Etmopterus lantern sharks use coelenterazine as the substrate for their luciferin-luciferase bioluminescence system

Mizuno, Gaku; Yano, Daichi; Paitio, José; Endo, Hiromitsu; Oba, Yuichi

doi:10.1016/j.bbrc.2021.09.007

Cited by 8 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has traditionally been assumed that lantern shark luciferin, although as yet unidentified, is an endogenous product of shark metabolism. However, Mizuno et al (2021) have recently claimed that lantern shark luciferin is instead derived from the food they consume. This hypothesis is, nevertheless, still under debate, given that coelenterazine, a luciferin that Mizuno et al (2021) believe to be lantern shark luciferin, has hitherto not been detected in lantern shark photophores (Renwart and Mallefet, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…However, Mizuno et al (2021) have recently claimed that lantern shark luciferin is instead derived from the food they consume. This hypothesis is, nevertheless, still under debate, given that coelenterazine, a luciferin that Mizuno et al (2021) believe to be lantern shark luciferin, has hitherto not been detected in lantern shark photophores (Renwart and Mallefet, 2013). As there is no likelihood that lantern shark specimens incubated in an artificial uterus would receive external inputs of coelenterazine, either from environmental fluid or the maternal body, we could establish the validity of Mizuno et al's (2021) hypothesis by (1) confirming whether or not these specimens emit light without an external input of coelenterazine and, if not, (2) confirming whether the oral administration of coelenterazine promotes the subsequent development of bioluminescence.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Five-Month Incubation of Viviparous Deep-Water Shark Embryos in Artificial Uterine Fluid

et al. 2022

View full text Add to dashboard Cite

In this study, we developed an extra-uterine life-support system (artificial uterus) for viviparous elasmobranchs. Using this system, we maintained two embryonic specimens of the slendertail lantern shark (Etmopterus molleri) for approximately 5 months, the longest published record of the captive maintenance of the embryos of this species. This system is characterized by the use of a urea-containing artificial incubation fluid, of which the salinity and osmotic pressure are approximately equivalent to those of shark blood plasma. We hypothesized that this fluid reduces the salinity gradient between embryonic blood plasma and the environmental fluid, which thereby contributes to countering the low osmoregulatory capacity of viviparous shark embryos. However, the incubated specimens subsequently died during the process of seawater adaptation following “artificial birth.” Accordingly, information pertaining to uterine chemical dynamics throughout the gestation period is necessary for the design of a more effective incubation system. This technique will be relevant and may find application in conservation breeding at public aquaria in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Five-Month Incubation of Viviparous Deep-Water Shark Embryos in Artificial Uterine Fluid

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Lantern sharks have been hypothesized to use self-produced luciferin (e.g., [ 8 ]). Recently, Mizuno et al provided another hypothesis regarding the source of the lantern shark luciferin [ 9 ]. The authors claimed that the lantern sharks do not have the ability to produce luciferin but “steal” it from prey.…”

Section: Discussionmentioning

confidence: 99%

Successful delivery of viviparous lantern shark from an artificial uterus and the self-production of lantern shark luciferin

Tomita,

Toda,

Kaneko

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Our recent success in the long-term maintenance of lantern shark embryos in artificial uterine systems has provided a novel option for the medical treatment of premature embryos for captive viviparous elasmobranchs. The remaining issue with this system is that the embryos cannot survive the abrupt change in the chemical environment from artificial uterine fluid (AUF) to seawater during delivery. To overcome this issue, the present study developed a new protocol for seawater adaptation, which is characterized by a long-term and stepwise shift from AUF to seawater prior to delivery. This protocol was employed successfully, and the specimen survived for more than seven months after delivery, the longest captive record of the species. During the experiment, we unexpectedly detected bioluminescence of the embryonic lantern shark in the artificial uterus. This observation indicates that lantern sharks can produce luciferin, a substance for bioluminescence. This contradicts the recent hypothesis that lantern sharks lack the ability to produce luciferin and use luciferin obtained from food sources.

show abstract

“…2021; Mizuno et al . 2021), photophore distribution and its interrelationship with squamation patterns (Hubbs et al . 1967; Reif 1985; Claes & Mallefet 2009 b ; Claes et al .…”

mentioning

confidence: 99%

Illuminating the evolution of bioluminescence in sharks

Ferrón

2023

Palaeontology

View full text Add to dashboard Cite

The evolutionary context in which shark bioluminescence originated is poorly understood, despite it being critical to uncovering influential factors in the evolutionary history and diversity of living chondrichthyans as well as the mechanisms of deep‐water colonization by vertebrates. This study provides the first joint reconstruction of the habitats, lifestyles, and occurrence of bioluminescence in the evolution of squalomorph sharks using ancestral state estimation analysis to resolve the timing of deep‐sea colonization, the evolutionary origin of bioluminescence and the ancestral ecologies of this group. The results suggest that most squalomorphs originated in neritic environments from where they colonized deep waters on several independent occasions during the Late Jurassic and Early Cretaceous, predating most of the previous estimates of the timing of this event. The colonization of the deep sea took place via the benthic zone, in contrast to the view that an intermediate mesopelagic stage occurred during this ecological transition. Finally, the analyses accounting for uncertainty of the presence of bioluminescence strongly support that this trait evolved only once among sharks in a bathydemersal ancestor. This study reveals that shark bioluminescence evolved in a complex scenario that combines elements of several previous proposals, and enriches our perspective on the sequence of events that characterized the vertebrate conquest of the deep sea.

show abstract

Etmopterus lantern sharks use coelenterazine as the substrate for their luciferin-luciferase bioluminescence system

Cited by 8 publications

References 25 publications

Five-Month Incubation of Viviparous Deep-Water Shark Embryos in Artificial Uterine Fluid

Five-Month Incubation of Viviparous Deep-Water Shark Embryos in Artificial Uterine Fluid

Successful delivery of viviparous lantern shark from an artificial uterus and the self-production of lantern shark luciferin

Illuminating the evolution of bioluminescence in sharks

Contact Info

Product

Resources

About