Etiological and Molecular Biological Investigation of Caprine Herpesvirus 1 Isolated in Bulgaria

Abstract

From goat and bucks with genital disorders and abortions the attempts for isolation of caprine herpesvirus 1 (CHV 1) were carried out. For virus exaltation Dexametazone was used. For viruses isolation vaginal, nasal, rectal, preputial swabs and organ samples were used. Primary and continuous cell cultures rabbit kidney (RK), Madin Darby bovine kidney (MDBK), and embrional bovine trachea (EBTR) were used for cultivation. For determination of DNA type and lipid envelop 60 μg/ml iod desoxiuridine (JDUR) and the ether treat ment was used. Neutralization by specific hyperimmune serum obtained from Switzerland was performed. Five CHV 1 strains were isolated by cell cultures and identified as goat herpesviruses from dif ferent Bulgarian regions. After electron microscopy the viral agents with typical size and morphology for herpesviruses were established. For demonstration gC gene of CHV 1 the polymerase chain reaction (PCR) with primers designed from sequences deposited in gene bank were developed. Isolated on cell cultures herpesviruses were proved as caprine herpesvirus 1 by using applied PCR variant. The products after gC gene amplification from Bulgarian isolates were separated on the same place as the amplicons of ref er ence CHV 1 strains.