Ethylene-Responsive Element-Binding Factor 5, ERF5, Is Involved in Chitin-Induced Innate Immunity Response

Son, Geon Hui; Wan, Jinrong; Kim, Hye Jin; Nguyen, Xuan Canh; Chung, Woo Sik; Hong, Jong Chan; Stacey, Gary

doi:10.1094/mpmi-06-11-0165

Cited by 151 publications

(140 citation statements)

References 73 publications

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“…We suggest that ERF5 is a mediator of CAPE1 defense responses because of the GCC box, a cis-acting element found in the promoter of many JA/ethylene-inducible and PR genes. ERF5 was also demonstrated to positively regulate SA signaling and plant immunities against the bacterial pathogen Pst DC3000 and improve plant resistance to pathogens by activating several PR genes (Moffat et al, 2012;Son et al, 2012). In tomato, the overexpression of ERF5 was observed to induce PR genes and conferred tolerance to Ralstonia solanacearum (Li et al, 2011).…”

Section: Discussionmentioning

confidence: 98%

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

et al. 2014

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Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (Solanum lycopersicum) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.

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Section: Discussionmentioning

confidence: 98%

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

et al. 2014

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“…Expression of several of these, including ERF1, ERF5, ERF6, RAP2.2, and ORA59, influences host susceptibility to B. cinerea, with ERF5 a key component of chitinmediated immunity (Berrocal-Lobo et al, 2002;Pré et al, 2008;Moffat et al, 2012;Son et al, 2012;Zhao et al, 2012). Members of the MYB and NAC families (Wang et al, 2009;Ramírez et al, 2011a) have also been shown to influence plant susceptibility to B. cinerea.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis Defense against Botrytis cinerea: Chronology and Regulation Deciphered by High-Resolution Temporal Transcriptomic Analysis

et al. 2012

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Transcriptional reprogramming forms a major part of a plant's response to pathogen infection. Many individual components and pathways operating during plant defense have been identified, but our knowledge of how these different components interact is still rudimentary. We generated a high-resolution time series of gene expression profiles from a single Arabidopsis thaliana leaf during infection by the necrotrophic fungal pathogen Botrytis cinerea. Approximately one-third of the Arabidopsis genome is differentially expressed during the first 48 h after infection, with the majority of changes in gene expression occurring before significant lesion development. We used computational tools to obtain a detailed chronology of the defense response against B. cinerea, highlighting the times at which signaling and metabolic processes change, and identify transcription factor families operating at different times after infection. Motif enrichment and network inference predicted regulatory interactions, and testing of one such prediction identified a role for TGA3 in defense against necrotrophic pathogens. These data provide an unprecedented level of detail about transcriptional changes during a defense response and are suited to systems biology analyses to generate predictive models of the gene regulatory networks mediating the Arabidopsis response to B. cinerea.

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“…These contain an N-terminally located conserved stretch of acidic amino acids (called the 2nd Conserved Motif of group IX [CMIX-2]), which might function as a transcriptional activator domain. The transcriptional activators ERF5 and ERF6 additionally harbor a conserved C-terminal motif (CMIX-5) identified as a putative phosphorylation site by mitogen-activated protein kinases (MPKs), which distinguishes group IXa from group IXb (Fujimoto et al, 2000;Nakano et al, 2006).ERF6 is an activating transcription factor with documented roles in the response to various abiotic and biotic stress conditions, such as oxidative stress (Sewelam et al, 2013;Wang et al, 2013;Vermeirssen et al, 2014), high light (Vogel et al, 2014), cold (Lee et al, 2005;Xin et al, 2007), and biotic stress induced by biotrophic and necrotrophic pathogens (AbuQamar et al, 2006;Dombrecht et al, 2007;Hu et al, 2008;Moffat et al, 2012;Son et al, 2012;Meng et al, 2013). We have previously unraveled the molecular and biological function of ERF6 and its close homolog ERF5 in the mannitol-induced stress response, specifically in actively growing young Arabidopsis leaves (Dubois et al, 2013).…”

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confidence: 99%

The ETHYLENE RESPONSE FACTORs ERF6 and ERF11 Antagonistically Regulate Mannitol-Induced Growth Inhibition in Arabidopsis

et al. 2015

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Leaf growth is a tightly regulated and complex process, which responds in a dynamic manner to changing environmental conditions, but the mechanisms that reduce growth under adverse conditions are rather poorly understood. We previously identified a growth inhibitory pathway regulating leaf growth upon exposure to a low concentration of mannitol and characterized the ETHYLENE RESPONSE FACTOR (ERF)/APETALA2 transcription factor ERF6 as a central activator of both leaf growth inhibition and induction of stress tolerance genes. Here, we describe the role of the transcriptional repressor ERF11 in relation to the ERF6-mediated stress response in Arabidopsis (Arabidopsis thaliana). Using inducible overexpression lines, we show that ERF6 induces the expression of ERF11. ERF11 in turn molecularly counteracts the action of ERF6 and represses at least some of the ERF6-induced genes by directly competing for the target gene promoters. As a phenotypical consequence of the ERF6-ERF11 antagonism, the extreme dwarfism caused by ERF6 overexpression is suppressed by overexpression of ERF11.Together, our data demonstrate that dynamic mechanisms exist to fine-tune the stress response and that ERF11 counteracts ERF6 to maintain a balance between plant growth and stress defense.Plants are constantly challenged to survive and maintain growth in changing environments. In natural environments, as well as in laboratories, growth conditions are rarely optimal, generating a weak but continuous stress. In such suboptimal conditions, the equilibrium between sustained plant growth and activation of stress defense mechanisms is defied and needs to be continuously rebalanced and fine-tuned (Claeys and Inzé, 2013).To unravel these growth-and defense-related mechanisms in Arabidopsis (Arabidopsis thaliana), researchers commonly use in vitro setups in which different growth inhibitory compounds are added to the growth medium (Verslues et al., 2006;Lawlor, 2013;Claeys et al., 2014). Mannitol, for example, is a frequently applied compound to induce mild stress because it results in both inhibition of leaf growth and activation of stress-responsive genes (Kreps et al., 2002;Skirycz et al., 2010Skirycz et al., , 2011Dubois et al., 2013;Claeys et al., 2014;Trontin et al., 2014). Two putative receptorlike kinases, ENHANCED GROWTH ON MANNITOL1 (EGM1) and EGM2, are presumably involved in the detection of mannitol and further downstream activation of the growth and tolerance responses (Trontin et al., 2014). Previously, we have shown that mannitolinduced responses are specific to the different stages of Arabidopsis leaf development (Skirycz et al., 2010). In very young Arabidopsis leaves, in which cells are not yet expanding but still actively dividing, exposure to mannitol triggers the accumulation of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and the transcriptional induction of ethylene-related genes. Interestingly, these responses are extremely fast, with several ETHYLENE RESPONSE FACTORs (ERFs; ERF1, ERF2, ERF5, ERF6, and ERF11...

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Ethylene-Responsive Element-Binding Factor 5, ERF5, Is Involved in Chitin-Induced Innate Immunity Response

Cited by 151 publications

References 73 publications

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

Arabidopsis Defense against Botrytis cinerea: Chronology and Regulation Deciphered by High-Resolution Temporal Transcriptomic Analysis

The ETHYLENE RESPONSE FACTORs ERF6 and ERF11 Antagonistically Regulate Mannitol-Induced Growth Inhibition in Arabidopsis

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