Eukaryotic Adaptation to Years-Long Starvation Resembles that of Bacteria

Aouizerat, Tzemach; Gelman, Daniel; Szitenberg, Amir; Gutman, Itay; Glazer, Shunit; Reich, Eli; Schoemann, Miriam; Kaplan, Rachel; Saragovi, Amijai; Hazan, Ronen; Klutstein, Michael

doi:10.1016/j.isci.2019.08.002

Cited by 15 publications

(18 citation statements)

References 42 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In E. coli, the stationary phase period is followed by a type of programed cell death and selection of survivors exhibiting the GASP phenotype 50,51 that results mainly from mutations reducing the activity of the sigma S/ sigma38 transcription factor (RpoS), the general starvation/stressresponse transcriptional regulator 52,53 . Thus, similarly to the relationship previously proposed for the closely linked mTOR pathway 42,54 , the fission yeast S/MAPK pathway plays a function analogous to RpoS in bacteria. This similarity indicates that the stress-response pathways are central to the genetics of quiescence.…”

Section: Discussionsupporting

confidence: 66%

“…In budding yeast, high-throughput screening identified HOG1, SSK2 (SAPK pathway), and SGF73 in glucoseand sulfate-limited conditions, respectively 32,41 . Recently, HOG1 was also found mutated in yeast after a 2-year starvation in sealed 500 750 1000 0 250 500 750 1000 0 250 500 750 1000 0 250 500 750 1000 0 250 500 750 1000 0 250 500 750 1000 0 250 500 750 1000 0 250 500 750 1000 0 250 500 bottles of beer 42 . Thus, mutating the stress-response pathway emerges as a common strategy to promote a growth advantage under different starvation conditions in two unrelated yeast, indicating a high degree of converging evolution 43 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways

et al. 2020

View full text Add to dashboard Cite

The genetics of quiescence is an emerging field compared to that of growth, yet both states generate spontaneous mutations and genetic diversity fueling evolution. Reconciling mutation rates in dividing conditions and mutation accumulation as a function of time in non-dividing situations remains a challenge. Nitrogen-starved fission yeast cells reversibly arrest proliferation, are metabolically active and highly resistant to a variety of stresses. Here, we show that mutations in stress-and mitogen-activated protein kinase (S/MAPK) signaling pathways are enriched in aging cultures. Targeted resequencing and competition experiments indicate that these mutants arise in the first month of quiescence and expand clonally during the second month at the expense of the parental population. Reconstitution experiments show that S/MAPK modules mediate the sacrifice of many cells for the benefit of some mutants. These findings suggest that non-dividing conditions promote genetic diversity to generate a social cellular environment prone to kin selection.

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Looking for evidence of adaptive evolution through convergence of mutations, we found that between experiments, there were 5 genes wherein multiple mutations were observed in the coding sequence between experiments (Table 2). We note that mutations in UBP1, which encodes a ubiquitin protease, were previously identified in experimental evolution of a lager strain [34], and mutations in TFB1, a nucleotide excision repair factor and subunit of TFIIH, were found in strains that had survived for two years in a sealed beer bottle [35]. However, in neither of these cases were phenotypic consequence proven.…”

Section: Resultsmentioning

confidence: 88%

Genomic stability and adaptation of beer brewing yeasts during serial repitching in the brewery

Large

Hanson

Tsouris

et al. 2020

Preprint

View full text Add to dashboard Cite

AbstractAle brewing yeast are the result of admixture between diverse strains of Saccharomyces cerevisiae, resulting in a heterozygous tetraploid that has since undergone numerous genomic rearrangements. As a result, comparisons between the genomes of modern related ale brewing strains show both extensive aneuploidy and mitotic recombination that has resulted in a loss of intragenomic diversity. Similar patterns of intraspecific admixture and subsequent selection for one haplotype have been seen in many domesticated crops, potentially reflecting a general pattern of domestication syndrome between these systems. We set out to explore the evolution of the ale brewing yeast, to understand both polyploid evolution and the process of domestication in the ecologically relevant environment of the brewery. Utilizing a common brewery practice known as ‘repitching’, in which yeasts are reused over multiple beer fermentations, we generated population time courses from multiple breweries utilizing similar strains of ale yeast. Applying whole-genome sequencing to the time courses, we have found that the same structural variations in the form of aneuploidy and mitotic recombination of particular chromosomes reproducibly rise to detectable frequency during adaptation to brewing conditions across multiple related strains in different breweries. Our results demonstrate that domestication of ale strains is an ongoing process and will likely continue to occur as modern brewing practices develop.

show abstract

“…We have investigated the adaptation process that S. cerevisiae cells undergo after two years of starvation in a stressful environment of a beer bottle (Aouizerat et al, 2019a). We found striking lines of similarity between characteristics of GASP in bacteria and yeast.…”

Section: Epnp Phase In Eukaryotic Cellsmentioning

confidence: 99%

Endurance of extremely prolonged nutrient prevention across kingdoms of life

2021

Self Cite

View full text Add to dashboard Cite

Numerous observations demonstrate that microorganisms can survive very long periods of nutrient deprivation and starvation. Moreover, it is evident that prolonged periods of starvation are a feature of many habitats, and many cells in all kingdoms of life are found in prolonged starvation conditions. Bacteria exhibit a range of responses to long-term starvation. These include genetic adaptations such as the long-term stationary phase and the growth advantage in stationary phase phenotypes characterized by mutations in stress-signaling genes and elevated mutation rates. Here, we suggest using the term "endurance of prolonged nutrient prevention" (EPNP phase), to describe this phase, which was also recently described in eukaryotes. Here, we review this literature and describe the current knowledge about the adaptations to very long-term starvation conditions in bacteria and eukaryotes, its conceptual and structural conservation across all kingdoms of life, and point out possible directions that merit further research.

show abstract

Eukaryotic Adaptation to Years-Long Starvation Resembles that of Bacteria

Cited by 15 publications

References 42 publications

Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways

Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways

Genomic stability and adaptation of beer brewing yeasts during serial repitching in the brewery

Endurance of extremely prolonged nutrient prevention across kingdoms of life

Contact Info

Product

Resources

About