EuNPs-mAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of porcine epidemic diarrhea virus

Xu, Fei; Jin, Zhongwu; Zou, Siyi; Chen, Chaoqun; Song, Qibin; Deng, Shulin; Xiao, Wei; Zhang, Xiaoli; Jia, Aiqing; Tang, Yong

doi:10.1016/j.talanta.2020.120865

Cited by 21 publications

(17 citation statements)

References 30 publications

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“…In this study, we integrated membrane filtration and an LFIA platform to achieve sample pretreatment without additional operation. A filtration unit was applied to improve the analytical performance of LBs-LFIA for the analysis of swine feces and was found to yield good agreement (92.59%) with RT-PCR results, which was much higher than that of the reported colloidal gold LFAs (74.07%) (Bian et al 2019) and fluorescent LFAs (86.67%) (Xu et al 2020). These results indicate that the LBs-LFIA is sensitive, specific and allowed on-site user-operated detection of PEDV, which could shorten the response time for dealing with potential disease outbreaks.…”

Section: Introductionmentioning

confidence: 93%

Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Zou

et al. 2021

Animal Diseases

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Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry. Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease. In this study, a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label. Under optimal conditions, the newly developed latex bead-based lateral flow immunoassay (LBs-LFIA) attained a limit of detection (LOD) as low as 103.60 TCID50/mL and no cross-reactivity with other related swine viruses. To solve swine feces impurity interference, by adding a filtration unit design of LFIA without an additional pretreatment procedure, the LBs-LFIA gave good agreement (92.59%) with RT-PCR results in the analysis of clinical swine fecal samples (n = 108), which was more accurate than previously reported colloidal gold LFIA (74.07%) and fluorescent LFIA (86.67%). Moreover, LBs-LFIA showed sufficient accuracy (coefficient of variance [CV] < 15%) and stable (room temperature storage life > 56 days) performance for PEDV detection, which is promising for on-site analysis and user-driven testing in pig production system.

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Section: Introductionmentioning

confidence: 93%

Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Zou

et al. 2021

Animal Diseases

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“…PVPK-30 has good dispersibility and can eliminate the background color of NC film. According to the literature [ 23 ], sucrose, BSA, Tween-20, and PVPK-30 were successively added to ultra-pure water with volume ratios of 1%, 0.5%, 2%, and 1% to obtain the reaction sustained release solution. Time-resolved fluorescence detection was performed by immunochromatographic strips and time-resolved fluorescence speedometer.…”

Section: Methodsmentioning

confidence: 99%

Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol

Dong

Xiang

et al. 2020

Sensors

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Immunoassay has the advantages of high sensitivity, high specificity, and simple operation, and has been widely used in the detection of mycotoxins. For several years, time-resolved fluorescence immunochromatography (TRFIA) paper-based sensors have attracted much attention as a simple and low-cost field detection technology. However, a traditional TRFIA paper-based sensor is based on antibody labeling, which cannot easily meet the current detection requirements. A second antibody labeling method was used to amplify the fluorescence signal and improve the detection sensitivity. Polystyrene fluorescent microspheres were combined with sheep anti-mouse IgG to prepare fluorescent probes (Eu-IgGs). After the probe fully reacted with the antibody (Eu-IgGs-Abs) in the sample cell, it was deployed on the paper-based sensor using chromatography. Eu-IgGs-Abs that were not bound to the target were captured on the T-line, while those that were bound were captured on the C-line. The paper-based sensor reflected the corresponding fluorescence intensity change. Because a single molecule of the deoxynivalenol antibody could bind to multiple Eu-IgGs, this method could amplify the fluorescence signal intensity on the unit antibody and improve the detection sensitivity. The working standard curve of the sensor was established under the optimum working conditions. It showed the lower limit of detection and higher recovery rate when it was applied to actual samples and compared with other methods. This sensor has the advantages of high sensitivity, good accuracy, and good specificity, saving the amount of antibody consumed and being suitable for rapid field detection of deoxynivalenol.

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“…In previous studies, applications based on nanozyme catalyzed color development have been reported. [25] , [26] The nanozymes, such as Au@PtNPs, are nanomaterial with intrinsic enzyme mimicking activity, which have good catalytic activity and stability, presenting low cost and easy coupling, thus widely used in the various biosensors. [27] , [28] , [29] Compared with gold nanoparticles (AuNPs), the gold-platinum nanoparticles (Au@PtNPs) realized the signal amplification on color reaction, consequently the sensitivity was greatly improved.…”

Section: Introductionmentioning

confidence: 99%

A nanoenzyme linked immunochromatographic sensor for rapid and quantitative detection of SARS-CoV-2 nucleocapsid protein in human blood

Liang

Liu

et al. 2021

Sensors and Actuators B: Chemical

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The establishment of a simple, low-cost, high-sensitive and rapid immunoassay for detecting SARS-CoV-2 antigen in human blood is an effective mean of discovering early SARS-CoV-2 infection and controlling the pandemic of COVID-19. Herein, a smartphone based nanozyme linked immunochromatographic sensor (NLICS) for the detection of SARS-CoV-2 nucleocapsid protein (NP) has been developed on demand. The system is integrated by disposable immunochromatography assay (ICA) and optical sensor devices. Immunoreaction and enzyme-catalyzed substrate color reaction were carried out on the chromatographic strip in a device, of which the light signal was read by a photometer through a biosensor channel, and the data was synchronously transmitted via the Bluetooth to the app in-stored smartphone for reporting the result. With a limit of detection (LOD) of 0.026 ng/mL NP, NLICS had the linear detection range (LDR) between 0.05 and 1.6 ng/mL NP, which was more sensitive than conventional ICA. NLICS took 25 min for reporting results. For detection of NP antigen in clinical serum samples from 21 COVID-19 patients and 80 healthy blood donor controls, NLICS and commercial enzyme linked immunosorbent assay (ELISA) had 76.2% or 47.6% positivity, and 100% specificity, respectively ( P =0.057), while a good correlation coefficient (r=0.99) for quantification of NP between two assays was obtained. In conclusion, the NLICS was a rapid, simple, cheap, sensitive and specific immunochromatographic sensing assay for early diagnosis of SARS-CoV-2 infection.

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EuNPs-mAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of porcine epidemic diarrhea virus

Cited by 21 publications

References 30 publications

Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol

A nanoenzyme linked immunochromatographic sensor for rapid and quantitative detection of SARS-CoV-2 nucleocapsid protein in human blood

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