Euploidy rates in donor egg cycles significantly differ between fertility centers

Munné, Santiago; Alikani, Mina; Ribustello, L.; Colls, P.; Martínez-Ortiz, Pedro A.; McCulloh, David H.

doi:10.1093/humrep/dex031

Cited by 116 publications

(63 citation statements)

References 54 publications

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“…Moreover, other studies and reports, in which the patient cohort was made up of only oocyte donors, the pregnancy rate was around 50% [28,29]. Also, the aneuploidy rate could be around 25%, and, depending on the age of the sperm donor, exceed 60% [9, 10, 30]. Therefore, we believe that the oocyte donor group does represent the typical donor make‐up of Central Mexicans.…”

Section: Discussionmentioning

confidence: 84%

“…These hormones are shown to affect protein synthesis, cellular division, and membrane fluidity and structure—key factors associated with embryo implantation [8]. Moreover, the aneuploidy rate of donor oocyte derived embryos, which ranges between 20 and 60% [9], are similar to patient oocyte derived embryos, when the patient's age is above 27 years [10]. Interestingly, Munne and colleagues demonstrated that the euploidy rate is associated with IVF clinics, thus indicating the procedural, material, and professional experience and preferences lead to a clinic effect [9].…”

Section: Introductionmentioning

confidence: 99%

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Embryos derived from donor or patient oocytes are not different for in vitro fertilization outcomes when PGT allows euploid embryo selection: a retrospective study

Schaeffer

Porchia

Neumann³

et al. 2020

Clinical & Translational Med

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BackgroundAt our facilities, patients that received embryos using donor oocyte during in vitro fertilization (IVF), usually have had at least one failed attempt to produce at least one euploid embryo with their own oocytes; however, the current debate between using donor over patient oocytes remains inconclusive. We examined the aneuploidy rate and IVF clinical outcomes from embryos derived from either donor or patient oocytes. MethodsRetrospectively, 973 cycles were examined of patients who underwent a standard IVF protocol. Chromosomal content was determined using Pre‐implantation Genetic Testing (PGT) by either microarray‐comparative genomic hybridization or Next‐generation sequencing from either Day 3 (blastocysts) or Day 5 (trophectoderm) embryo biopsies, respectively. Embryo implantation was confirmed by serum β‐hCG (> 10 m IU/mL/Day 14), whereas clinical pregnancy by a fetal heartbeat (Week 6.5–8). ResultsEmbryos derived from donor oocytes presented with more monosomies than embryos derived from patient oocytes (41.2% vs. 25.4%, p < 0.05, respectively); however, only Trisomy 7 (0.4% vs. 2.3%, p < 0.05) and Trisomy in X (0.7% vs. 2.3%, p < 0.05) were significantly less present when compared to patient oocyte derived embryos. Interestingly, rates for embryo implantation (46.7% vs. 50.8%, p = 0.35), clinical pregnancy (38.5% vs. 43.1%, p = 0.30), and live birth (30.5% vs. 30.5%, p = 0.99) were similar for embryos derived from donor and patient oocytes. These results did not change when adjusted for the number of embryos implanted. ConclusionHere, we show no significant differences in achieving pregnancy when using donor oocytes. Taking into consideration that aneuploidy rates are > 30% in embryos, independent of the oocyte origin, PGT should be recommended with donor oocytes as well.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Embryos derived from donor or patient oocytes are not different for in vitro fertilization outcomes when PGT allows euploid embryo selection: a retrospective study

Schaeffer

Porchia

Neumann³

et al. 2020

Clinical & Translational Med

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“…In mouse models, changes in oxygen tension during embryo culture have been shown to affect chromosomal mosaicism rates . Recently, the incidence of chromosome abnormalities in human embryos has been shown to be fertility center‐dependent, indicating that mosaicism rate could very probably be influenced by culture conditions as temperature, pH and media composition …”

Section: Weaknessesmentioning

confidence: 99%

“…29 Recently, the incidence of chromosome abnormalities in human embryos has been shown to be fertility center-dependent, indicating that mosaicism rate could very probably be influenced by culture conditions as temperature, pH and media composition. 30 Although embryonic mosaicism was initially observed 25 years ago, 31 the emerging attribution of embryos as mosaics are because of two phenomena. First, genetic technologies for detecting chromosomal copy number variations in embryos have evolved from FISH to CCS platforms that represent a superior method for the assessment of mosaicisms.…”

Section: Management Of Mosaicismsmentioning

confidence: 99%

Assessment of pre‐implantation genetic testing for embryo aneuploidies: A SWOT analysis

et al. 2019

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The recently re‐named pre‐implantation genetic testing for determining embryo aneuploidies (PGT‐A) is presently very popular although its acceptance by the scientific community is controversial. This approach still encounters drawbacks. This paper uses a SWOT (strengths, weaknesses, opportunities and threats) analysis to discuss salient points to be considered when examining the pre‐implantation genetic testing (PGT‐A) strategy to gather information from a range of perspectives. One of the strengths associated with the procedure is represented by an increase in implantation rate although data from the highest level of evidence do not support an increase in cumulative pregnancy rates. The current difficulty in the management of mosaicisms represents a weakness of PGT‐A. The application of the strategy represents an opportunity to favor the single embryo transfer while other advantages, such as reduction of time to pregnancy and emotional distress are controversial. Potential important threats, at present still undefined, are represented by the biopsy‐related damage to the blastocyst and the impact on neonatal and long‐term outcomes.

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“…Several studies have used this model obtaining high pregnancy rates and good obstetrical outcomes in recipients ( Sauer & Kavic, 2006 ; Budak et al ., 2007 ). Regarding chromosome abnormalities in embryos of egg donors, several studies reveal percentages of aneuploidy embryos to be between 39-82% ( Reis Soares et al ., 2003 ; Munné et al ., 2006 ; Sills et al ., 2014 ; García-Ferreyra et al ., 2015 ; Haddad et al ., 2015 ; Munné et al. , 2017 ), and only García-Ferreyra et al .…”

Section: Introductionmentioning

confidence: 97%

High percentages of embryos with 21, 18 or 13 trisomy are related to advanced paternal age in donor egg cycles

García-Ferreyra¹,

Hilario

Dueñas³

2018

JBRA Assisted Reproduction

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ObjectiveAdvanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes.MethodsA total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos). Fertilization rates, embryo quality at day 3, blastocysts development, and aneuploidy embryo rates were then compared.ResultsThere was no difference in seminal parameters (volume, concentration and motility) in the studied groups. Fertilization rate, percentages of zygotes that underwent cleavage, and good-quality embryos on Day 3 were similar between the three groups evaluated. The group of men ≥50 years had significantly more sperm with damaged DNA, higher global aneuploidy rates, and significantly more embryos with trisomy 21, 18 or 13 compared to the other two evaluated groups (p<0.05).ConclusionOur data shows that advanced paternal age increases global chromosomal abnormalities, and percentages of trisomy 21, 18 or 13 in embryos, and such effect is significantly important as of the age of 50. Embryo genetic screening is highly recommended in patients in which paternal age is ≥50 years old.

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Euploidy rates in donor egg cycles significantly differ between fertility centers

Abstract: No external funds were used for this study. S. Munne is a founding principle of Reprogenetics/current employee of Cooper Genomics. M Alikani's spouse is a founding principle of Reprogenetics/current consultant for Cooper Genomics. The remaining authors have no conflicts to declare.

Cited by 116 publications

References 54 publications

Embryos derived from donor or patient oocytes are not different for in vitro fertilization outcomes when PGT allows euploid embryo selection: a retrospective study

Embryos derived from donor or patient oocytes are not different for in vitro fertilization outcomes when PGT allows euploid embryo selection: a retrospective study

Assessment of pre‐implantation genetic testing for embryo aneuploidies: A SWOT analysis

High percentages of embryos with 21, 18 or 13 trisomy are related to advanced paternal age in donor egg cycles

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