Etude de la glande cémentaire d'un Polychète tubicole (Pectinaria (=lagis) koreni) à l'aide de la microsonde électronique, du microanalyseur ionique et du microscope électronique à balayage

Truchet, M.; Vovelle, Jean

doi:10.1007/bf02223321

Cited by 14 publications

(6 citation statements)

References 2 publications

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“…3 B, C) of both biocements were reflected in the roughness data (see below). The bubbly nature of both biocements was evident in SEM and supported previous SEM analyses in related organisms, Phragmatopoma californica (Stewart et al 2004) and Pectinaria kornei (Truchet & Vovelle 1977;Volvelle 1979). We collected 22 scans of Phragmatopoma lapidosa and 21 scans of Pectinaria gouldii using AFM in tapping mode.…”

Section: Resultssupporting

confidence: 85%

Surface analyses of biocements from Pectinaria gouldii (Polychaeta: Pectinariidae) and Phragmatopoma lapidosa (Polychaeta: Sabellariidae)

et al. 2009

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Pectinaria gouldii and Phragmatopoma lapidosa are marine polychaetes that reside in protective structures built from sand grains bound together using proteinaceous cement secreted from specialized glands. P. gouldii constructs a solitary, ice-cream-cone-like structure. The smaller, gregarious P. lapidosa forms a large, reef-like mound. This study investigates the physical features of these two polychaete biocements, linking structure and function in two marine environments. The surface structures of hydrated biocement samples were analyzed using atomic force microscopy (AFM), and the surface structures and composition of dehydrated biocement samples were analyzed using scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS). Atomic force analyses indicate that (in their native states) the surface roughness, adhesion, and stiffness of P. gouldii biocement are greater than P. lapidosa biocement. The surface of P. gouldii resembled “cottage cheese,” while the surface of P. lapidosa had smoother features. SEM revealed “popped bubble” features that indicated a solid foam-like material for both biocements. EDS confirmed the presence of calcium, magnesium, and phosphorous in both biocements, with varying amounts of these three elements at different locations on the same sample.

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Section: Resultssupporting

confidence: 85%

Surface analyses of biocements from Pectinaria gouldii (Polychaeta: Pectinariidae) and Phragmatopoma lapidosa (Polychaeta: Sabellariidae)

et al. 2009

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“…Mn and Fe are additional minor elements encountered in the cement whose presence is common in Ca-Mg concretions of marine invertebrates (Simkiss, 1983). Our results confirm those of Gruet et al (1987) for S. alveolata, Truchet and Vovelle (1977) and Vovelle and Grasset (1990) for P. koreni, and Lafon (1959) for L. conchilega. Phragmatopoma caudata cement has never been studied before, whereas Phragmatopoma californica has been studied by Stewart et al (2004), Sun et al (2007), and Zhao et al (2005).…”

Section: Discussionsupporting

confidence: 88%

“…Sliced samples previously analyzed with EPMA were observed under back-scattered electron mode. Image resolution is higher under SEM than EPMA, thus allowing characterization of the internal structure of the tube and the observation of grain/cement relationships (Truchet and Vovelle, 1977).…”

Section: Procedures For Scanning Electron Microscopy Analysismentioning

confidence: 99%

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Inter- and intraspecific variability in the chemical composition of the mineral phase of cements from several tube-building polychaetes

Fournier¹,

Étienne²,

Cam³

2010

Geobios

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“…At mass 55+ in a cryofixed mouse liver section the predominant intensity was ascribed to KO+ rather than Mnf. Truchet & Vovelle (1977) have reported the separation of 56Fef from CaOf and MgOz+ in their study of the composition of secretory glands in a polychete (Fig. lo), showing that Fe was a negligible component of the m/e=56+ peak.…”

Section: Mass S P E C T R Amentioning

confidence: 95%

Applications of secondary ion mass spectrometry (SIMS) in biological research: a review

Burns

1982

Journal of Microscopy

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ion mass spectrometry, microanalysis, elemental localization, trace elements, alkaline earths. SUMMARY Secondary ion mass spectrometry (SIMS) is a potentially valuable but not fully exploited technique for problems in biological research. It is valuable because of: (1) detection of all elements and isotopes from mass 1, hydrogen; (2) high sensitivity for physiologically important elements, Na, K, Mg, Ca; (3) ion imaging of elements in areas as large as 250 pm in diameter with a lateral resolution of 0.5 pm; (4) promising efforts at quantitation at levels as low as 0.1 mmollkg; (5) ability to analyse sequential layers to form a three-dimensional analysis. The problems which complicate its use are primarily variations in ion emission, presence of polyatomic interferences and tissue preparation. Examples are cited of SIMS analysis of biomineralization, botany, toxicology and physiology, mostly by ion imaging techniques. SIMS has not yet been fully exploited for any single biological problem. In particular, studies using isotope discrimination, quantitative analysis and depth profiling will enhance the usefulness of SIMS as a technique for biological research. I N T R O D U C T I O NAdvances in biological research have been rapid in the past 30 years due, in part, to our altered conception of the cell. Application of new scientific instrumentation, the electron microscope, to biological research made possible this fundamental conceptual change, opening the way to rapid and revolutionary progress (Kuhn, 1970). We had a view of the variegated intracellular structures and could verify the interfaces between cells. Now the crucial questions of biological research are concerned with the specific sites and extent of cellular interactions. These questions require instrumentation which can identify, measure and localize events at a cellular level.Secondary ion mass spectrometry (SIMS) is widely used for study of elemental localization problems in geochemistry, metallurgy and electronics , and has technical specifications which make it potentially useful in solving problems of physiological importance. The point of this review is to acquaint the reader with SIMS technique, to review the applications of SIMS analysis to biological problems, and to give a considered viewpoint of how SIMS may be useful in future applications. This is not meant to be a comprehensive review, which would be difficult because SIMS is not an indexed key word in biological indexing services. My apologies to authors whose material has not been included. In addition to the references cited in the text, a list

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Etude de la glande cémentaire d'un Polychète tubicole (Pectinaria (=lagis) koreni) à l'aide de la microsonde électronique, du microanalyseur ionique et du microscope électronique à balayage

Cited by 14 publications

References 2 publications

<p class="HeadingRunIn"><strong>Surface analyses of biocements from <em>Pectinaria gouldii</em> (Polychaeta: Pectinariidae) and <em>Phragmatopoma lapidosa </em>(Polychaeta: Sabellariidae)</strong></p>

<p class="HeadingRunIn"><strong>Surface analyses of biocements from <em>Pectinaria gouldii</em> (Polychaeta: Pectinariidae) and <em>Phragmatopoma lapidosa </em>(Polychaeta: Sabellariidae)</strong></p>

Inter- and intraspecific variability in the chemical composition of the mineral phase of cements from several tube-building polychaetes

Applications of secondary ion mass spectrometry (SIMS) in biological research: a review

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