Evagination of the amniotic cavity in larvae derived from lithium-treated embryos of a direct developing echinoid,Peronella japonica

Kitazawa, Chisato; Amemiya, Shonan

doi:10.1002/(sici)1097-010x(19971015)279:3<309::aid-jez12>3.0.co;2-4

Cited by 8 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ratio of LiCl‐treated larvae with an evaginated amniotic cavity appeared to increase proportionally to the concentration of LiCl (Table 2). However, a remarkable variation in LiCl sensitivity of embryos was found among individuals from different batches or even individuals derived from the same batch, as suggested in our previous paper (Kitazawa & Amemiya 1997). The stage of the embryos most sensitive to induction of evagination of the amniotic cavity by LiCl was the period from 2 to 3 h (8‐ to 32‐cell stage) after fertilization, which is earlier than the period in which LiCl can induce exogastrulation.…”

Section: Resultssupporting

confidence: 78%

Regulating potential in development of a direct developing echinoid, Peronella japonica

Kitazawa

Amemiya

2001

Dev Growth Differ

Self Cite

View full text Add to dashboard Cite

The regulating potential along the animal-vegetal axis of a direct developing echinoid, Peronella japonica, was investigated using LiCl. Animal caps isolated from 16-cell stage P. japonica embryos developed to permanent blastulae with an amniotic cavity. Treatment of animal caps with LiCl induced them to vegetalize with differentiation of the endoderm and subsequently develop into pluteus-like larvae. The larvae derived from the LiCl-treated animal caps were able to metamorphose and establish an adult body plan. A considerable fraction of whole embryos treated with LiCl exogastrulated and/or evaginated an amniotic cavity. The timing of the sensitivity to LiCl-mediated induction of evagination of the amniotic cavity was earlier than that for exogastrulation. Peronella japonica embryos became sensitive to LiCl induction of exogastrulation later than embryos of indirect developers. Some larvae with evaginated archenteron and/or evaginated amniotic cavity had metamorphic potential. These results suggest that LiCl can induce both vegetalization and evagination of invaginating structures. The present study is the first to show the potential of the presumptive ectoderm region to regulate the establishment of the adult body plan without any influence from other blastomeres, revealing that the regulating potential of sea urchin embryos is much larger than previously thought.

show abstract

Section: Resultssupporting

confidence: 78%

Regulating potential in development of a direct developing echinoid, Peronella japonica

Kitazawa

Amemiya

2001

Dev Growth Differ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many researchers reported that anomalies could be induced after exposure to heavy metals. It may lead to correlation of the cause with some specific metal if it can be shown that certain metals cause specific anomalies with unique characteristics, such as an abnormal shape of the embryos [13,14].…”

Section: Introduction / Uvodmentioning

confidence: 99%

Electrolites and Heavy Metals in Coelomic Fluid of Sea Urchin, Arbacia lixula from Adriatic Sea: Biochemical Approach to Ecotoxicological Study

2019

View full text Add to dashboard Cite

The main circulatory medium of echinoderms is the coelomic fluid. Biochemistry of coelomic fluid is very complex and slightly different from seawater. The main aim of this research was to analyse concentration of electrolytes (potassium, calcium, magnesium, chlorine and sodium), heavy metals (lead, chromium, cadmium and cobalt) and iron in coelomic fluid of sea urchin, Arbacia lixula as an indirect indicator of seawater contamination. After precise statistical evaluation it was observed that electrolyte concentrations were; Na 189.20±11.41 mmol/l, Cl 134.06±37.08 mmol/l, Mg 4.24±1.08 mmol/l, Ca 3.04±0.84 mmol/l. Biochemical content of heavy metals in coelomic fluid was; Co 1.292±0.879 ppm, Pb 0.644±0.131 ppm, Cr 0.116±0.055 ppm, Cd 0.031±0.017 ppm and iron 0.259±0.058 ppm, and it depends on the potential accumulation level in the organism. The composition of electrolyte and heavy metal content varies depending on the composition of seawater. Obtained values are within the range of values most commonly found in low polluted areas of the Adriatic sea. Similar models may be applied for monitoring of heavy metal contamination in marine environment. Na 189.20±11.41 mmol/l, Cl 134.06±37.08 mmol/l, Mg 4.24±1.08 mmol/l, Ca 3.04±0.84 mmol/l). Biokemijski sadržaj teških metala je iznosio (Co 1.292±0.879 ppm, Pb 0.644±0.131 ppm, Cr 0.116±0.055 ppm, Cd 0.031±0.017 ppm) i željeza 0.259±0.058 ppm, te je ovisio o stupnju akumulacije u Sažetak Glavni cirkulatorni medij bodljokožaca je celomska tekućina. Celomska tekućina ima složen sastav i različita je od sastava morske vode. Glavni cilj ovog istraživanja bio je analiza koncentracije elektrolita (kalija, kalcija, magnezija, klorida i natrija), teških metala (olova, kroma, kadmija i kobalta) i željeza u celomskoj tekućini morskog ježinca Arbacia lixula, kao neizravnih indikatora onečišćenja morske vode. Nakon precizne statističke analize utvrđeno je da su koncentracije elektrolita iznosile ( organizmu. Sastav celomske tekućine u pogledu koncentracije elektrolita i teških metala varira ovisno o sastavu morske vode. Dobiveni rezultati su u razini vrijednosti koje se mogu naći u područjima s niskim stupnjem onečišćenja u Jadranskom moru. Slični modeli mogu se i ubuduće primijeniti u praćenju onečišćenja teških metala u morskom okolišu. KEY WORDS Adriatic seaArbacia lixula ecotoxicology electrolytes heavy metal KLJUČNE RIJEČI Jadransko more Arbacia lixula ekotoksikologija elektroliti teški metal

show abstract

Unusual coelom formation in the direct‐type developing sand dollar Peronella japonica

Tsuchimoto

Yamada

Yamaguchi

2011

Developmental Dynamics

View full text Add to dashboard Cite

Peronella japonica is a sand dollar with a zygote that develops into an abbreviated pluteus but then metamorphoses on day three. The adult rudiment formation is unique; it uses a median position of the hydrocoel and a stomodeum-like invagination of vestibule that covers the dorsal side of the hydrocoel. However, the developmental processes underlying coelom formation remain unclear. In this study, we examined this process by reconstructing three-dimensional images from serial sections of larvae. We show that the left coelom developed by both schizocoely and enterocoely from the archenteron tip, whereas the hydrocoel and right coelom formed by enterocoely from the archenteron. This coelom formation arranged the coelomic compartments directly along the adult oral-aboral axis by skipping the initial bilateral phases. Furthermore, our data indicate P. japonica retains ancestral asymmetry along the left-right axis in the location of the adult rudiment.

show abstract

Evagination of the amniotic cavity in larvae derived from lithium-treated embryos of a direct developing echinoid,Peronella japonica

Cited by 8 publications

References 11 publications

Regulating potential in development of a direct developing echinoid, Peronella japonica

Regulating potential in development of a direct developing echinoid, Peronella japonica

Electrolites and Heavy Metals in Coelomic Fluid of Sea Urchin, Arbacia lixula from Adriatic Sea: Biochemical Approach to Ecotoxicological Study

Unusual coelom formation in the direct‐type developing sand dollar Peronella japonica

Contact Info

Product

Resources

About