Ethylene-Induced Polyamine Accumulation in Rice (<i>Oryza sativa</i> L.) Coleoptiles

Lee, Tse‐Min; Cai, Chun

doi:10.1104/pp.100.1.238

Cited by 44 publications

(35 citation statements)

References 28 publications

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“…Data are given relative to total RNA, with average values 6 SE shown (n = 3). under anaerobic conditions (Lee and Chu, 1992). Also, Pro, which is synthesized from Orn, is more than 5-fold higher in abundance in anaerobic-than aerobicgerminated rice embryos .…”

Section: Discussionmentioning

confidence: 92%

Analysis of the Rice Mitochondrial Carrier Family Reveals Anaerobic Accumulation of a Basic Amino Acid Carrier Involved in Arginine Metabolism during Seed Germination

et al. 2010

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Given the substantial changes in mitochondrial gene expression, the mitochondrial proteome, and respiratory function during rice (Oryza sativa) germination under anaerobic and aerobic conditions, we have attempted to identify changes in mitochondrial membrane transport capacity during these processes. We have assembled a preliminary rice mitochondrial carrier gene family of 50 members, defined its orthology to carriers of known function, and observed significant changes in microarray expression data for these rice genes during germination under aerobic and anaerobic conditions and across rice development. To determine if these transcript changes reflect alteration of the carrier profile itself and to determine which members of the family encode the major mitochondrial carrier proteins, we analyzed mitochondrial integral membrane protein preparations using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and peptide mass spectrometry, identifying seven distinct carrier proteins. We have used mass spectrometry-based quantitative approaches to compare the abundance of these carriers between mitochondria from dry seeds and those from aerobic-or anaerobic-germinated seeds. We highlight an anaerobicenhanced basic amino acid carrier and show concomitant increases in mitochondrial arginase and the abundance of arginine and ornithine in anaerobic-germinated seeds, consistent with an anaerobic role of this mitochondria carrier. The potential role of this carrier in facilitating mitochondrial involvement in arginine metabolism and the plant urea cycle during the growth of rice coleoptiles and early seed nitrate assimilation under anaerobic conditions are discussed.

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Section: Discussionmentioning

confidence: 92%

Analysis of the Rice Mitochondrial Carrier Family Reveals Anaerobic Accumulation of a Basic Amino Acid Carrier Involved in Arginine Metabolism during Seed Germination

et al. 2010

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“…The diamine putrescine (put), and the PAs spermidine (spd) and spermine (spm) are common amines found in animals or plants. Their ubiquitous nature has been well reported before (Tabor and Tabor, 1984;Lee and Chu, 1992;Altman and Levin, 1993).…”

Section: Introductionmentioning

confidence: 99%

Polyamine levels in the seagrass Cymodocea nodosa

2000

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“…The fluorometric method was adapted for determination of GUS activities by kinetic analysis at 37% in a computer-directed microtiter plate reader (Fluoroscan II; Labsystems, Helsinki, Finland) (Breyne et al, 1993 In vivo ACC oxidase assays were done according to the methods of Hamilton et al (1991) and Lee and Chu (1992) with slight modifications. Either complete 3-week-old Arabidopsis seedlings of which r& had been removed or detached young or mature rosette leaves of a bolting plant (C24 ecotype) were submerged in 3 mL of a 10 mM 24N-rnorpho1ino)ethanesulfonic acid, pH 6.5, solution containing 10 mM ACC and 10 mM aminooxyacetic acid in a 1 0 " flaskand then vacuum infiltrated (10 min undervacuum).…”

Section: Constructsmentioning

confidence: 99%

The Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development.

et al. 1993

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The temporal and spatlal expression of one member of the Arabidopsis 1-aminocyclopropane-1-carboxylate (ACC) synthase gene family (ACS7) was analyzed using a promoter-P-glucuronidase fusion. The expression of ACSl is under developmental control both in shoot and root. High expression was observed in young tissues and was switched off in mature tissues. ACS7 pmmoter activity was strongly correlated with lateral root formation. Dark-grown seedlings exhibited a different expression pattern from light-grown ones. The ACC content and the in vivo activity of ACC oxidase were determined. ACC content correlated with ACS7 gene activity. ACC oxidase activity was demonstrated in young Arabidopsis seedlings. Thus, the ACC formed can be converted into ethylene. In addition, ethylene production of immature leaves was fourfold higher compared to that of mature leaves. The possible involvement of ACSl in influencing plant growth and development is discussed. INTRODUCTIONEthylene is involved in several aspects of plant development from germination and seedling growth to flowering, fruit ripening, leaf abscission, and organ senescence. It also plays a key role in the response to environmental factors (Abeles, 1973; Yang and Hoffman, 1984; Moore, 1989; Van Der Straeten and Van Montagu, 1991).With the recent cloning of genes encoding the two most important enzymes in the biosynthesis of ethylene, l-aminocyclopropane-1-carboxylate (ACC) synthase (Nakajima et al., 1990; Van Der Straeten et al., 1990; Dong et al., 1991;Huang et al., 1991; Olson et al., 1991; and ACC oxidase (ethylene-forming enzyme or EFE) (Hamilton et al., 1990; Spanu et al., 1991;Wang and Woodson, 1991), more information about the molecular regulation of the synthesis of this hormone has been made available. The main focus has been on the regulation of ACC synthase gene expression because of its key regulatory role in the pathway. This implies that diverse inducers of ethylene production are also inducers of de novo synthesis of ACC synthase (Yang and Hoffmann, 1984; Van Der Straeten and Van Montagu, 1991).ACC synthases have been cloned from tomato (Van Der Straeten et al., 1990; Olson et al., 1991; To whom correspondence should be addressed.Yip et al., 1992), winter squash (Nakajima et al., 1990), zucchini (Huang et al., 1991), Arabidopsis (Liang et al., 1992; Van Der Straeten et al., 1992), mung bean (Botellaet al., 1992; Kim et al., 1992), carnation (Park et al., 1992), orchid (ONeill et al., 1993), tobacco (Bailey et al., 1992), and apple (Dong et ai., 1991; Kim et al., 1992). In several cases, it has been shown that ACC synthase genes belong to a multigene family and that they are differentially responsive to various ethyleneinducing factors and conditions such as wounding, fruit ripening, and auxins, although in certain cases some leve1 of coordination was demonstrated (Nakajima et al., 1990; Van Der Straeten et al., 1990; Dong et al., 1991; Olson et al., 1991; ; Yip et ai., 1992). These studies have provided information about general aspects of AC...

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Ethylene-Induced Polyamine Accumulation in Rice (Oryza sativa L.) Coleoptiles

Cited by 44 publications

References 28 publications

Analysis of the Rice Mitochondrial Carrier Family Reveals Anaerobic Accumulation of a Basic Amino Acid Carrier Involved in Arginine Metabolism during Seed Germination

Analysis of the Rice Mitochondrial Carrier Family Reveals Anaerobic Accumulation of a Basic Amino Acid Carrier Involved in Arginine Metabolism during Seed Germination

Polyamine levels in the seagrass Cymodocea nodosa

The Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development.

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