Etv5, an ETS transcription factor, is expressed in granulosa and cumulus cells and serves as a transcriptional regulator of the cyclooxygenase-2

Eo, Jinwon; Han, Katrina; Murphy, Kenneth M.; Song, Haengseok; Lim, Hyunjung Jade

doi:10.1677/joe-08-0142

Cited by 28 publications

(31 citation statements)

References 35 publications

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“…The cooperation between ETV5 and LPP at the nuclear level was confirmed by luciferase experiments using a luciferase reporter vector carrying the promoter region of PTGS2, as a known target of ETV5 transcription factor 28 (see Supplementary Figure 4). Hec1A cells overexpressing LPP showed increased ETV5 dependent transcriptional activity compared with cells expressing basal LPP levels ( Figure 5d).…”

Section: Etv5 Promotes Emt In Hec1a Cellsmentioning

confidence: 82%

ETV5 cooperates with LPP as a sensor of extracellular signals and promotes EMT in endometrial carcinomas

et al. 2012

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Endometrial carcinoma (EC) is the most frequent among infiltrating tumors of the female genital tract, with myometrial invasion representing an increase in the rate of recurrences and a decrease in survival. We have previously described ETV5 transcription factor associated with myometrial infiltration in human ECs. In this work, we further investigated ETV5 orchestrating downstream effects to confer the tumor the invasive capabilities needed to disseminate in the early stages of EC dissemination. Molecular profiling evidenced ETV5 having a direct role on epithelial-to-mesenchymal transition (EMT). In particular, ETV5 modulated Zeb1 expression and E-Cadherin repression leading to a complete reorganization of cell --cell and cell --substrate contacts. ETV5-promoted EMT resulted in the acquisition of migratory and invasive capabilities in endometrial cell lines. Furthermore, we identified the lipoma-preferred partner protein as a regulatory partner of ETV5, acting as a sensor for extracellular signals promoting tumor invasion. All together, we propose ETV5-transcriptional regulation of the EMT process through a crosstalk with the tumor surrounding microenvironment, as a principal event initiating EC invasion.

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Section: Etv5 Promotes Emt In Hec1a Cellsmentioning

confidence: 82%

ETV5 cooperates with LPP as a sensor of extracellular signals and promotes EMT in endometrial carcinomas

et al. 2012

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“…39 Furthermore, ERM has been reported to function as a transcriptional activator. 40 It also is known to have regulatory functions in many types of cancers, such as prostate 41 and ovarian cancers. 42 ERM also enhances intestinal epithelial tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

Altered Expression of the Poly(ADP-Ribosyl)ation Enzymes in Uveal Melanoma and Regulation ofPARGGene Expression by the Transcription Factor ERM

Molloy-Simard

St-Laurent

Vigneault

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Poly(ADP-ribosyl)ation is a reversible post-translational modification that requires the contribution of the enzymes poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG). Our study explores expression and activity of PARP-1 and PARG in uveal melanoma cell lines with varying tumorigenic properties.METHODS. Gene profiling on microarrays was conducted using RNA prepared from the uveal melanoma cell lines T97, T98, T108, and T115. The activity of PARP-1 and PARG was monitored by enzymatic assays, whereas their expression was measured by Western blot and PCR. The PARG promoter was analyzed using promoter deletions and site-specific mutagenesis in transfection analyses. The transcription factors binding the PARG promoter were studied by electrophoretic mobility shift assay (EMSA) analyses. Suppression of PARP-1 and PARG expression was performed in T97 and T115 cells by RNAi, and their tumorigenic properties monitored by injections into athymic mice.RESULTS. Expression of PARP-1 was found to vary considerably between uveal melanoma cell lines with distinctive tumorigenic properties in vivo. Sp1 and the ETS protein ERM were shown to bind to the PARG gene promoter to ensure basal transcription in uveal melanoma. Importantly, suppression of PARG gene expression in T97 and T115 cells increased their capacity to form tumors in athymic mice, whereas suppression of PARP-1 significantly reduced or almost entirely abolished tumor formation. CONCLUSIONS.Our results suggest that while overexpression of PARP-1 may confer a proliferative advantage to aggressive uveal melanoma tumors, PARG may, on the other hand, support a tumor suppressor function in vivo. (Invest Ophthalmol Vis Sci.

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“…More recently, the expression of members of the ETV4 subfamily was found to be reduced in the limb bud and brain of FGF and/or FGFR knock-out mice embryos (Cholfin and Rubenstein, 2008;Zhang et al, 2009). As ETV4 (also known as PEA3) and ETV5 (also known as ERM) mRNA and protein were identified in murine granulosa and cumulus cells (Eo et al, 2008), we assessed whether these are regulated by FGF2. Messenger (10 ng/ml) transiently increased abundance of mRNA encoding NR4A1 but not NR4A2 or NR4A3.…”

Section: Discussionmentioning

confidence: 99%

Fibroblast growth factor-2 regulation of sprouty and NR4A genes in bovine ovarian granulosa cells

et al. 2011

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Fibroblast growth factors (FGFs) alter ovarian function, at least in part by inhibiting steroid hormone secretion and affecting survival of granulosa cells. The mechanism of action of FGFs in ovarian follicle cells is largely unknown; in the present study we identified the major pathways used by FGF2 in non-luteinizing granulosa cells cultured under serum-free conditions. FGF2 increased abundance of mRNA encoding SPRY1, 2, and 4, but not SPRY3. Common pathways employed by FGF2 in the regulation of SPRY1, 2, and 4, as demonstrated by immunoblot and inhibitor studies, included ERK1/2 and Akt signaling. In contrast, PKC activation was necessary for FGF2-stimulated expression of SPRY1 and 4, but not for SPRY2. Intracellular calcium flux is critical and sufficient for SPRY2 expression, but not for SPRY1 and 4. We also identified the orphan nuclear receptor NR4A1 as a potential early response gene in FGF2 signaling, whose expression, like that of SPRY2, is critically dependent on calcium signaling. Together, these data identify FGF2-target genes in follicular granulosa cells, and demonstrate alternative pathway use for the differential control of SPRY genes.

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Etv5, an ETS transcription factor, is expressed in granulosa and cumulus cells and serves as a transcriptional regulator of the cyclooxygenase-2

Cited by 28 publications

References 35 publications

ETV5 cooperates with LPP as a sensor of extracellular signals and promotes EMT in endometrial carcinomas

ETV5 cooperates with LPP as a sensor of extracellular signals and promotes EMT in endometrial carcinomas

Altered Expression of the Poly(ADP-Ribosyl)ation Enzymes in Uveal Melanoma and Regulation ofPARGGene Expression by the Transcription Factor ERM

Fibroblast growth factor-2 regulation of sprouty and NR4A genes in bovine ovarian granulosa cells

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