Etude Immunologique de la Prothrombine et de la Thrombine Humaines

Abstract

RésuméDes anticorps de lapin anti-prothrombine humaine sont obtenus par immunisation des animaux avec de faibles quantités de prothrombine purifiée et absorption des immunsérums par du sérum humain dépourvu de prothrombine.Les anticorps antiprothrombine forment avec la prothrombine un précipité spécifique et inhibent son activité biologique. Ils n’ont aucune action vis-à-vis des facteurs VII, IX et X.Les anticorps antiprothrombine humaine réagissent également avec la thrombine humaine. On observe en immunodiffusion une réaction d’identité partielle entre la prothrombine et la thrombine. L’absorption des anticorps par la prothrombine supprime complètement leur activité; par contre, ils conservent leur activité antiprothrombine s’ils sont absorbés par la thrombine. Ces observations indiquent que la molécule de prothrombine perd une partie de son matériel antigénique en se transformant en thrombine.L’immuno-électrophorèse à l’aide d’anticorps antiprothrombine montre que la prothrombine migre en position alpha-2 et que la thrombine a une mobilité moindre en gel d’agarose.Les anticorps antiprothrombine humaine permettent une estimation quantitative de la prothrombine humaine par référence à son activité antigénique. Il apparaît qu’au cours des processus de purification une partie des molécules de prothrombine perdent leur pouvoir de se transformer en thrombine en conservant leurs propriétés antigéniques.

The Purification of Human Factor II (Prothrombin) and the Preparation of a Specific Antiserum

“…This is in contrast to other authors, e.g. Josso et al [9] and Ganrot and Niléhn [6], who had to absorb their anti-factor II with factor II free serum or with the macroglobulin fraction of Sephadex G-200 gel filtration.…”

Congenital dysprothrombinemia: an inherited structural disorder of human prothrombin

“…Thus, one previously reported case of congenital hypoprothrombinemia (8,9) has been found to lack almost completely any material capable of being fractionated by a standard technique for the isolation of plasma prothrombin (10). In two other unrelated cases, the deficiency of plasma prothrombin was equally severe by biological and immunologic assays (5,1). We have studied a large family, in which 11 members have halfnormal levels of prothrombin biological activity.…”

“…The recent development of Received for publication 9 June 1969. methods for the purification of human prothrombin (1)(2)(3) and the availability of specific antisera (4)(5)(6)(7) have permitted the use of physical and immunologic techniques for the investigation of this problem. Thus, one previously reported case of congenital hypoprothrombinemia (8,9) has been found to lack almost completely any material capable of being fractionated by a standard technique for the isolation of plasma prothrombin (10).…”

“…There is no evidence of a relationship between the connective tissue disease and "prothrombin Cardeza," nor could any linkage be established between the prothrombin defect and a number of red cell and plasma protein genetic markers. 5 The nature of the structural defect in "prothrombin Cardeza" was studied by biologic and immunologic methods. Deficient thrombin elaboration when patient plasma is activated in a normal plasma system (intrinsic activation, Fig.…”

Radioimmunoassay of human prothrombin—The quantitation of plasma factor II antigen

“…Josso and colleagues in 1967 described a semiquantitative measurement of factor I1 by employing an immunologic method using precipitating antibodies to human prothrombin [9] . The following year, Ganrot and Nilehn published an imniunoelectrophoretic method to quantitate the prothrombin antigen in plasma using guinea pig antihuman factor 11 [lo].…”