2016
DOI: 10.1128/jb.00942-15
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Ethylene Glycol Metabolism in the Acetogen Acetobacterium woodii

Abstract: The acetogenic bacterium Acetobacterium woodii is able to grow by the oxidation of diols, such as 1,2-propanediol, 2,3-butanediol, or ethylene glycol. Recent analyses demonstrated fundamentally different ways for oxidation of 1,2-propanediol and 2,3-butanediol. Here, we analyzed the metabolism of ethylene glycol. Our data demonstrate that ethylene glycol is dehydrated to acetaldehyde, which is then disproportionated to ethanol and acetyl coenzyme A (acetyl-CoA). The latter is further converted to acetate, and … Show more

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Cited by 70 publications
(70 citation statements)
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“…At around 47.5 h, ethanol disappeared although acetaldehyde was still present and acetate was formed up to 5.3 ± 0.02 mM. This indicates that A. woodii oxidizes the ethanol produced to acetate in a second stage of growth, as observed before during growth on ethylene glycol (Trifunović et al ., ). Ethanol oxidation is coupled to the reduction of carbon dioxide in the wood–ljungdahl pathway (WLP) (Bertsch et al ., ).…”
Section: Resultsmentioning
confidence: 97%
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“…At around 47.5 h, ethanol disappeared although acetaldehyde was still present and acetate was formed up to 5.3 ± 0.02 mM. This indicates that A. woodii oxidizes the ethanol produced to acetate in a second stage of growth, as observed before during growth on ethylene glycol (Trifunović et al ., ). Ethanol oxidation is coupled to the reduction of carbon dioxide in the wood–ljungdahl pathway (WLP) (Bertsch et al ., ).…”
Section: Resultsmentioning
confidence: 97%
“…B. Concentrations of acetaldehyde (▲), acetate (●) and ethanol (▼) were measured by gas chromatography as described previously (Trifunović et al ., ), OD 600 (■) was determined photometrically. All data points are mean ± SEM; N = 3 independent experiments.…”
Section: Resultsmentioning
confidence: 99%
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“…This is equivalent to 0.83 ATP per mol of acetate, the largest ATP gain reported so far for an acetogenic C 1 substrate and the second largest after fructose (0.3 for H 2 + CO 2 (Schuchmann and Müller, ); 0.25 for lactate (Weghoff et al ., ); 0.37 for ethanol (Bertsch et al ., ); 0.47 for butanediol (Hess et al ., ); 0.62 for ethylene glycol (Trifunovic et al ., ) and 1.4 for fructose (Schuchmann and Müller, )). The high ATP yield is in accordance to high cell yields (MeOH: 10 g/mol acetate; H 2 + CO 2 : 4.2 g/mol acetate; (Bache and Pfennig, ; Tschech and Pfennig, ).…”
Section: Discussionmentioning
confidence: 99%
“…While A. woodii utilizes the Wood-Ljungdahl Pathway (WLP) for autotrophic growth, alternative pathways for carbon utilization are operative. Alternative pathways include glucose metabolism, 1,2-propanediol degradation, 2,3-butanediol oxidation, ethanol oxidation, caffeate reduction, ethylene glycol metabolism, and alanine metabolism [26][27][28][29][30][31].…”
Section: Introductionmentioning
confidence: 99%