Etiological Role of Sulfate-Reducing Bacteria in the Development of Inflammatory Bowel Diseases and Ulcerative Colitis

Abstract

Inflammatory bowel disease and ulcerative colitis are complex multifactorial diseases of unknown etiology. The etiological role of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 in the development of these inflammatory diseases in rats was studied. Applying sulfate-reducing bacteria, ulcerative colitis in the animals was experimentally induced at the fist time. In total 45 animals in three groups were separated and used in this experiment. Animals in the first group received the standard diet containing a special certified feed for rats and were used as a control. The second group received the standard feed and a dose (1 ml per each day) of modified Kravtsov-Sorokin's liquid medium for the initiation of own potential animal intestinal microflora of sulfate-reducing bacteria. The third group of the animals received the standard feed and dose of a suspension with D. piger Vib-7 and Desulfomicrobium sp. Rod-9 (at a ratio of 1 to 1) in the medium. The changes in the colonic microbiota under these conditions were studied. The bacteria belonging to the normal colonic microbiota were associated with the etiology of inflammatory bowel disease and ulcerative colitis. The concentration of sulfide and acetate in feces from different sections of the large intestine of the rats was determined. The level of ulcerations in the second and third groups of sick animals under the specific conditions was demonstrated. The described experimental results could be particularly useful for the study of inflammatory bowel disease and ulcerative colitis and its therapeutic strategy. These data are also indispensable to apply into mechanistic details that will facilitate better preclinical drug/therapy design to target specific components involved in the disease pathogenesis. Keywords

Model-based Characterization of the Parameters of Dissimilatory Sulfate Reduction Under the Effect of Different Initial Density of Desulfovibrio piger Vib-7 Bacterial Cells

“…Taking into consideration all the obtained RESULTS, it should be noted that the logistic sigmoid curves are widely used to describe various processes of bacterial growth [ 15 - 21 , 28 ], which are supported by the RESULTS of these studies. The mathematical model of the D. piger Vib-7 intensity growth and the sulfate reduction process, which was described, can help provide a more detailed understanding of the etiological role of sulfate-reducing bacteria in the development of inflammatory human bowel processes and diseases [ 29 ].…”

Dissimilatory sulfate reduction in the intestinal sulfate-reducing bacteria

“…The sulfate dissimilation process is called the "dissimilatory sulfate reduction" or "sulfate respiration" [54]. Intensive sulfate reduction by SRB, and accordingly the accumulation of toxic hydrogen sulfide in the intestine, is leading to the development of various diseases [12,16,46,49,55]. SRB and the products of their metabolism are often found during bloody diarrhea and abdominal pain [51,55].…”

“…SRB and the products of their metabolism are often found during bloody diarrhea and abdominal pain [51,55]. It is believed that they can cause weight loss, frequent defecation, arthritis, rheumatic diseases, increased intestinal permeability, ulcerative colitis, and malaise, in general [12,13,15,17,46,49,55].…”

“…Intestinal sulfate-reducing bacteria and the bowel diseases. Sulfate-reducing bacteria Desulfotomaculum, Desulfobulbus, Desulfomicrobium, Desulfomonas, and Desulfovibrio genera belong to the intestinal microbiocenosis in humans and animals [1,22,23,24,46]. The knowledge on the interaction of SRB with other microorganisms in intestines is not sufficient.…”

“…Similarly to some methanogens, they can cause the development of other diseases, including cholecystitis, abscesses of the brain and abdomen, ulcerative enterocolitis, cancer, etc. [1,20,46,49].…”