Eurycinoid: Potensi Rumput Gong (Eurycaulon Cinereum) Khas Bangka Belitung Sebagai Kandidat Senyawa Antikanker Payudara Berbasis Bahan Alam

Abstract

Traditionally, some People from bangka belitung has consumed Eriocaulon cinereum R.Br as an anticancer drug. Aims of this study is to determine the potential of cytotoxicity of Fraction of Ethyl Acetate and Dichlorometane against T47D cell. Fractionation using Vacuum Liquid Chromatography (VLC) method. MTT assay method was used on evaluating the citotoxic activity. The fraction of dichloromethane performed high activity as a chemopreventive agent with IC50 score was 131,921 µg/ml to T47D and 413,042 µg/ml cells to vero cell whereas for ethyl acetate fraction performed lower activity as chemopreventive agent with IC50 score was 531,808 µg/ml to T47D and 679,114 µg/ml to vero cell). Phytochemical tests showed that the dichloromethane fraction contains phenolic, steroid and terpenoid compounds. While ethyl acetate fraction contains alkaloids, flavonoids and terpenoids. These compounds have activity on cancer cells through apoptotic mechanisms. The results showed the excellent potential of this plant in killing breast cancer cells.

Anticancer Activity of Subfractions from Eriocaulon cinereum R.Br Extract in Cervical Cancer Cells

“…These results align with previous research on T47D cells, which showed that the subfraction containing terpenoids had the best activity. 23,24 However, these results do not align with previous studies on MCF-7 cells, which showed that the subfraction with the best activity contained flavonoids. [20][21][22] This discovery shows that more than one type of active compound, both subfractions of flavonoids and terpenoids, is contained in E. cinereum and can be developed as an anticancer compound.…”

“…These results follow previous studies of MCF-7 or T47D cells, in line with the hypothesis proposed in this study. [20][21][22][23][24][25][26] The compound selectivity index, which was also determined in this study, showed that the selectivity of compounds in normal Vero cells was 2.157, which is classified as being in a good category with a value >2. This shows that the compound is selective and can differentiate between cancer cells and normal cells.…”

“…The subfraction containing terpenoids had a lower IC50 compared to the dichloromethane fraction, with IC50 values of 84.8 and 131.921 μg/mL, respectively. 23,24 However, research using this species on HeLa cervical cancer cells has only been done at the fraction level, with the best IC50 being in the ethyl acetate fraction with an IC50 of 235.65 μg/mL. 25,26 Based on previous research, we hypothesized that the subfraction and isolates from this fraction would have better activity than the crude extract on HeLa…”