. For all three mutagens used, an increase in the concentration of the active substance and of exposure time led to an increase in the mutation frequency in N. damascena plants. We will select mutants with economically valuable traits, such as tall, lodging-resistant plants and early maturing ones, for further work on the development of new cultivars of N. damascena for industrial cultivation.

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Cambios bioquímicos y morfométricos en Coffea arabica posterior a un tratamiento con metanosulfonato de etilo

Joya Dávila,

Gutiérrez Miceli,

Luján Hidalgo

et al. 2023

BIOTECNIA

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El uso de metanosulfonato de etilo (EMS) es una tecnología utilizada para aumentar la variabilidad genética de cultivares. En el mejoramiento genético del cafeto, EMS podría generar nuevos rasgos fenotípicos y bioquímicos de importancia agrícola. El objetivo de la presente investigación fue determinar los cambios en la morfología y producción de metabolitos secundarios en plantas de cafeto generadas a partir de líneas celulares tratadas con EMS. Callos de Coffea arabica cv., Borbón fueron expuestos a nueve concentraciones de EMS, posteriormente, se determinó el porcentaje de supervivencia y la biomasa fresca. Se regeneraron plantas en medio MS suplementado con vitaminas, antioxidantes y 6-bencilaminopurina. A las 20 semanas se registraron cambios morfológicos observados con respecto a los callos no tratados con EMS. Empleando hojas secas se hizo la extracción de metabolitos secundarios, la cuantificación se realizó mediante cromatografía líquida de alta resolución. La supervivencia y crecimiento del callo disminuyó con el aumento de la concentración de EMS (dosis letal media: 148.8 mM), en plántulas generó cambios en la morfología de la hoja, enanismo y la formación de tres meristemas, que aumentarían el número de hojas. Finalmente, 140 mM de EMS incrementó el contenido de cafeína, ácido cafeico y ácido clorogénico.

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Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Cited by 3 publications

References 53 publications

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

Mutation types and frequency in Nigella damascena L. in the М2 and M3 generation, using ethyl methanesulfonate, nitrosomethylurea and a new derivative of dimethylsulfate, DG-2

Cambios bioquímicos y morfométricos en Coffea arabica posterior a un tratamiento con metanosulfonato de etilo

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