Ethylene Inhibition of Phytochrome-Induced Germination in Potentilla norvegica L. Seeds

Abstract

changes affecting germination when compared to our earlier results (8). Methods used were described previously (8), with the exception that the seeds were planted in 125-ml flasks containing a disc of 5.5 cm, Whatman No. 2 filter paper, and 1.5 ml water. Germination cabinets were held at + 1°C of the indicated temperature. Flasks were sealed with rubber serum stoppers through which an amount of ethylene in I ml of a stock preparation could be injected to achieve the desired concentration. Venting of the flask (to release ethylene) was accomplished by a brief aeration and substitution of a loose-fitting aluminum sheet over the flask mouth in place of the rubber stopper. Typically, seeds were imbibed in darkness for 3 days at 25°C, exposed to unfiltered fluorescent light (60 ,uE m-2 s-') for 12 h in a growth chamber held at 25°C, and germinated in darkness at 25°C for an additional 5 days. Light-proof plywood boxes provided darkness. All manipulations during an experiment were done in a 20°C darkroom provided with a dim-green safelight. An experimental treatment consisted of two flasks of 100 seeds each. Each experiment was repeated one or more times. Germination means of all experiments ± SE are presented. RESULTSThe Pfr-stimulated germination of P. norvegica seeds was reduced about one-half by 0.2 pl/l ethylene supplied continuously from planting (Fig.

Effect of Substrate Dependent Ethylene on Cotton (Gossypium hirsutumL.) at Physiological and Molecular Levels Under Salinity Stress

“…In many species the inhibition of seed germination due to dormancy or stress conditions can be completely or partially reversed by C 2 H 4 or ethephon (Kepczynski and Kepczynska, 1997), which indicates that seeds have a C 2 H 4 response mechanism. Ethylene may be loosely associated with a site required for phytochrome action (Suzuki and Taylorson, 1981). Trujillo et al, (2008) reported that SodERF3, a novel sugarcane ethylene responsive factor (ERF), enhanced salt and drought tolerance when over expressed in tobacco plants.…”

The sweet and musky scent of home: biogenic ethylene fine‐tunes seed germination in wetlands

“…Moreover, ethylene can accelerate germination in cold-stratified seeds of Echinacea angustifolia (Macchia et al 2001). In contrast, seeds of several other wetland plants (Echinochloa crus-galli, Digitaria sanguinalis, Ipomoea purpurea, Persicaria pensylvanica) remained unresponsive to ethylene treatment (Taylorson, 1979), whereas in Chenopodium rubrum, Plantago major, P. maritima and Potentilla norvegica seed germination is inhibited (Olatoye & Hall, 1973;Suzuki & Taylorson, 1981). Yet, although the physiological effects of ethylene on seed germination are well studiedit promotes seed germination through antagonistic interaction with abscisic acid (ABA) and synergistic action with gibberellins (GAs; Ahammed et al 2020)its ecological role as a seed germination trigger is poorly understood.…”

Reversal of ethylene action on cocklebur seed germination in relation to duration of pre‐treatment soaking and temperature

“…The germinatioti of some seeds is stimulated by C2H4 (Toole, Bailey & Toole, 1964;Abeles & Lonski, 1969;Esashi & Leopold, 1969;Egley, 1972;Schonbeck & Egley, 1981). In other seeds, however, G2H4 inhibits germination (Olatoye & Hall, 1972;Suzuki & Taylorson, 1981). An unsolved, important problem is whether such a different mode of action of C2H4 is only determined genetically or can be controlled by conditioning.…”