Eupatilin inhibits H2O2-induced apoptotic cell death through inhibition of mitogen-activated protein kinases and nuclear factor-κB

Lee, So-Young; Lee, Myeungsu; Kim, Sang-Hyun

doi:10.1016/j.fct.2008.05.026

Cited by 47 publications

(35 citation statements)

References 25 publications

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“…Recently Choi et al (2009) has reported the novel antitumor, cytotoxic activity of Eupatilin through robust induction of apoptosis in human gastric carcinoma AGS cells complementing the previous findings by Kim at al. (2005b) and Lee et al (2008). Similar inhibitory effects were also documented by Kim al.…”

Section: Introductionsupporting

confidence: 69%

“…The effects of the Eupatilin on other malignant cell lines were reported previously. One study was done by Lee et al (2008) and the other by Kim (2005) and associates reported the cytotoxicity of Eupatilin on gastric cancer (Kim et al, 2005b;Lee et al, 2008). Other studies also reported cytotoxic activity of Eupatilin against breast cancer (Kim et al, 2005a), leukemia (Seo and Surh, 2001), mast tumor (Koshihara et al, 1983) and colon cancer cells (Nam et al, 2008;Schulze-Osthoff et al, 1994;Wyllie, 1980;Zhang and Xu, 2000).…”

Section: Cell Growth Inhibition Of A375 Melanoma Cells By Eupatilinmentioning

confidence: 95%

“…There are many chemopreventive agents, which result in cancer cell cell death by induction of apoptosis (Kelloff et al, 2000). Previous studies have shown that Eupatilin induces potent growth inhibition in many tumor cell lines (Kim et al, 2005b;Lee et al, 2008). It was reported that Eupatilin induced apoptosis of MCF10A-ras breast cancer cells.…”

Section: Induction Of Apoptosis In A375 Melanoma Cells By Eupatilinmentioning

confidence: 99%

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Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Shawi#¹

2011

Afr. J. Pharm. Pharmacol.

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Eupatilin is a flavonoid compound isolated from the Artemisia plant. Eupitillin possesses antioxidant as well as potent anticancer properties. In this study, for the first time, we examined the anti-proliferative effects of Eupatilin in human melanoma A375 cells and its ability to induce apoptosis and cell cycle arrest. Eupatilin specifically induced morphological changes in A375 cells and was less toxic to the normal mouse splenocytes. The inhibitory effects of A375 cells were associated with the DNA damage, apoptosis, and cell cycle arrest at G2/M phase in a dose-dependent manner. The apoptotic effects of Eupatilin were further verified by using Annexin V-FITC/propidium iodide staining in flow cytometry. These results suggest that Eupatilin is an effective natural compound that worth further mechanistic and therapeutic studies against human melanoma.

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Section: Introductionsupporting

confidence: 69%

Section: Cell Growth Inhibition Of A375 Melanoma Cells By Eupatilinmentioning

confidence: 95%

Section: Induction Of Apoptosis In A375 Melanoma Cells By Eupatilinmentioning

confidence: 99%

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Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Shawi#¹

2011

Afr. J. Pharm. Pharmacol.

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“…The tablet was added to the dissolution medium (900 mL of acidic buffer solution at pH 1.2 supplemented with 0.5 % SLS) without a sinker and rotated at a speed of 50 rpm at 37.0 ± 0.2 °C (n =12). Samples were collected at specific time intervals (5,10,15,30,45, and 60 min) to analyze the amount of eupatilin using HPLC.…”

Section: Dissolution Rate Of Tablets At Ph 12 (Determination Of Disimentioning

confidence: 99%

“…It is well known that Artemisia possesses many biological active phytochemicals such as flavonoids, alkaloids, phenols, steroids, and essential oils. Eupatilin (5,7-dihydroxy-3´,4´,6 trimethoxy flavone) is the main active compound isolated from Artemisia plants and has a variety of biological activities, such as anti-inflammatory, anti-oxidative, cytoprotective, and pro-or anti-apoptotic effects (9,10). Many Artemisia species have biological activities, such as anti-inflammatory, anti-bacterial, anti-asthmatic, anti-cancerous, and neuroprotective activities.…”

mentioning

confidence: 99%

Gastroprotective effects of the isopropanol extract of Artemisia princeps and its gastroretentive floating tablets on gastric mucosal injury

et al. 2017

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In this study, we investigated the gastroprotective effect of an isopropanol extract from the aerial parts of Artemisia princeps (IPAP) and developed a gastroretentive floating tablet of IPAP (IPAP-FR) for maximized local gastroprotective effects. Pre-treatment with IPAP ameliorated the gastric mucosal hemorrhagic lesions in ethanol/HCl-or indomethacin-treated rats. IPAP decreased mucosal hemorrhage of gastric ulcers induced by ethanol or indomethacin plus pyloric ligation in rats. The optimized floating tablet, IPAP-FR, floated on medium surface with more sustained eupatilin release compared to the non-floating control tablet. X-ray photographs in beagle dogs showed that IPAP-FR was retained for > 2 h in the stomach. In the ethanol-induced gastric ulcer rat model, the gastric hemorrhagic lesion was improved more substantially with IPAP-FR compared to the non-floating control tablet. Based on these data, our data suggest that IPAP-FR has an improved therapeutic potential for the treatment of gastric ulcer.

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Eupatilin inhibits T‐cell activation by modulation of intracellular calcium flux and NF‐κB and NF‐AT activity

Kim¹,

Choi

et al. 2009

J of Cellular Biochemistry

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Eupatilin, one of the pharmacologically active ingredients of Artemisia princeps, exhibits a potent anti-ulcer activity, but its effects on T-cell immunity have not been investigated. Here, we show that eupatilin has a profound inhibitory effect on IL-2 production in Jurkat T cells as well as in human peripheral blood leukocytes. Eupatilin neither influenced clustering of CD3 and LFA-1 to the immunological synapse nor inhibited conjugate formation between T cells and B cells in the presence or absence of superantigen (SEE). Eupatilin also failed to inhibit T-cell receptor (TCR) internalization, thereby, suggesting that eupatilin does not interfere with TCR-mediated signals on the membrane proximal region. In unstimulated T cells, eupatilin significantly induced apoptotic cell death, as evidenced by an increased population of annexin V(+)/PI(+) cells and cleavage of caspase-3 and PARP. To our surprise, however, once cells were activated, eupatilin had little effect on apoptosis, and instead slightly protected cells from activation-induced cell death, suggesting that apoptosis also is not a mechanism for eupatilin-induced T-cell suppression. On the contrary, eupatilin dramatically inhibited I-kappaBalpha degradation and NF-AT dephosphorylation and, consequently, inhibited NF-kappaB and NF-AT promoter activities in PMA/A23187-stimulated T cells. Interestingly, intracellular calcium flux was significantly perturbed in cells pre-treated with eupatilin, suggesting that calcium-dependent cascades might be targets for eupatilin action. Collectively, our results provide evidence for dual regulatory functions of eupatilin: (1) a pro-apoptotic effect on resting T cells and (2) an immunosuppressive effect on activated T cells, presumably through modulation of Ca(2+) flux.

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Eupatilin inhibits H2O2-induced apoptotic cell death through inhibition of mitogen-activated protein kinases and nuclear factor-κB

Cited by 47 publications

References 25 publications

Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Gastroprotective effects of the isopropanol extract of Artemisia princeps and its gastroretentive floating tablets on gastric mucosal injury

Eupatilin inhibits T‐cell activation by modulation of intracellular calcium flux and NF‐κB and NF‐AT activity

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