Etiology of Acute Respiratory Infections in Infants

Kumar, Prawin; Medigeshi, Guruprasad R.; Mishra, Vishnu S.; Islam, Mojahidul; Randev, Shivani; Mukherjee, Aparna; Chaudhry, Rama; Kapil, Arti; Jat, Kana Ram; Lodha, Rakesh; Kabra, S. K.

doi:10.1097/inf.0000000000001359

Cited by 32 publications

(18 citation statements)

References 34 publications

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“…(a) Multiplexing and the utility of identifying mixed infections. Multiplexing of molecular assays can facilitate identification of mixed viral infections (268)(269)(270). Coinfections are defined as the detection of more than one virus in a patient specimen.…”

Section: Molecular Panel Testing For Respiratory Viruses (I) Defininmentioning

confidence: 99%

“…For example, in Japan, a recent study found that 43.8% of patients who were diagnosed with a CoV infection were also infected with an additional virus (273). In another study, coinfections of two or more viruses were identified in approximately 18% of infants with an acute respiratory illness; RV was the most common coinfecting virus, but other viruses, such ADV, hMPV, and PIVs, were also codetected (270). Thus, the impact of mixed infection on patient outcomes is still under debate.…”

Section: Molecular Panel Testing For Respiratory Viruses (I) Defininmentioning

confidence: 99%

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Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

et al. 2018

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SUMMARYRespiratory viral infections are associated with a wide range of acute syndromes and infectious disease processes in children and adults worldwide. Many viruses are implicated in these infections, and these viruses are spread largely via respiratory means between humans but also occasionally from animals to humans. This article is an American Society for Microbiology (ASM)-sponsored Practical Guidance for Clinical Microbiology (PGCM) document identifying best practices for diagnosis and characterization of viruses that cause acute respiratory infections and replaces the most recent prior version of the ASM-sponsored Cumitech 21 document,Laboratory Diagnosis of Viral Respiratory Disease, published in 1986. The scope of the original document was quite broad, with an emphasis on clinical diagnosis of a wide variety of infectious agents and laboratory focus on antigen detection and viral culture. The new PGCM document is designed to be used by laboratorians in a wide variety of diagnostic and public health microbiology/virology laboratory settings worldwide. The article provides guidance to a rapidly changing field of diagnostics and outlines the epidemiology and clinical impact of acute respiratory viral infections, including preferred methods of specimen collection and current methods for diagnosis and characterization of viral pathogens causing acute respiratory tract infections. Compared to the case in 1986, molecular techniques are now the preferred diagnostic approaches for the detection of acute respiratory viruses, and they allow for automation, high-throughput workflows, and near-patient testing. These changes require quality assurance programs to prevent laboratory contamination as well as strong preanalytical screening approaches to utilize laboratory resources appropriately. Appropriate guidance from laboratorians to stakeholders will allow for appropriate specimen collection, as well as correct test ordering that will quickly identify highly transmissible emerging pathogens.

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Section: Molecular Panel Testing For Respiratory Viruses (I) Defininmentioning

confidence: 99%

Section: Molecular Panel Testing For Respiratory Viruses (I) Defininmentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

et al. 2018

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“…Bakteri adalah penyebab utama dan umum yang bertanggungjawab atas infeksi saluran pernapasan bawah, seperti Streptococcus pneumoniae. Ancaman ISPA juga dapat disebabkan oleh virus (syncytialvirus (RSV), human metapneumovirus (HMPV), coronavirus, enterovirus/ rhinovirus, virus influenza, virus parainfluenza, adenovirus) yang mudah bermutasi dan dapat menjadi ancaman serius yang menimbulkan epidemi maupun pandemi (Kumar et al, 2017;Naz et al, 2019). Terkadang pada beberapa kasus kejadian ISPA pada anak adalah koinfeksi virus atau bakteri atipikal yang dapat menimbulkan gejala yang lebih berat (El Baroudy et al, 2018).…”

Section: A Pendahuluanunclassified

Health Education on Acute Respiratory Syndrome and Uric Acid Testing for Community in Gunungsari District, Pemijahan, Bogor County

Hikmah

Ariningpraja

Fadilla

et al. 2021

CJPM

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Efforts to prevent acute respiratory infections (ARI) are very important, because of the ease of transmission and can cause death. Health data from the Desa Gunung Sari Health Center, Kecamatan Pamijahan, Kabupaten Bogor in 2017 indicated that ARI was the most frequent complaint. The purpose of this community service is to educate health cadres about ARI. In addition, an examination of uric acid levels was held for the people of Desa Gunung Sari which was a series of counseling activities. The method used is counseling and direct examination. The counseling participants were 45 people and the uric acid examination was 146 people. The results showed that the cadres listened well to the counseling material, as seen in the correct answers to spontaneous questions during the counseling. The community's uric acid levels were mostly in the normal category and education helps increase public understanding of ARI. Thus, the continuity of similar activities is very much needed.

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“…The study was approved by the ethics committee of THSTI THS 1.8.1/(91) dated 13 April 2020. RNA samples from other respiratory virus infections were used from a study described previously (Kumar et al, 2017), which was a cohort of newborns who were followed for the first year of life, for each episode of acute respiratory infection. Nasopharyngeal aspirates were collected to identify the causative respiratory viruses using multiplex real-time PCR assay.…”

Section: Ethicsmentioning

confidence: 99%

“…To ascertain the selectivity of the assay, the stored RNA samples isolated from patients positive for other respiratory viruses, namely, parainfluenza virus (n = 1), influenza A virus (n = 2), human adenovirus (n = 1), rhinovirus (n = 4), human coronavirus OC43 (n = 1), and human respiratory syncytial virus (n = 1) were tested (Kumar et al, 2017).…”

Section: Assessment Of Cross-reactivitymentioning

confidence: 99%

Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor

Anantharaj

Das

Sharanabasava

et al. 2020

Front. Mol. Biosci.

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The gold standard for the diagnosis of SARS-CoV-2, the causative agent of COVID-19, is real-time polymerase chain reaction (PCR), which is labor-intensive, expensive, and not widely available in resource-poor settings. Therefore, it is imperative to develop novel, accurate, affordable, and easily accessible assays/sensors to diagnose and isolate COVID-19 cases. To address this unmet need, we utilized the catalytic potential of peroxidase-like DNAzyme and developed a simple visual detection assay for SARS-CoV-2 RNA using a conventional thermal cycler by the PCR-induced generation of DNAzyme sensor. The performance of RT-PCR DNAzyme-based sensor was comparable to that of real-time PCR. The pilot scale validation of RT-PCR DNAzyme-based sensor has shown ~100% sensitivity and specificity in clinical specimens (nasopharyngeal swab, n = 34), with a good correlation (Spearman r = 0.799) with the Ct-value of fluorescence probe-based real-time PCR. These findings clearly indicate the potential of this inexpensive, sensitive, and specific molecular diagnostic test to extend our testing capabilities for the detection of SARS-CoV-2 to curtail COVID-19 transmission.

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Etiology of Acute Respiratory Infections in Infants

Abstract: In this cohort of infants, ARI incidence was 1.8 episodes per year per infant; 95% were upper respiratory tract infections. Viruses were identified in 63.3% episodes, and the most common viruses detected were rhinovirus, respiratory syncytial virus and parainfluenza virus.

Cited by 32 publications

References 34 publications

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

Health Education on Acute Respiratory Syndrome and Uric Acid Testing for Community in Gunungsari District, Pemijahan, Bogor County

Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor

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