Ethylenediamine Vs. Koh-Glycol in the Removal of the Organic Matter of Dentin

Ettleman, I; Losee, F.L.; Peckham, S.C.

doi:10.1177/00220345560350061701

Cited by 17 publications

(2 citation statements)

References 6 publications

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“…We have attempted to isolate single mineral lamellae from bone by treating it with ethylene diamine which dissolves only collagen but no other organic molecules [51,52]. We obtained mainly stacks of mineral lamellae as well a small proportion of isolated MLs.…”

Section: Longitudinal Viewsmentioning

confidence: 99%

The Ultrastructure of Bone and Its Relevance to Mechanical Properties

2017

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Bone is a biologically generated composite material comprised of two major structural components: crystals of apatite and collagen fibrils. Computational analysis of the mechanical properties of bone must make assumptions about the geometric and topological relationships between these components. Recent transmission electron microscope (TEM) studies of samples of bone prepared using ion milling methods have revealed important previously unrecognized features in the ultrastructure of bone. These studies show that most of the mineral in bone lies outside the fibrils and is organized into elongated plates 5 nanometers (nm) thick, ∼80 nm wide and hundreds of nm long. These so-called mineral lamellae (MLs) are mosaics of single 5 nm-thick, 20-50 nm wide crystals bonded at their edges. MLs occur either stacked around the 50 nm-diameter collagen fibrils, or in parallel stacks of 5 or more MLs situated between fibrils. About 20% of mineral is in gap zones within the fibrils. MLs are apparently glued together into mechanically coherent stacks which break across the stack rather than delaminating. ML stacks should behave as cohesive units during bone deformation. Finite element computations of mechanical properties of bone show that the model including such features generates greater stiffness and strength than are obtained using conventional models in which most of the mineral, in the form of isolated crystals, is situated inside collagen fibrils.

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Section: Longitudinal Viewsmentioning

confidence: 99%

The Ultrastructure of Bone and Its Relevance to Mechanical Properties

2017

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“…• Similar samples of dentin and of bone were rendered anorganic by extraction with ethylene diamine according to the method of Williams and Irvine [8] and of Peckham, et al [9]. The resultant samples were free of all but a trace of organic matter as determined by fluorescence emission through the courtesy of S. Mancewlcz (of the ADA staff at the National Naval Medical Center).…”

Section: Surface Areas Of Anorganic As Distinguished From Crude Dentimentioning

confidence: 99%

Surface studies of natural and synthetic bone mineral and teeth:

Loebenstein¹

1965

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This investigation is part of the dental research program conducted by the National Bureau of Standards, in cooperation with the Council on Dental Research of the Ameri can Dental Association; the National Institute for Dental Research; the Army Dental Corps; the Aerospace Medical Division, USAF School of Aerospace Medicine; and the Veterans Admini stration. IMPORTANT NOTICE NATIONAL BUREAU OF STAND for use within the Government. Befo and review. For this reason, the pub whole or in part, is not authorized Bureau of Standards, Washington, D. the Report has been specifically prep

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Isolation of calcium-phosphate crystals of bone by non-aqueous methods at low temperature

Kim

Rey

Glimcher

1995

Journal of Bone and Mineral Research

152

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We have developed low temperature nonaqueous solution methods as well as low power plasma ashing for the degradation of the organic matrix of bone power which have permitted us to obtain bone crystals essentially free of organic matrix constituents without any significant change in their composition, overall structure, or internal short-range order. We have also been able to disperse the crystals, which has made it possible to examine the isolated crystals by X-ray diffraction and resolution-enhanced Fourier transform infrared (FTIR) spectroscopy and isolated single crystals by high resolution transmission electron microscopy (TEM) and electron diffraction. TEM of isolated single crystals of fish, chicken, mouse and bovine bone have clearly demonstrated that the crystals are very thin plates. No rod or needle-like crystals were observed in any of the bone samples in the four species studied including the earliest crystals deposited. Although there were variations in the size distribution of the crystals in the different species studied, in general the average crystal dimensions were very similar.

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Ethylenediamine Vs. Koh-Glycol in the Removal of the Organic Matter of Dentin

Cited by 17 publications

References 6 publications

The Ultrastructure of Bone and Its Relevance to Mechanical Properties

The Ultrastructure of Bone and Its Relevance to Mechanical Properties

Surface studies of natural and synthetic bone mineral and teeth:

Isolation of calcium-phosphate crystals of bone by non-aqueous methods at low temperature

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