Ethylene Biosynthesis

Kende, Hans

doi:10.1146/annurev.pp.44.060193.001435

Cited by 1,034 publications

(488 citation statements)

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“…In the predicted LlACS, there were seven evolutionarily conserved regions found in all ACC synthases (Fig. S1d) [29][30][31][32], and conserved amino acid residues that are important for enzymatic activity [18,19], e.g., the Lys residue (V region) contained in the active center and involved in cofactor (PLP) and substrate (SAM) binding [17], as well as the Glu residue (I region) responsible for substrate specificity [33]. The Ser residue, located at Position 460 of the LlACS protein, is a part of the characteristic tripeptide R/K-L/V-S and may, similarly to ACC synthases from other plant species, be the place of phosphorylation [34].…”

Section: Discussionmentioning

confidence: 99%

Ethylene-dependent effects on generative organ abscission of Lupinus luteus

et al. 2017

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The abscission of certain organs from the plant is part of the fulfilment of its developmental programs. The separation process occurs in a specialized abscission zone usually formed at the base of detached organ. The changing level of phytohormones, particularly ethylene, is the element responsible for coordinating anatomical and physiological transformation that accompanies organ abscission. The application of ethylene (ET) on Lupinus luteus stimulates flower abortion. However, the treatment with 1-aminocyclopropane-1-carboxylic acid (ACC) -direct ET precursor -does not cause such a strong physiological response. In turn, when applied on the pedicels both ET biosynthesis (2-aminoethoxyvinylglycine; AVG) and action (norbornadiene; NBD) inhibitors reversed the stimulatory effect of ET on generative organ separation. In order to determine ET role in the flower abscission process in L. luteus, we identified the sequences coding for synthase (LlACS) and oxidase (LlACO) of ACC and measured their expression levels. Abscission zone activation is accompanied by a considerable increase both in LlACS and LlACO cDNAs and also ACC content, which is specifically localized in the dividing cells at the base of the flower being detached. Obtained results suggest that ET is a strong stimulator of flower abortion in L. luteus.

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Section: Discussionmentioning

confidence: 99%

Ethylene-dependent effects on generative organ abscission of Lupinus luteus

et al. 2017

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“…documented in plants. [42][43][44] Ethylene is derived from the amino acid Met. Methionine is converted to SAM by the enzyme SAM synthetase.…”

Section: Introductionmentioning

confidence: 99%

“…1). The final conversion of ACC to ethylene is oxygen dependent 43 and yields CO 2 and cyanide as by-products. ACC oxidase may play an important role in regulating ethylene biosynthesis, especially during conditions of high ethylene production.…”

Section: Introductionmentioning

confidence: 99%

Cross-talk between sulfur assimilation and ethylene signaling in plants

Iqbal¹,

Masood

Khan³

et al. 2013

Plant Signaling & Behavior

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“…The activity of ACC synthase can be induced 10-fold by wounding and treatment with LiC1 [8]. This increase in activity is due to de novo synthesis of this enzyme [20].…”

Section: Resultsmentioning

confidence: 99%

“…cDNAs encoding ACC synthases were later isolated by screening a cDNA library using either polyclonal antibodies [3,4] or degenerated oligonucleotides [5,6]. To date, ACC synthase cDNAs have been isolated from many crops of both agricultural and horticultural importance [7,8].…”

Section: Introductionmentioning

confidence: 99%

Effects of N‐terminal deletions on 1‐aminocyclopropane‐1‐carboxylate synthase activity

Huxtable

Yang

et al. 1996

FEBS Letters

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A series of nested N-terminal deletions were made on the full-length (wt) and C-terminal deleted (Cdel) 1-aminocyclopropane-l-carboxylate synthase cDNAs. These wt and mutant ACC synthases were over-expressed in a heterologous E. coil expression system. It was found that removal of an amino acid region (residues 2-12) from the non-conserved N-termini of wt and Cdel ACC synthases led to a slight increase in both in vivo ACC production and in vitro ACC synthase activity. Further deletion of 11 amino acids through GIn-23 from the N-termini of both wt and Cdel ACC synthases resulted in a substantial reduction in both in vivo ACC production and in vitro enzyme activity. Deletion of an amino acid region, residues 3 through 27, from the N-terminus of ACC synthase abolished enzyme activity completely. Kinetic analysis of a highly purified double-deletion mutant (NCdel-1) of ACC synthase demonstrated that the Km of this mutant is 42 ~M, which is much smaller than that of the corresponding Cdel (280 /tM) and closer to that of wt (22 tiM) reported previously, suggesting a clear effect of the non-conserved N-terminal region on its ACC synthase function.

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Ethylene Biosynthesis

Cited by 1,034 publications

References 0 publications

Ethylene-dependent effects on generative organ abscission of Lupinus luteus

Ethylene-dependent effects on generative organ abscission of Lupinus luteus

Cross-talk between sulfur assimilation and ethylene signaling in plants

Effects of N‐terminal deletions on 1‐aminocyclopropane‐1‐carboxylate synthase activity

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