European ferns as rich sources of antioxidants in the human diet

Langhansová, Lenka; Pumprova, Karolína; Haisel, D.; Ekrt, Libor; Pavičić, Antonio; Zajíčková, Markéta; Vaněk, Tomáš; Dvořáková, Marcela

doi:10.1016/j.foodchem.2021.129637

Cited by 24 publications

(14 citation statements)

References 61 publications

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“…For isolation of the hepatocytes, a two-step collagenase method was used, i.e. a piece of liver was firstly perfused by EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid) containing solution (0.14 mg/mL), then perfused by collagenase solution (1 mg/mL) [ 17 ]. The viability of the hepatocytes was tested using a Trypan Blue exclusion assay (Trypan Blue solution 0.4%).…”

Section: Methodsmentioning

confidence: 99%

Sertraline as a new potential anthelmintic against Haemonchus contortus: toxicity, efficacy, and biotransformation

Zajíčková

Prchal

Navrátilová

et al. 2021

Vet Res

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Haemonchus contortus is a parasitic nematode of ruminants which causes significant losses to many farmers worldwide. Since the drugs currently in use for the treatment of haemonchosis are losing their effectiveness due to the drug-resistance of this nematode, a new or repurposed drug is highly needed. As the antipsychotic drug sertraline (SRT) has been shown to be effective against the parasitic nematodes Trichuris muris, Ancylostoma caninum and Schistosoma mansoni, the aim of the present study was to evaluate the possible effect of SRT on H. contortus. The potential hepatotoxicity of SRT was tested in sheep, a common H. contortus host. In addition, the main metabolic pathways of SRT in H. contortus and the ovine liver were identified. While no effect of SRT on H. contortus egg hatching was observed, SRT was found to significantly decrease the viability of H. contortus adults in drug-sensitive and resistant strains, with its effect comparable to the commonly used anthelmintics levamisole and monepantel. Moreover, SRT in anthelmintically active concentrations showed no toxicity to the ovine liver. Biotransformation of SRT in H. contortus was weak, with most of the drug remaining unmetabolized. Production of the main metabolite hydroxy-SRT did not differ significantly between strains. Other minor metabolites such as SRT-O-glucoside, dihydroxy-SRT, and SRT-ketone were also identified in H. contorts adults. Compared to H. contortus, the ovine liver metabolized SRT more extensively, mainly via desmethylation and glucuronidation. In conclusion, the potency of SRT against H. contortus was proven, and it should be tested further toward possible repurposing.

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Section: Methodsmentioning

confidence: 99%

Sertraline as a new potential anthelmintic against Haemonchus contortus: toxicity, efficacy, and biotransformation

Zajíčková

Prchal

Navrátilová

et al. 2021

Vet Res

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“…Currently, ferns are not incorporated into the diet of European countries. However, the publication of nutritional properties of plant matrices of ferns may be an incentive to incorporate ferns in European diets ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

“…Ferns are susceptible to little environmental changes, and this taxon is positioning itself as an important ecological indicator, as reported in an ecological indicator study in Mexico ( 13 ). In Europe, one of the most important families of ferns is the Aspleniaceae family ( 14 ), particularly at the Mediterranean coast characterized by its moderated temperatures and high humidity ( 15 ). The phenotype studies classified as Aspleniaceae family (from Greek a , meaning “without” and splen , derivate to “spleen” in reference to its use to treat ailments of that organ), known as “spleenwort,” in the eupolypods II group ( 16 , 17 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd.

et al. 2022

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The forest ecosystem is a source of material resources used since ancient times by mankind. Ferns are part of different oriental systems of traditional medicine due to the phytochemical variety of their fronds, which have allowed their traditional use to be validated through ethnopharmacological studies. In Europe, different cultures have used the same fern with a wide variety of applications due to its presence in most European forests. In recent years, studies on the phytocharacterization and biological activity of the fronds of the main European ferns have been published. In this study, the presence of polyphenolic phytochemicals has been evaluated by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in the fronds of two wild ferns together with in vitro activities in non-tumoral and human tumoral cell lines. The polyphenols were extracted from Asplenium trichomanes L. and Ceterach officinarum Willd. by cold maceration using methanol. The main phytochemicals of polyphenolic origin in the extracts of A. trichomanes and C. officinarum determined by HPLC-MS/MS were the flavonol hyperoside and the phenolic acid chlorogenic acid, respectively. This different polyphenolic nature of both extracts contributes to the divergence of the behavior experienced in the biological activities tested, but none of the extracts showed a cytotoxic or phototoxic profile in the different tested cell lines. However, the cytoprotective values in front of the H2O2 oxidative stress induced in the 3T3 and HaCaT cell lines position these extracts as possible candidates for future health applications.

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“…On the other hand, investigations of ferns in recent years have put forward the idea of these species as a potential source of bioactive compounds with food interest. In certain Eastern cultures of the European continent fronds of ferns are used as a food source ( Langhansova et al, 2021 ). The young fronds of ferns (named fiddleheads), which generally exhibit a higher total phenol content than the corresponding mature fronds, are a source of nutrients and phytochemicals with a high potential to reduce oxidative stress of diseases associated with ageing ( Dvorakova et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Polypodium vulgare L. (Polypodiaceae) as a Source of Bioactive Compounds: Polyphenolic Profile, Cytotoxicity and Cytoprotective Properties in Different Cell Lines

et al. 2021

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Pteridophytes, represented by ferns and allies, are an important phytogenetic bridge between lower and higher plants. Ferns have evolved independently of any other species in the plant kingdom being its secondary metabolism a reservoir of phytochemicals characteristic of this taxon. The study of the potential uses of Polypodium vulgare L. (Polypodiaceae) as medicinal plant has increased in recent years particularly when in 2008 the European Medicines Agency published a monograph about the rhizome of this species. Our objective is to provide scientific knowledge on the polar constituents extracted from the fronds of P. vulgare, one of the main ferns of European distribution, to contribute to the validation of certain traditional uses. Specifically, we have characterized the methanolic extract of P. vulgare fronds (PVM) by HPLC-DAD and investigated its potential cytotoxicity, phototoxicity, ROS production and protective effects against oxidative stress by using in vitro methods. The 3T3, HaCaT, HeLa, HepG2, MCF-7 and A549 were the cell lines used to evaluate the possible cytotoxic behaviour of the PVM. HPLC-DAD was utilized to validate the polyphenolic profile of the extract. H2O2 and UVA were the prooxidant agents to induce oxidative stress by different conditions in 3T3 and HaCaT cell lines. Antioxidant activity of in vitro PVM in 3T3 and HaCaT cell lines was evaluated by ROS assay. Our results demonstrate that PVM contains significant amounts of shikimic acid together with caffeoylquinic acid derivatives and flavonoids such as epicatechin and catechin; PVM is not cytotoxic at physiological concentrations against the different cell lines, showing cytoprotective and cellular repair activity in 3T3 fibroblast cells. This biological activity could be attributed to the high content of polyphenolic compounds. The fronds of the P. vulgare are a source of polyphenolic compounds, which can be responsible for certain traditional uses like wound healing properties. In the present work, fronds of the common polypody are positioned as a candidate for pharmaceutical applications based on traditional medicine uses but also as potential food ingredients due to lack of toxicity at physiological concentrations.

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European ferns as rich sources of antioxidants in the human diet

Cited by 24 publications

References 61 publications

Sertraline as a new potential anthelmintic against Haemonchus contortus: toxicity, efficacy, and biotransformation

Sertraline as a new potential anthelmintic against Haemonchus contortus: toxicity, efficacy, and biotransformation

Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd.

Polypodium vulgare L. (Polypodiaceae) as a Source of Bioactive Compounds: Polyphenolic Profile, Cytotoxicity and Cytoprotective Properties in Different Cell Lines

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