Ethylene and cytokinin interaction in the morphogenesis of Pelargonium × hortorum L.H. Bailey in vitro

Wojtania, Agnieszka; Wegrzynowicz-Lesiak, E.

doi:10.1007/s11738-012-1005-z

Cited by 7 publications

(9 citation statements)

References 22 publications

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“…As shown in our study, the addition of AVG (inhibitor of ACC synthase) to mTmedium also did not have a significant influence on Pelargonium shoot formation, although a very low ethylene production was observed in that treatment. In contrast to AVG, another ethylene inhibitor, α-aminooxyacetic acid (AOA), caused a reduction in Pelargonium shoot formation (Wojtania and Węgrzynowicz-Lesiak, 2012). This was probably due to other activities of ethylene inhibitors in plant metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…Among the various Pelargonium genotypes cultured in vitro, P. × hortorum 'Bergpalais' is distinguished by a low sensitivity to ethylene and a tendency to experience the inhibition of shoot formation at the initial and multiplication stages (Wojtania and Węgrzynowicz-Lesiak, 2012). Our previous study had shown that mT-stimulation shoot formation in Pelargonium in vitro correlated with increased ethylene production at the beginning of the subculture period.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous study showed that ethylene has an important influence on Pelargonium morphogenesis in vitro (Wojtania and Węgrzynowicz-Lesiak, 2012). It was found that the cytokinin-stimulation of the shoot formation was associated with an ethylene increase at the beginning of the culture period.…”

Section: Inroductionmentioning

confidence: 98%

“…The experiments were performed with Pelargonium × hortorum 'Bergpalais', characterized by low sensitivity to ethylene in vitro (Wojtania and Węgrzynowicz-Lesiak, 2012). Plant material for the experiment was derived from stock cultures initiated from shoot tips and axillary buds, and multiplied for one year on a medium containing meta-topolin (2.07 μM) and 30 g L -1 sucrose.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…The shoots were subcultured at three week intervals into a fresh medium in an Erlenmeyer flask and kept at a temperature of 23°C, under a 16 h photoperiod provided by cool-white fluorescent lamps at 40 μmol m ) without growth regulators (control) or supplemented with meta-topolin (mT, 2.07 μM) alone or together with a precursor of ethylene biosynthesis -1 aminocyclopropane-1-carboxylic acid (ACC, 9.9 μM), an inhibitor of ethylene synthesis -aminoethoxyvinylglycine (AVG, 5.1 μM), or an inhibitor of ethylene action -AgNO 3 (5.9 μM). The concentration of growth regulators was determined on the basis of previous experiments (Wojtania, 2010;Wojtania and Węgrzynowicz-Lesiak, 2012).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Wojtania¹,

Wegrzynowicz-Lesiak²,

Dziurka³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L -1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO 3 ), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15-40 g L -1) in the presence of mT and AgNO 3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L -1 ) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.K Ke ey y w wo or rd ds s: : Ethylene inhibitors, endogenous ABA, geranium, meta-topolin, sucrose concentration, shoot multiplication.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Inroductionmentioning

confidence: 98%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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