Ethylene glycol dimethacrylate and diethylene glycol dimethacrylate exhibits cytotoxic and genotoxic effect on human gingival fibroblasts via induction of reactive oxygen species

Bielecka-Kowalska, Anna; Czarny, Piotr; Wigner, Paulina; Synowiec, Ewelina; Kowalski, Bartosz; Szwed, Marzena; Krupa, Renata; Toma, Monika; Drzewiecka, Malgorzata; Majsterek, Ireneusz; Szemraj, Janusz; Śliwiński, Tomasz; Kowalski, Michał

doi:10.1016/j.tiv.2017.10.028

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…They recommended using a concentration of 0.1% instead of 1.5% TEOA to avoid toxic effects despite successful polymerization. The cytotoxicity of non-polymerized EGDMA was also determined by Bielecka et al [75] for human gingival fibroblasts using an incubation time of 24 h. In contrast, high cell viability of isolated murine and human chondrocytes could be detected when EGDMA was pre-polymerized [76,77]. A study by Chien et al [78] demonstrated that SBMA and MPC monomers exhibited low cytotoxic effects for a murine fibroblast cell line compared to other methacrylated monomers.…”

Section: Biocompatibilitymentioning

confidence: 93%

In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes

Kock

Striegl

Kraus³

et al. 2023

Bioengineering

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Osteoarthritis (OA) is a degenerative joint disease causing loss of articular cartilage and structural damage in all joint tissues. Given the limited regenerative capacity of articular cartilage, methods to support the native structural properties of articular cartilage are highly anticipated. The aim of this study was to infiltrate zwitterionic monomer solutions into human OA-cartilage explants to replace lost proteoglycans. The study included polymerization and deposition of methacryloyloxyethyl-phosphorylcholine- and a novel sulfobetaine-methacrylate-based monomer solution within ex vivo human OA-cartilage explants and the encapsulation of isolated chondrocytes within hydrogels and the corresponding effects on chondrocyte viability. The results demonstrated that zwitterionic cartilage–hydrogel networks are formed by infiltration. In general, cytotoxic effects of the monomer solutions were observed, as was a time-dependent infiltration behavior into the tissue accompanied by increasing cell death and penetration depth. The successful deposition of zwitterionic hydrogels within OA cartilage identifies the infiltration method as a potential future therapeutic option for the repair/replacement of OA-cartilage extracellular suprastructure. Due to the toxic effects of the monomer solutions, the focus should be on sealing the OA-cartilage surface, instead of complete infiltration. An alternative treatment option for focal cartilage defects could be the usage of monomer solutions, especially the novel generated sulfobetaine-methacrylate-based monomer solution, as bionic for cell-based 3D bioprintable hydrogels.

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Section: Biocompatibilitymentioning

confidence: 93%

In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes

Kock

Striegl

Kraus³

et al. 2023

Bioengineering

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“…2-propenoic acid, 1,2-ethanediyl ester [ 35 ] may have been used in 3D polyjet printing as a component in photopolymer resins due to its ability to form cross-linked structures upon exposure to UV light. This cross-linking enhances the mechanical strength and durability of the printed objects.…”

Section: Methodsmentioning

confidence: 99%

Performance of Fabrics with 3D-Printed Photosensitive Acrylic Resin on the Surface

Becker,

Ciesielska-Wrόbel

2024

Polymers

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Additive manufacturing (AM), also known as three-dimensional printing (3DP), has been widely applied to various fields and industries, including automotive, healthcare, and rapid prototyping. This study evaluates the effects of 3DP on textile properties. The usability of a textile and its durability are determined by its strength, washability, colorfastness to light, and abrasion resistance, among other traits, which may be impacted by the application of 3DP on the fabric’s surface. This study examines the application of photosensitive acrylic resin on two fabric substrates: 100% cotton and 100% polyester white woven fabrics made of yarns with staple fibers. A simple alphanumeric text was translated into braille and the braille dots were 3D printed onto both fabrics. The color of the printed photosensitive acrylic resin was black, and it was an equal mixture of VeroCyanV, VeroYellowV, and VeroMagentaV. The 3D-printed design was the same on both fabrics and was composed of braille dots with a domed top. Both of the 3DP fabrics passed the colorfastness to washing test with no transfer or color change, but 3D prints on both fabrics showed significant color change during the colorfastness to light test. The tensile strength tests indicated an overall reduction in strength and elongation when the fabrics had 3DP on their surface. An abrasion resistance test revealed that the resin had a stronger adhesion to the cotton than to the polyester, but both resins were removed from the fabric with the abrader. These findings suggest that while 3DP on textiles offers unique possibilities for customization and design, mechanical properties and color stability trade-offs need to be considered. Further evaluation of textiles and 3D prints of textiles and their performance in areas such as colorfastness and durability are warranted to harness the full potential of this technology in the fashion and textile industry.

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“…After incubation of cell lines with HAL, PLEC and MRC we assessed caspase-3/7 activity by fluorescence microplate reader using the commercial CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen™) that is based on the emission of fluorescence upon cleavage of the fluorescently labeled DEVD peptide. We followed the procedure as we described earlier [ 67 , 68 ]. Briefly, MCF-7 and FaDu cells were seeded in 96-well microplate with transparent bottoms at 1×10 5 cells/well in 50 µL culture medium and incubated at 37 °C, 5% CO 2 to allow the attachment of cells.…”

Section: Methodsmentioning

confidence: 99%

Anticancer Properties of Plectranthus ornatus-Derived Phytochemicals Inducing Apoptosis via Mitochondrial Pathway

Sitarek

Synowiec

Kowalczyk

et al. 2022

IJMS

Self Cite

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Since cancer treatment by radio- and chemotherapy has been linked to safety concerns, there is a need for new and alternative anticancer drugs; as such, compounds isolated from plants represent promising candidates. The current study investigates the anticancer features of halimane (11R*,13E)-11-acetoxyhalima-5,13-dien-15-oic acid (HAL) and the labdane diterpenes 1α,6β-diacetoxy-8α,13R*-epoxy-14-labden-11-one (PLEC) and forskolin-like 1:1 mixture of 1,6-di-O-acetylforskolin and 1,6-di-O-acetyl-9-deoxyforskolin (MRC) isolated from Plectranthus ornatus in MCF7 and FaDu cancer cell lines. Cytotoxicity was assessed by MTT assay, ROS production by Di-chloro-dihydro-fluorescein diacetate assay (DCFH) or Red Mitochondrial Superoxide Indicator (MitoSOX) and Mitochondrial Membrane Potential (MMP) by fluorescent probe JC-1 (5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide). In addition, the relative amounts of mitochondrial DNA (mtDNA) were determined using quantitative Real-Time-PCR (qRT-PCR) and damage to mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) by semi-long run quantitative Real-Time-PCR (SLR-qRT-PCR). Gene expression was determined using Reverse-Transcription-qPCR. Caspase-3/7 activity by fluorescence was assessed. Assessment of General In Vivo Toxicity has been determined by Brine Shrimp Lethality Bioassay. The studied HAL and PLEC were found to have a cytotoxic effect in MCF7 with IC50 = 13.61 µg/mL and IC50 = 17.49 µg/mL and in FaDu with IC50 = 15.12 µg/mL and IC50 = 32.66 µg/mL cancer cell lines. In the two tested cancer cell lines, the phytochemicals increased ROS production and mitochondrial damage in the ND1 and ND5 gene regions and reduced MMP (ΔΨm) and mitochondrial copy numbers. They also changed the expression of pro- and anti-apoptotic genes (Bax, Bcl-2, TP53, Cas-3, Cas-8, Cas-9, Apaf-1 and MCL-1). Studies demonstrated increase in caspase 3/7 activity in tested cancer cell lines. In addition, we showed no toxic effect in in vivo test for the compounds tested. The potential mechanism of action may have been associated with the induction of apoptosis in MCF7 and FaDu cancer cells via the mitochondrial pathway; however, further in vivo research is needed to understand the mechanisms of action and potential of these compounds.

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Ethylene glycol dimethacrylate and diethylene glycol dimethacrylate exhibits cytotoxic and genotoxic effect on human gingival fibroblasts via induction of reactive oxygen species

Cited by 7 publications

References 32 publications

In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes

In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes

Performance of Fabrics with 3D-Printed Photosensitive Acrylic Resin on the Surface

Anticancer Properties of Plectranthus ornatus-Derived Phytochemicals Inducing Apoptosis via Mitochondrial Pathway

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