Eu(III)–DO3A and BODIPY dyad as a chemosensor for anthrax biomarker

Abstract

The sensitive and selective determination of Bacillus anthracis spores before the infection is vital for human health and safety. Dipicolinic acid (DPA) is an excellent biomarker due to its presence in the nucleus of bacterial spores at high concentrations (up to 1 M, about 15% dry weight). In the present work, a new molecular chemosensor 1, based on europium(III)–DO3A and BODIPY dyad, is developed to detect DPA in phosphate‐buffered saline (PBS) buffered solution and tap water samples. Also, 1 can be used as a ratiometric optical chemosensor to track DPA.

A terbium-functionalized ZIF-8 nanosensor for rapid and sensitive detection of an anthrax spore biomarker

“…6,7 Dipicolinic acid (DPA) has been used as a biomarker (the major constituent of bacterial spores) for anthrax spore detection. 8,9 Numerous detection methods, including biological methods, electrochemical detection, polymerase chain reaction (PCR), and surface-enhanced Raman spectroscopy (SERS), have been developed for detecting DPA. 10,11 However, the above methods have some defects, such as lengthy cycles, complicated pretreatment, expensive reagents, long testing time, or professional analysis, making them unsuitable for rapid and real-time monitoring.…”

Facilitated endospore detection for Bacillus spp. through automated algorithm‐based image processing

“…As mentioned before, DPA acts as a suitable biomarker and is often used for the spore detection as well [ 9 ]. DPA can either be detected by a historic colorimetric assay from the 1950‐1960s, using ammonium iron(II) sulfate [ 16 , 17 ] or in more recent publications with lanthanides such as europium(III) or terbium(III) [ 18 , 19 , 20 , 21 , 22 ]. Those assays have no special technical requirements and lead to a significant time‐saving compared to CFU.…”