Ethylene Production by Plant Cell Cultures

Gamborg, O. L.; LaRue, Thomas A.

doi:10.1104/pp.48.4.399

Cited by 38 publications

(7 citation statements)

References 16 publications

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“…The promotion of endogenous ethylene production by soybean cells in the presence of 2,4-D (Table IV) parallels the responses reported for Ruta graveolens and Acer pseudoplatanus cells in culture (3,9). No acetylene-dependent production of ethylene was observed with the auxin and cytokinin levels used in the present study.…”

Section: Methodssupporting

confidence: 88%

Factors Affecting the Reduction of Acetylene by Rhizobium-Soybean Cell Associations in Vitro

Phillips¹

1974

Plant Physiol.

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The acetylene reduction assay was used to measure presumed N-reducing activity in Rhizobium-soybean cell associations in vitro. No acetylene reduction was observed in liquid suspensions of these organisms, but cells plated onto an agar medium from a liquid suspension of Rhizobiumn and soybean cells exhibited acetylene-dependent production of ethylene after 7 to 14 days. Aggregates of soybean cells 0.5 to 2.0 mm in diameter were required for this activity. Decreasing oxygen from 0.20 atm to 0.10, 0.04, or 0.00 atm completely inhibited acetylene reduction. The presence of 2,4-dichlorophenoxyacetic acid or kinetin increased endogenous ethylene production and inhibited acetylene-dependent ethylene production. Acetylene reduction was observed with three out of four strains of R. japonicum tested, and three rhizobial strains, which produce root nodules on cowpeas but not soybeans, formed an association capable of acetylene-dependent ethylene production.

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Section: Methodssupporting

confidence: 88%

Factors Affecting the Reduction of Acetylene by Rhizobium-Soybean Cell Associations in Vitro

Phillips¹

1974

Plant Physiol.

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“…Such a limited biosynthetic capacity may be associated with intensive proliferation of proembryogenic clumps and pre-globular structures. Gamborg and LaRue (1971) postulated that ethylene production was stimulated by 2,4-D only in highly differentiated cells and tissues in contrast to non-differentiated embryogenic tissues (meristematic patterns). It is also known that embryogenic tissues produce less ethylene than non-embryogenic tissues or those with a low regeneration capacity (El Meskaoui and Tremblay 2001;Saly et al 2002).…”

Section: Discussionmentioning

confidence: 97%

“…A similar pattern in auxin-dependent ethylene production was observed in cell suspensions of Ruta graveolens L. and Rosa sp. in which an increase in the 2,4-D concentration resulted in stimulation of ethylene production (Gamborg and LaRue 1971), while lowering of the 2,4-D concentration resulted in decreased ethylene synthesis by the lavender callus (Mensuali-Sodi et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Endogenous ethylene in indirect somatic embryogenesis of Medicago sativa L.

2009

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Ethylene biosynthesis during different phases of somatic embryogenesis in Medicago sativa L. cv. Rangelander using two regeneration protocols, RPI and RPII, was studied. The highest ethylene production was detected during callus growth on induction medium in both regeneration protocols. Significantly less ethylene was produced by embryogenic suspension than by callus (RPII). Developing embryos synthesized higher amounts of ethylene than mature embryos. Production of ethylene was strongly limited by the availability of 1-aminocyclopropane-1-carboxylic acid and also by ACC-oxidase activity. However, removal of ethylene from culture vessels' atmosphere using KMnO 4 or HgClO 4 had no significant effect on callus growth, somatic embryo induction and development. Reducing of ethylene biosynthesis by aminoethoxyvinylglycine substantially decreased somatic embryo production and adversely affected their development, indicating ethylene requirement during proliferation and differentiation but not induction.

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“…inhibitory depending on the type of tissue and physiological process studied (Riov et al 1990, Mattoo andWhite 1991). There are only a few reports of ABA affecting ethylene production in tissue culture (Gamborg and LaRue 1971. Goren et al 1979, Lieberman et al 1979).…”

mentioning

confidence: 99%

ABA promotion of ethylene production in anther culture of Brussels sprouts (Brassica oleracea var. gemmifera) and its relevance to embryogenesis

Biddington¹,

Robinson²,

Lynn³

1993

Physiologia Plantarum

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Abscisic acid (ABA) inhibited embryogenesis in anther culture of Brussels sprouts. This was accompanied by enhanced ethylene production during the first half of the anther culture period followed by a reduction in ethylene during the latter half, when compared to anthers not treated with ABA. The enhancement of ethylene production by ABA 6 h and 48 h after the start of the culture period was counteracted by the ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG). Both AVG and the ethylene antagonist AgNO removed much of the ABA inhibition of embryogenesis, suggesting that at least part of the ABA effect on embryo production is mediated through increased ethylene biosynthesis. ABA promotion of ethylene production was reduced by high temperature: less ethylene evolved from ABA-treated anthers following a 24 h treatment at 35°C than from ABA-treated anthers incubated continuously at 25°C. A high temperature treatment such as this is invariably necessary for embryogenesis in Brussels sprouts anther culture.

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Ethylene Production by Plant Cell Cultures

Cited by 38 publications

References 16 publications

Factors Affecting the Reduction of Acetylene by Rhizobium-Soybean Cell Associations in Vitro

Factors Affecting the Reduction of Acetylene by Rhizobium-Soybean Cell Associations in Vitro

Endogenous ethylene in indirect somatic embryogenesis of Medicago sativa L.

ABA promotion of ethylene production in anther culture of Brussels sprouts (Brassica oleracea var. gemmifera) and its relevance to embryogenesis

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