Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Najafi, Abouzar; Kia, Hossein Daghigh; Dodaran, Hossein Vaseghi; Mehdipour, Mahdieh; Álvarez‐Rodríguez, Manuel

doi:10.1016/j.anireprosci.2016.12.004

Cited by 26 publications

(13 citation statements)

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“…Mitochondrial integrity was measured by rhodhamine 123 as described previously 59 . Briefly, spermatozoa was washed with PBS and adjusted to 5 × 10 6 /mL concentration.…”

Section: Methodsmentioning

confidence: 99%

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Peroxiredoxin activity is a major landmark of male fertility

Ryu

Kim

Kwon

et al. 2017

Sci Rep

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Peroxiredoxins (PRDXs) are important antioxidant enzymes reported to have a role in sperm function and male fertility. However, how PRDXs affects male fertility remain fundamental unanswered questions. We therefore sought to investigate the role of these enzymes in sperm function and fertilisation. In this in vitro trial, mouse spermatozoa were incubated with different concentrations of conoidin A (1, 10, or 100 µM), a specific inhibitor of PRDXs. Our results demonstrated that inhibition of PRDXs by conoidin A significantly decreased the oxidized form of peroxiredoxins (PRDXs-SO3) in spermatozoa. Decreased PRDX activity was associated with a significant reduction in sperm motility parameters, viability, and intracellular ATP, whereas ROS levels, DNA fragmentation, and loss of mitochondrial membrane potential were increased. Simultaneously capacitation and the acrosome reaction were also significantly inhibited perhaps as a consequence of decreased tyrosine phosphorylation and protein kinase-A activity. In addition, fertilisation and early embryonic development were adversely affected following PRDXs inhibition in spermatozoa. Taken together, our data demonstrate that decreased PRDX activity directly affects male fertility due to negative effects on important functions and biochemical properties of spermatozoa, ultimately leading to poor fertilisation and embryonic development.

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“…Mitochondrial integrity was measured by rhodhamine 123 as described previously 59 . Briefly, spermatozoa was washed with PBS and adjusted to 5 × 10 6 /mL concentration.…”

Section: Methodsmentioning

confidence: 99%

“…The fluorescence intensities of Rh123 and PI of 10,000 events were recorded for each treatment. Spermatozoa with normal mitochondrial integrity, which is expressed by positive signal for Rh123 and negative signal for PI was calculated 59 , 60 .…”

Section: Methodsmentioning

confidence: 99%

Peroxiredoxin activity is a major landmark of male fertility

Ryu

Kim

Kwon

et al. 2017

Sci Rep

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“…Furthermore, sperm velocity distribution was analyzed by identifying four sperm cell movement subcategories based on ram-specific VAP cutoff, as reported in the HTM-IVOS CASA system, version 12.3 software: percentage of rapid cells (fraction of cells moving with VAP >75 μm/s; rapid, %); percentage of medium speed cells (fraction of cells moving with VAP values ranging from 21.9 to 75 μm/s; medium, %); percentage of slow cells (fraction of cells with VAP <21.9-75 μm/s; slow, %) and percentage of static cells (fraction of cells not moving at all; static, %). Sperm motility parameters were defined as previously reported in the IVOS software manual, and in Mortimer and Maxwell (1999), Palacin et al (2013), Najafi et al (2017). Statistical comparisons were made by using the unpaired Student's t-test.…”

Section: Sperm Motility Assessment By Computer-assisted Sperm Analysimentioning

confidence: 99%

Effect of cariporide on ram sperm pH regulation and motility: possible role of NHE1

et al. 2018

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Sperm motility, a feature essential for fertilization, is influenced by intracellular pH (pH) homeostasis. Several mechanisms are involved in pH regulation, among which sodium-hydrogen exchangers (NHEs), a family of integral transmembrane proteins that catalyze the exchange of Na for H across lipid bilayers. A preliminary characterization of NHE activity and kinetic parameters, followed by analysis of the expression and localization of the protein in ram spermatozoa was performed. NHE activity showed an apparent for external Na of 17.61 mM. Immunoblotting revealed a molecular mass of 85 kDa. Immunolocalization pattern showed some species-specific aspects, such as positive labeling at the equatorial region of the sperm head. Cariporide, a selective NHE1 inhibitor, significantly reduced pH recovery (85%). Similarly, exposure to cariporide significantly inhibited different motility parameters, including those related to sperm capacitation. fertilization (IVF) was not affected by cariporide, possibly due to the non-dramatic, although significant, drop in motility and velocity parameters or due to prolonged exposure during IVF, which may have caused progressive loss of its inhibitory effect. In conclusion, this is the first study documenting, in a large animal model (sheep) of well-known translational relevance, a direct functional role of NHE on sperm pH and motility. The postulated specificity of cariporide toward isoform 1 of the Na/H exchanger seems to suggest that NHE1 may contribute to the observed effects on sperm cell functionality.

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“…Lecithin protects the sperm plasma membrane by restoring the phospholipids lost during the heat shock (Toker et al, 2016). Many researchers have obtained acceptable semen parameters with using lecithin‐based extenders at post‐thaw and after incubation (Forouzanfar et al, 2010; Najafi, Daghigh‐Kia, Dodaran, Mehdipour, & Alvarez‐Rodriguez, 2017; Toker et al, 2016; Ustuner et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post‐thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin‐based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 × 106 spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA‐FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post‐thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin‐based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.

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Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Cited by 26 publications

References 50 publications

Peroxiredoxin activity is a major landmark of male fertility

Peroxiredoxin activity is a major landmark of male fertility

Effect of cariporide on ram sperm pH regulation and motility: possible role of NHE1

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

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