Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture obtained from contaminated groundwater

Waals, Marcelle J. van der; Plugge, Caroline M.; Meima-Franke, Marion; Waard, Pieter de; Bodelier, Paul L. E.; Smidt, Hauke; Gerritse, Jan

doi:10.1016/j.watres.2018.10.050

Cited by 26 publications

(12 citation statements)

References 47 publications

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“…Consequently, similarities in taxonomical composition of ETBE-degrading bacterial consortia from different studies and sites can be seen only on high taxonomic levels. Alpha-, Beta-and Gammaproteobacteria, Actinobacteria, Acidobacteria and Chloroflexi are reported in this and other studies [12,22], prompting to search candidate biodegrading bacteria within these groups.…”

Section: Discussionsupporting

confidence: 53%

“…The natural diversity of ethB may, however, remain hidden as the currently used ethB primers [7] are based on only three sequences. Indeed, van der Waals et al [22] did not detect ethB, when using primers based on A. tertiaricarbonis L108 sequence [50], in their ETBE-degrading cultures from polluted groundwater, despite the fact that ETBE-degradation was shown to be cytochrome P450-dependent. Besides the possibility that other than EthABCD system was involved, it is also conceivable that the primers used had not enough sequence complementarity with the ethB gene present.…”

Section: Discussionmentioning

confidence: 97%

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Distinct Bacterial Consortia Established in ETBE-Degrading Enrichments from a Polluted Aquifer

Kyselková

Salles

Dumestre³

et al. 2019

Applied Sciences

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Ethyl tert-butyl ether (ETBE) is a gasoline additive that became an important aquifer pollutant. The information about natural bacterial consortia with a capacity for complete ETBE degradation is limited. Here we assess the taxonomical composition of bacterial communities and diversity of the ethB gene (involved in ETBE biodegradation) in ETBE-enrichment cultures that were established from a gasoline-polluted aquifer, either from anoxic ETBE-polluted plume water (PW), or from an upstream non-polluted water (UW). We used a 16S rRNA microarray, and 16S rRNA and ethB gene sequencing. Despite the dissimilar initial chemical conditions and microbial composition, ETBE-degrading consortia were obtained from both PW and UW. The composition of ETBE-enrichment cultures was distinct from their initial water samples, reflecting the importance of the rare biosphere as a reservoir of potential ETBE degraders. No convergence was observed between the enrichment cultures originating from UW and PW, which were dominated by Mesorhizobium and Hydrogenophaga, respectively, indicating that distinct consortia with the same functional properties may be present at one site. Conserved ethB genes were evidenced in both PW and UW ETBE-enrichment cultures and in PW water. Our results suggest that the presence of ethB genes rather than the taxonomical composition of in situ bacterial communities indicate the potential for the ETBE degradation at a given site.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 97%

Distinct Bacterial Consortia Established in ETBE-Degrading Enrichments from a Polluted Aquifer

Kyselková

Salles

Dumestre³

et al. 2019

Applied Sciences

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“…Interestingly, a study investigating the bacterial colonisation of pristine sediments reported that members of the Comamonadaceae were early colonisers and therefore dominated the final microbial communities (Fillinger et al 2019 ). Furthermore, in a recent study, van der Waals et al ( 2019 ) identified Comamonadaceae as an abundant bacterial group in a mixed algal-bacterial culture obtained from contaminated groundwater that biodegraded ETBE in a batch reactor, suggesting that these microorganisms may play an important role in ETBE metabolism. To assess if the same microcosm ‘responders’ were identifiable at another ETBE-release site, this data was compared with Site T field data.…”

Section: Discussionmentioning

confidence: 99%

Distribution of ETBE-degrading microorganisms and functional capability in groundwater, and implications for characterising aquifer ETBE biodegradation potential

Nicholls

Rolfe

Mallinson

et al. 2021

Environ Sci Pollut Res

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Microbes in aquifers are present suspended in groundwater or attached to the aquifer sediment. Groundwater is often sampled at gasoline ether oxygenate (GEO)-impacted sites to assess the potential biodegradation of organic constituents. However, the distribution of GEO-degrading microorganisms between the groundwater and aquifer sediment must be understood to interpret this potential. In this study, the distribution of ethyl tert-butyl ether (ETBE)-degrading organisms and ETBE biodegradation potential was investigated in laboratory microcosm studies and mixed groundwater-aquifer sediment samples obtained from pumped monitoring wells at ETBE-impacted sites. ETBE biodegradation potential (as determined by quantification of the ethB gene) was detected predominantly in the attached microbial communities and was below detection limit in the groundwater communities. The copy number of ethB genes varied with borehole purge volume at the field sites. Members of the Comamonadaceae and Gammaproteobacteria families were identified as responders for ETBE biodegradation. However, the detection of the ethB gene is a more appropriate function-based indicator of ETBE biodegradation potential than taxonomic analysis of the microbial community. The study shows that a mixed groundwater-aquifer sediment (slurry) sample collected from monitoring wells after minimal purging can be used to assess the aquifer ETBE biodegradation potential at ETBE-release sites using this function-based concept.

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“…Studying the removal effect alone does not reveal a deeper mechanism. Based on the fact that most bacteria cannot be purified alone [7], the metabolic reactions of related bacteria are complex, and the variability of bacteria itself is strong, it is prone to draw wrong conclusions from the study of microbial community changes. By combining the removal effect with the change of microbial communities, the influence of environmental changes on the microbial communities of activated sludge can be more clearly revealed.…”

Section: Zhangxd [6]mentioning

confidence: 99%

Research on the Influence of Different Temperature Conditions on the Microbial Community in Anearobic-Anoxic-Oxic process on the Plateau

Huang

Zhang

et al. 2020

Preprint

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In order to further understand the influence of different temperature conditions in the low temperature range on the microbial community structure in the Anearobic-Anoxic-Oxic waste water treatment process on the plateau, four temperature conditions were designed in the research, including 25°C, 20°C, 15°C and 10°C. Each working condition lasted three days. Activated sludge from anaerobic tank, anoxic tank and oxic tank under each working condition was analyzed based on the 16S rRNA high-throughput sequencing technology. The result shows that the plateau temperature conditions have little influence on the level abundance of phylum. Under all conditions, Bacteroidetes, Proteobacteria and Actinobacteria are the main phyla. The abundance of nitrifying bacterium and phosphorus removal bacteria rose in the first three conditions and fell at 10 °C. The abundance of Denitrification bacteria and Nitrite oxidizing bacteria was significantly higher than that of Ammonia oxidation bacteria and Azotobacter bacteria and Phosphorus-accumulating Organisms(PAO) had an overall advantage over Glycogen-accumulating Organisms (GAO) throughout the research.

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Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture obtained from contaminated groundwater

Cited by 26 publications

References 47 publications

Distinct Bacterial Consortia Established in ETBE-Degrading Enrichments from a Polluted Aquifer

Distinct Bacterial Consortia Established in ETBE-Degrading Enrichments from a Polluted Aquifer

Distribution of ETBE-degrading microorganisms and functional capability in groundwater, and implications for characterising aquifer ETBE biodegradation potential

Research on the Influence of Different Temperature Conditions on the Microbial Community in Anearobic-Anoxic-Oxic process on the Plateau

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