Etiology of acute diarrhea among children in Ribeirão Preto-SP, Brazil

Medeiros, Marta Inês Cazentini; Neme, Suzel Nogueira; Silva, Paulo da; Capuano, Divani Maria; Errera, Maria Clarice; Fernandes, Sueli Aparecida; Valle, Glória Reginado; Ávila, Fernando Antônio de

doi:10.1590/s0036-46652001000100004

Cited by 42 publications

(40 citation statements)

References 11 publications

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“…This is in contrast with studies from other places in Brazil, which detected S. sonnei as the most frequent serogroups: Ribeirão Preto-SP (Medeiros et al 2001), and Salvador-BA (Diniz- Santos et al 2005). However, our finding is consistent with other reports from Northeastern Brazil (Lima et al 1995), Porto Alegre-RS (Santos et al 1997), and São José do Rio Preto-SP (Almeida et al 1998), and those from other Latin America developing countries such as Argentina (Merino et al 2004), Peru (Jones et al 2004), and Chile (Fulla et al 2005).…”

Section: Discussioncontrasting

confidence: 99%

Frequency of serovars and antimicrobial resistance in Shigella spp. from Brazil

Peirano

Souza

Rodrigues

2006

Mem. Inst. Oswaldo Cruz

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Section: Discussioncontrasting

confidence: 99%

Frequency of serovars and antimicrobial resistance in Shigella spp. from Brazil

Peirano

Souza

Rodrigues

2006

Mem. Inst. Oswaldo Cruz

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“…Estudos realizados em diferentes estados no Brasil têm demonstrado uma prevalência de Cryptosporidium spp em crianças (0 a 12 anos de idade) de 4,2%, 3,3% e 1,8% em Uberlândia (MG) 11 , Rio de Janeiro (RJ) 30 e Ribeirão Preto (SP) 22 , respectivamente.…”

Section: Discussionunclassified

Presença de Cryptosporidium spp em crianças com diarréia aguda em uma creche pública de Recife, Estado de Pernambuco

Nascimento

Cavalcanti

Irmão

et al. 2009

Rev. Soc. Bras. Med. Trop.

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Indivíduos imunossuprimidos estão sujeitos a uma série de complicações infecciosas causadas por agentes oportunistas, e em decorrência deste fato, poder-se-á observar o aparecimento de algumas doenças denominadas emergentes 20 ABSTRACTThe objective of the present study was to analyze the frequency of oocysts of Cryptosporidium spp in fecal samples from children aged one to fourteen years at a public daycare center located in a needy community in the city of Recife, Pernambuco. The investigation was carried out between June 28, 2006, and April 3, 2007, and involved 182 children. Among the samples analyzed, 59 (32.4%) were positive regarding the presence of oocysts of Cryptosporidium spp, and the age group most affected was between three and five years (54.2%). The high frequency of samples positive for Cryptosporidium spp obtained in this study confirms that daycare centers are an environment that favors such occurrences, because of the direct contact between children or between children and staff. The most important infection route for Cryptosporidium spp is person-to-person transmission, which is well illustrated in daycare centers. Immaturity, deficiencies of the immune system and inadequate hygiene habits are factors that also contribute towards this type of infection.

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“…19,20,[22][23][24][25][26][27][28][29][30]32,33 Despite the number of reports indicating the association of this parasite with diarrhea in different settings and the presence of this parasite in the environment in different regions of this country, only 1 molecular assessment has been previously performed, in clinical samples collected in Fortaleza, Brazil. 47 We used previously described molecular tools 39,41,42 to analyze samples collected from children attending a day care at the Hospital das Clínicas, from March to May 2001, during which an outbreak of cryptosporidiosis took place.…”

Section: Discussionmentioning

confidence: 99%

“…Studies conducted in different regions of Brazil have shown that Cryptosporidium sp is associated with symptomatic intestinal disease in HIV infected individuals and in children suffering of acute diarrhea, including those attending day care. [22][23][24][25][26][27][28] Environmental contamination with Cryptosporidium oocysts has been observed in rivers and public water reservoirs serving different regions in Sao Paulo. [29][30][31][32] Nevertheless, no massive cryptosporidiosis outbreaks associated with consumption of water in these regions have been reported.…”

Section: Introductionmentioning

confidence: 99%

Multilocus Genotyping of Cryptosporidium Hominis Associated With Diarrhea Outbreak in a Day Care Unit in São Paulo

Gonçalves

Silva²,

Eduardo³

et al. 2006

Clinics

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Gonçalves EM do N, da Silva AJ, Eduardo MB de P, Uemura IH, Moura INS, Castilho VLP et al. Multilocus genotyping of Cryptosporidium hominis associated with diarrhea outbreak in a day care unit in São Paulo. Clinics. 2006;61(2):119-26.A number of species of Cryptosporidium are associated with diarrhea worldwide. Little data exists regarding the genotypes and species of Cryptosporidium associated with cases of infections in Brazil. PURPOSE: In the present study, we ascertained by molecular methods the species and the genotype of Cryptosporidium sp from a diarrhea outbreak diagnosed in a day care at the Hospital Clínicas, São Paulo University Medical School. MATERIALS AND METHODS: Specific identification and typing of the isolates associated with the outbreak was done by DNA sequencing analysis of fragments amplified by polymerase chain reaction (PCR) from 3 different Cryptosporidium loci: the SSUrRNA coding region, the Cryptosporidium oocyst wall protein (COWP) gene, and the microsatellite locus 1 (ML1), a tandem GAG-trinucleotide repeat containing substitutions that differentiate the genotypes of Cryptosporidium parvum and Cryptosporidium hominis. RESULTS: A total of 29 positive samples from the outbreak were studied by the molecular methods described. Our study revealed the presence of a single genotype of Cryptosporidium hominis in all samples. CONCLUSION: The molecular analysis reinforced the hypothesis that the transmission of Cryptosporidium hominis during the period the samples were collected occurred in an outbreak pattern, possibly by person-to-person contact through the fecal-oral route. As far as we know, this is the first time that molecular tools have been used to identify the species and the genotype of isolates showing the presence of the ML1 genotype in samples from Brazilian patients.

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Etiology of acute diarrhea among children in Ribeirão Preto-SP, Brazil

Cited by 42 publications

References 11 publications

Frequency of serovars and antimicrobial resistance in Shigella spp. from Brazil

Frequency of serovars and antimicrobial resistance in Shigella spp. from Brazil

Presença de Cryptosporidium spp em crianças com diarréia aguda em uma creche pública de Recife, Estado de Pernambuco

Multilocus Genotyping of Cryptosporidium Hominis Associated With Diarrhea Outbreak in a Day Care Unit in São Paulo

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