Evaluación de la tinción de Hucker para la búsqueda rutinaria de Campylobacter sp en el estudio de un síndrome diarreico agudo

C., Leonardo Chanqueo; C, Patricia García; C, Eugenia León; F., Antonieta Blu

doi:10.4067/s0716-10182005000300004

Cited by 11 publications

(17 citation statements)

References 23 publications

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“…is one of the most important enteropathogens in the United States, not only due to the number of patients involved, but also by the hospitalizations and deaths reported in this country (Center for Disease Control and Prevention [CDC], 2011). Campylobacter jejuni is also the second most frequently isolated gastrointestinal pathogen in stool samples of humans with acute diarrheal disease in Chile (Chanqueo, Garcı´a, Leo´n, & Blu, 2005).…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuniisolated from human cases in Chile showed indistinguishable pulsed field gel electrophoresis profiles with strains isolated from poultry and bovine sources

Hein¹,

García²,

Foerster³

et al. 2013

CyTA - Journal of Food

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Section: Introductionmentioning

confidence: 99%

Campylobacter jejuniisolated from human cases in Chile showed indistinguishable pulsed field gel electrophoresis profiles with strains isolated from poultry and bovine sources

Hein¹,

García²,

Foerster³

et al. 2013

CyTA - Journal of Food

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“…Todos tuvieron buena evolución clínica siendo dados de alta en promedio a los 4 ± 1 días. Todos los cultivos de deposiciones estudiados en nuestro centro asistencial resultaron positivos para S. Enteritidis, y negativos para Shigella sp, Yersinia sp, utilizando siembras en agar Hektoen, (Biomerieux®, Ha, MO), Mac Conkey (Biomerieux®, Ha, MO) y caldo selenito con resiembra en Hektoen después de incubar durante 16 horas a 35° C, bajo métodos de identifi cación convencionales 6 . El análisis con tinción de Hucker para Campylobacter también fue negativo.…”

Section: Discussionunclassified

Descripción clínica y epidemiológica de un grave brote de salmonelosis transmitida por alimentos

García-Huidobro

Carreño²,

Alcayaga

et al. 2012

Rev. chil. infectol.

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Background: Foodborne diseases have increased considerably. Aim: To report a foodborne outbreak, remarking the importance of early notifi cation to activate the epidemiological surveillance system. Results: During February 2011 we observed a Salmonella Enteritidis outbreak. 31.6% of the cases were seen in the same Emergency Care Unit where all required intravenous fl uid rehydration, and 41.7% were hospitalized because of severe dehydration. In the Emergency Room 45.5% of cases required a second visit to be diagnosed correctly. Discussion: Physicians under report the cases of this disease, delaying the activation of the epidemiological surveillance system. Conclusions: Besides providing good treatment to patients, physicians need to be qualifi ed to recognize foodborne diseases and communicate early the suspicion of an outbreak to the epidemiological surveillance system in order to prevent new cases of disease in the community.

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“…However, these methods are insufficiently evaluated and have drawbacks such as operator dependency. Due to sensitivity problems [14], they might underestimate the true incidence of this organism.…”

Section: Introductionmentioning

confidence: 99%

Impact of changing from staining to culture techniques on detection rates of Campylobacter spp. in routine stool samples in Chile

et al. 2016

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BackgroundCampylobacter is a leading cause of bacterial gastroenteritis, but sensitive diagnostic methods such as culture are expensive and often not available in resource limited settings. Therefore, direct staining techniques have been developed as a practical and economical alternative. We analyzed the impact of replacing Campylobacter staining with culture for routine stool examinations in a private hospital in Chile.MethodsFrom January to April 2014, a total of 750 consecutive stool samples were examined in parallel by Hucker stain and Campylobacter culture. Isolation rates of Campylobacter were determined and the performance of staining was evaluated against culture as the gold standard. Besides, isolation rates of Campylobacter and other enteric pathogens were compared to those of past years.ResultsCampylobacter was isolated by culture in 46 of 750 (6.1 %) stool samples. Direct staining only identified three samples as Campylobacter positive and reached sensitivity and specificity values of 6.5 and 100 %, respectively. In comparison to staining-based detection rates of previous years, we observed a significant increase of Campylobacter cases in our patients.ConclusionDirect staining technique for Campylobacter had a very low sensitivity compared to culture. Staining methods might lead to a high rate of false negative results and an underestimation of the importance of campylobacteriosis. With the inclusion of Campylobacter culture, this pathogen became a leading cause of intestinal infection in our patient population.

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Evaluación de la tinción de Hucker para la búsqueda rutinaria de Campylobacter sp en el estudio de un síndrome diarreico agudo

Cited by 11 publications

References 23 publications

Campylobacter jejuniisolated from human cases in Chile showed indistinguishable pulsed field gel electrophoresis profiles with strains isolated from poultry and bovine sources

Campylobacter jejuniisolated from human cases in Chile showed indistinguishable pulsed field gel electrophoresis profiles with strains isolated from poultry and bovine sources

Descripción clínica y epidemiológica de un grave brote de salmonelosis transmitida por alimentos

Impact of changing from staining to culture techniques on detection rates of Campylobacter spp. in routine stool samples in Chile

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