Compost produced from biological treatment of organic waste has a potential for substituting peat in growth media preparation. The Life Cycle Inventories (LCIs) of the two alternatives were compared using LCA-modelling (EASEWASTE) considering a 100 year period and a volumetric substitution ratio of 1:1. For the compost alternative, the composting process, growth media use, and offsetting of mineral fertilizers were considered. For the peat alternative, peat-land preparation, excavation, transportation, and growth media use were considered. It was assumed that for compost 14% of the initial carbon was left in the soil after 100 years, while all carbon in peat was mineralized. With respect to greenhouse gas emissions, the former is considered a saving, while the later is considered an emission, because peat in a peatland is considered stored biogenic carbon. The leaching during the growth media use was assessed by means of batch leaching tests involving 4 compost samples and 7 peat samples. The compost leached 3-20 times more heavy metals and other compounds than the peat. The life-cycle-assessment showed that compost performs better regarding global warming (savings in the range of 70-150 kg CO 2 -eq. Mg -1 ) and nutrient enrichment (savings in the range of 1.7-6.8 kg NO 3 Mg -1 compost), while peat performs better in some toxic categories, because of the lower content of heavy metals.