Euzebya pacifica sp. nov., a novel member of the class Nitriliruptoria

Abstract

A Gram-stain-positive, aerobic, chemo-organotrophic, rod-shaped, non-spore-forming strain, which produced convex, circular, pink-pigmented colonies, designated as DY32-46T, was isolated from seawater collected from the Pacific Ocean. DY32-46T was found to grow at 20–40 °C (optimum, 30–35 °C), pH 6.0–8.0 (optimum, pH 6.5) and with 0–5 % (w/v) NaCl (optimum, 1–2 %). The results of chemotaxonomic analysis indicated that the respiratory quinone of DY32-46T was MK-9(H4), and major fatty acids (>10 %) were C17 : 1 ω8c, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and C15 : 1 ω6c. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified glycolipids, three unidentified phospholipids, one unidentified phosphoglycolipid and five unidentified lipids. The DNA G+C content of DY32-46T was 70.6 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences and genomic data indicated that DY32-46T should be assigned to the genus Euzebya . ANI and in silico DNA–DNA hybridization values between strain DY32-46T and type strains of Euzebya species were 73.1–87.2 % and 20.2–32.4 %, respectively. Different phenotypic properties, together with genetic distinctiveness, demonstrated that strain DY32-46T was clearly distinct from recognized species of the genus Euzebya . Therefore, DY32-46T represents a novel species within the genus Euzebya , for which the name Euzebya pacifica sp. nov is proposed. The type strain is DY32-46T (=MCCC 1K03476T=KCTC 49091T).

Salsipaludibacter albus gen. nov., sp. nov., a novel actinobacterial strain isolate from a Portuguese solar saltern and proposal of Salsipaludibacteraceae fam. nov. and Salsipaludibacterales ord. nov.

“…S7). Accordingly, the predominant menaquinone in the closest related strains, Egicoccus halophilus [9], Egibacter rhizosphaerae [10], Euzebya rosea [60], Euzebya tangerina [8] and Euzebya pacifica [61] was also MK-9(H 4 ). Although no previous information about the respiratory quinones of N. alkaliphilus ANL-iso2 T was reported, in the present work, MK-9(H 4 ) was also identified as the major menaquinone in this strain (72.2%) by comparison with the menaquinone HPLC profile of strain AS10 T (Fig.…”

“…Like its closely related strains, AS10 T presented an A1ϒ-type cell-wall peptidoglycan, but instead of the meso -diaminopimelic acid found in Egicoccus halophilus [9], Egibacter rhizosphaerae [10], Euzebya tangerina [8], Euzebya rosea [60] Euzebya pacifica [61] and N. alkaliphilus [7], AS10 T presented 3-hydroxy-diaminopimelic acid, which is common for certain sporoactinomycetes, as members of the family Micromonosporaceae [62].…”

“…A set of seven different unidentified phospholipids (PLs) were detected for strain AS10 T and no aminolipids or glycolipids were found (). Studies on the closest related strains Egicoccus halophilus [9], Egibacter rhizosphaerae [10], Euzebya tangerina [8], Euzebya rosea [60] and Euzebya pacifica [61] also detected different types of phospholipids, moreover unidentified glycolipids were further detected for Euzebya tangerina and for Euzebya pacifica . This last species also had other unidentified lipids.…”

“…S7). Accordingly, the predominant menaquinone in the closest related strains, Egicoccus halophilus [9], Egibacter rhizosphaerae [10], Euzebya rosea [60], Euzebya tangerina [8] and Euzebya pacifica [61] was also MK-9(H 4 ). Although no previous information about the respiratory quinones of N.…”

“…[64], sequence identities lower than 94.5% highlight the occurrence of divergent genus. All other members of the class Nitriliruptoria showed low sequence similarity to AS10 T , namely Egicoccus halophilus [9] with 88.91%, Euzebya rosea [60] with 87.30%, Euzebya pacifica [61] with 87.15%, Egibacter rhizosphaerae [10] with 87.08% and Euzebya tangerina [8] with 86.76%. Strain AS10 T clustered together with N.…”