Ethylene and auxin interaction in the control of adventitious rooting in<i>Arabidopsis thaliana</i>

Veloccia, A; Fattorini, Lorenzo; Rovere, Federica Della; Sofo, Adriano; D’Angeli, Simone; Betti, Camilla; Falasca, Giuseppina; Altamura, M. M.

doi:10.1093/jxb/erw415

Cited by 78 publications

(100 citation statements)

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“…2d and a, in comparison). This AR reduction was similar to that occurring in ech2ibr10 seedlings grown under the same IBA concentration and experimental conditions [9]. Mutant and WT TCLs treated with IAA (Fig.…”

Section: Resultssupporting

confidence: 80%

“…In Medicago truncatula , IAA treatments increase MtPIN1 and MtPIN2 expression, up-regulate most of the PINs in rice [43, 44], and positively affect PIN1 promoter activity in arabidopsis PR [45]. Coupled with the inhibition of AR formation reported for pin1 de-rooted seedlings [46], and the reduced AR response of lax3/aux1 IBA-alone-treated seedlings, and IBA + Kin-treated TCLs [8, 9], the present results about PIN1 and AUX1 expression (Fig. 5a-e, and k-o) and l a x3aux1 response (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In arabidopsis seedlings, by the use of the wei2wei7 mutant and the ASA1::GUS and ASB1::GUS lines, it has been shown that the anthranilate synthase is required for AR formation , with the transcriptional induction of the α-anthranilate synthase isoform (WEI2/ASA1) mainly involved [9]. However, it is unknown whether the same genes are involved in the AR-formation in TCLs .…”

Section: Introductionmentioning

confidence: 99%

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Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity

et al. 2017

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BackgroundIndole-3-acetic acid (IAA), and its precursor indole-3-butyric acid (IBA), control adventitious root (AR) formation in planta. Adventitious roots are also crucial for propagation via cuttings. However, IBA role(s) is/are still far to be elucidated. In Arabidopsis thaliana stem cuttings, 10 μM IBA is more AR-inductive than 10 μM IAA, and, in thin cell layers (TCLs), IBA induces ARs when combined with 0.1 μM kinetin (Kin). It is unknown whether arabidopsis TCLs produce ARs under IBA alone (10 μM) or IAA alone (10 μM), and whether they contain endogenous IAA/IBA at culture onset, possibly interfering with the exogenous IBA/IAA input. Moreover, it is unknown whether an IBA-to-IAA conversion is active in TCLs, and positively affects AR formation, possibly through the activity of the nitric oxide (NO) deriving from the conversion process.ResultsRevealed undetectable levels of both auxins at culture onset, showing that arabidopsis TCLs were optimal for investigating AR-formation under the total control of exogenous auxins. The AR-response of TCLs from various ecotypes, transgenic lines and knockout mutants was analyzed under different treatments. It was shown that ARs are better induced by IBA than IAA and IBA + Kin. IBA induced IAA-efflux (PIN1) and IAA-influx (AUX1/LAX3) genes, IAA-influx carriers activities, and expression of ANTHRANILATE SYNTHASE -alpha1 (ASA1), a gene involved in IAA-biosynthesis. ASA1 and ANTHRANILATE SYNTHASE -beta1 (ASB1), the other subunit of the same enzyme, positively affected AR-formation in the presence of exogenous IBA, because the AR-response in the TCLs of their mutant wei2wei7 was highly reduced. The AR-response of IBA-treated TCLs from ech2ibr10 mutant, blocked into IBA-to-IAA-conversion, was also strongly reduced. Nitric oxide, an IAA downstream signal and a by-product of IBA-to-IAA conversion, was early detected in IAA- and IBA-treated TCLs, but at higher levels in the latter explants.ConclusionsAltogether, results showed that IBA induced AR-formation by conversion into IAA involving NO activity, and by a positive action on IAA-transport and ASA1/ASB1-mediated IAA-biosynthesis. Results are important for applications aimed to overcome rooting recalcitrance in species of economic value, but mainly for helping to understand IBA involvement in the natural process of adventitious rooting.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1071-x) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity

et al. 2017

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“…Unlike hypoxia, ethylene inhibits AR initiation (Veloccia et al . ). High levels of ethylene inhibited AR initiation to the same degree in normoxic and hypoxic conditions, indicating that ethylene can override the hypoxia signal (Figure S4).…”

Section: Discussionmentioning

confidence: 97%

“…In normoxia, AR growth is inhibited by ethylene in a dose‐dependent manner (Veloccia et al . ), suggesting that at lower ethylene levels AR are formed that can elongate faster when exposed to hypoxia. Future work may clarify if the sensitivity to ethylene changes under hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation inArabidopsis

Eysholdt-Derzsó

Sauter

2018

Plant Biol J

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Soil water-logging and flooding are common environmental stress conditions that can impair plant fitness. Roots are the first organs to be confronted with reduced oxygen tension as a result of flooding. While anatomical and morphological adaptations of roots are extensively studied, the root system architecture is only now becoming a focus of flooding research. Adventitious root (AR) formation shifts the root system higher up the plant, thereby facilitating supply with oxygen, and thus improving root and plant survival. We used Arabidopsis knockout mutants and overexpressors of ERFVII transcription factors to study their role in AR formation under hypoxic conditions and in response to ethylene. Results show that ethylene inhibits AR formation. Hypoxia mainly promotes AR elongation rather than formation mediated by ERFVII transcription factors, as indicated by reduced AR elongation in erfVII seedlings. Overexpression of HRE2 induces AR elongation to the same degree as hypoxia, while ethylene overrides HRE2-induced AR elongation. The ERFVII transcription factors promote establishment of an AR system that is under negative control by ethylene. Inhibition of growth of the main root system and promotion of AR elongation under hypoxia strengthens the root system in upper soil layers where oxygen shortage may last for shorter time periods.

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Role of Plant Growth Regulators (PGRs) in Mitigation of Heavy Metal Phytotoxicity in Plants

Singh

Sharma

Kohli

et al. 2020

Cellular and Molecular Phytotoxicity of Heavy Metals

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Ethylene and auxin interaction in the control of adventitious rooting inArabidopsis thaliana

Abstract: HighlightEthylene affects adventitious rooting by reducing indole-3-acetic acid (IAA) biosynthesis, but enhancing conversion into IAA of its precursor indole-3-butyric acid (IBA). This conversion, together with active IAA-cellular-influx, is essential for adventitious root formation.

Cited by 78 publications

References 41 publications

Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity

Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity

Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation inArabidopsis

Role of Plant Growth Regulators (PGRs) in Mitigation of Heavy Metal Phytotoxicity in Plants

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