Ethylparaben induces apoptotic cell death in human placenta BeWo cells via the Caspase-3 pathway

Kim, Mi Jin; Kim, Chulhong; An, Mi-Jin; Lee, Ju‐Hyun; Shin, Geun-Seup; Song, Minseok; Kim, Jung-Woong

doi:10.1080/19768354.2020.1711804

Cited by 16 publications

(8 citation statements)

References 23 publications

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“…Similar observations have been documented in other cell types, such as Vero and MCF-10A cells, 12,22 while propylparaben exposure has also been linked to reduced survival in human placental BeWo cells. 9 These findings underscore a complex relationship among cell type, exposure duration, and hydroxybenzoate concentration, resulting in varied levels of cell inhibition. The effects on cell survival are critical given the pivotal role of trophoblast cells in maintaining the placental barrier.…”

Section: Discussionmentioning

confidence: 89%

“…14 viability of human placental BeWo cells in a dose-dependent manner and induces apoptosis by enhancing caspase-3 activity. 9 Furthermore, it has been observed to impact the viability of mouse oviductal secretory epithelial cells, impeding cell cycle progression and reducing progesterone receptor levels in MOE cells. 15 Our research endeavors to provide a more comprehensive understanding of the effects of propylparaben on the trophoblast at the maternal−fetal interface.…”

Section: Introductionmentioning

confidence: 99%

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Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways

Guo,

Yang,

Zhou

et al. 2024

Environ. Health

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Parabens (PBs), especially propylparaben, commonly used in consumer products, pose environmental and health concerns. This study explored propylparaben's cytotoxicity on HTR-8/SVneo human trophoblast cells, revealing significant dosedependent cytotoxic effects, particularly post 48-h exposure. Elevated propylparaben levels triggered apoptosis, evidenced by increased Bax and activated Caspase-3, and induced the G0/G1 cell cycle arrest. Concurrently, an increase in reactive oxygen species and reduced mitochondrial membrane potential indicated oxidative stress and mitochondrial dysfunction. Although Nacetylcysteine (NAC) treatment reduced oxidative stress, cell invasiveness persisted, suggesting propylparaben might affect cell migration through nonoxidative mechanisms. Integrated transcriptome analysis through RNA sequencing revealed 3488 differentially expressed genes affected by propylparaben, highlighting changes in pathways like apoptosis and cell cycle regulation and identifying seven hub genes as potential biomarkers for pregnancy-related complications. This study comprehensively demonstrates the cytotoxic effects of propylparaben on human trophoblast cells, notably through apoptosis induction and cell cycle disruption, thereby providing crucial insights into its potential risks for reproductive health.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways

Guo,

Yang,

Zhou

et al. 2024

Environ. Health

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“…To measure the mitochondrial activity of HFDP cells, cells were cultured in 96-well plates and treated with WEML. After 24 h, 20 μL MTS solution (Promega, Madison, WI, USA) was added and cells were incubated at 37°C for 1 h (Kim et al 2020 ). Absorbance was measured at 490 nm using a microplate reader (ABS Plus; Molecular Devices, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Mangifera Indica leaf extracts promote hair growth via activation of Wnt signaling pathway in human dermal papilla cells

Jung

Lee

et al. 2022

Animal Cells and Systems

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The crosstalk between androgens and Wnt signaling pathways is critical in the hair growth cycle. Therefore, natural products that target these two pathways for the inhibition of hair loss are sought after. In this study, we investigated the effect of water extracts of Mangifera indica leaves (WEML) on hair growth. WEML treatment significantly reduced the expression levels of both dickkopf-1 ( DKK1 ) and type 2 5α-reductase (SRD5A2) involved in Wnt signal suppression activity and dihydrotestosterone (DHT) synthesis, respectively, in human follicle dermal papilla cells (HFDP). In addition, WEML treatment effectively upregulated Wnt target genes and downregulated DKK 1 expression that was increased by DHT treatment. Degranulation analysis in rat basophilic leukemia mast cell line (RBL-2H3) using β-hexosaminidase release assay confirmed that WEML did not exhibit allergenic activity. Furthermore, hair growth was significantly enhanced in in vivo mice model treated with WEML. These results suggest that M. indica leave extract contains bioactive materials that can be used to treat hair loss.

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“…The fraction of apoptotic cells was determined by flow cytometry. Apoptosis activity (%) was calculated by the percentage of apoptotic cells (annexin V-positive and PI-negative) (Bae et al 2020 ; Kim et al 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Physicochemical and biological similarity assessment of LBAL, a biosimilar to adalimumab reference product (Humira®)

Kwon

Jeong

et al. 2021

Animal Cells and Systems

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LBAL was developed as an adalimumab (Humira®) biosimilar using Chinese hamster ovary cell lines. Comparable quality, safety, and efficacy between a biosimilar and its reference product should be ensured for regulatory approval. Here, we present the results of a comprehensive physicochemical and biological characterization between LBAL and Humira®. As physicochemical attributes, primary and higher-order structure, N-glycan profile, and disulfide linkage were investigated. Biological attributes were evaluated by target/receptor binding analysis and in vitro/ex vivo cell-based assays, which are linked to mechanisms of action. As a result, LBAL had the identical amino acid sequence, similar post-translational modifications and N-/C-terminal variants, and comparable primary, secondary, and tertiary structures and disulfide linkage profile. However, some differences in N-glycan profiles were observed. Biological activities, including tumor necrosis factor (TNF) binding, TNF-neutralization, apoptosis, Fc receptor binding, and complementdependent cytotoxicity, were largely consistent. Despite a slightly lower antibody-dependent cellular cytotoxicity activity in LBAL, this difference was not significant under physiological conditions. As indicated, this extensive analytical characterization and functional comparison assessment showed that LBAL was similar to Humira®, with minor differences of no clinical relevance. Taken together, our comparative assessment of physicochemical and biological attributes demonstrated that LBAL is structurally and functionally very similar to Humira®, supporting the biosimilarity of clinical efficacy and safety.

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Ethylparaben induces apoptotic cell death in human placenta BeWo cells via the Caspase-3 pathway

Cited by 16 publications

References 23 publications

Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways

Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways

Mangifera Indica leaf extracts promote hair growth via activation of Wnt signaling pathway in human dermal papilla cells

Physicochemical and biological similarity assessment of LBAL, a biosimilar to adalimumab reference product (Humira®)

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