Evaluación de tres métodos para la inactivación de coliformes y Escherichia coli presentes en agua residual doméstica, empleada para riego

Abstract

<p><strong> </strong><strong>Objective.</strong> To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulations from normal bone marrow, and to evaluate the expression of different lineage-specific cell markers with a reactivity associated to cell differentiation to be used as part of the routine quality control in cytometry laboratories. <strong>Materials and methods. </strong>The immunophenotypical analysis of different cell subpopulations was done with samples from normal bone marrow using a panel of monoclonal and polyclonal antibodies useful in the characterization of acute leukemias with four different fluorescences, by means of a protocol that combines cell labeling of membrane and cytoplasm antigens. Expression analysis was done in terms of mean fluorescence intensity (MFI). Fluorescent beads at a known concentration were added for calculating the absolute count of cells. <strong>Results.</strong> The antibody panel used allowed the identification and quantification of different normal leukocyte subpopulations of lymphatic and myeloid origin, including CD34+ stem cells and more differentiated cell populations in the granulocytic, monocytic, and erythroid cell lines. We established reference values for cell populations and cell marker expression ranges as part of routine quality control of cytometry laboratories. <strong>Conclusion.</strong> Immunophenotypic patterns identified as well as absolute and relative reference values for the different normal leukocyte populations from bone marrow can be used by cytometry laboratories as a basis for establishing reference parameters in phenotypic analyses of hematologic neoplasia.<strong> </strong></p> <p><strong>Key words: </strong>multiparametric flow cytometry, immunophenotype, hematologic neoplasia, normal bone marrow, reference values, quality control.</p><br />

Physicochemical and Biological Structure as Indicators of the Environmental Quality of Surface Water Streams

“…This classification is supported by the presence of families such as Perlidae (Plecoptera), Leptohyphidae (Ephemeroptera), and Hydrobiosidae (Trichoptera), which scored 10.7 and 9, respectively, in the BMWP/Col index (Roldán-Pérez, 2003). These findings are consistent with the biological characteristics of these macroinvertebrate families, indicating their presence in specific or more restricted ranges (Allan et al, 2006;Gamboa et al, 2008;Pérez et al, 2021;Rojas-Higuera et al, 2010).…”

“…This was facilitated by the configuration of the channel, characterized by the presence of large stones and rocks at the sampling point, allowing for efficient aeration of the system. The low concentrations of BOD (<4.0 mg/L) further highlight the impact of organic component on the water quality, in line with the findings of Rizzo (2009) and Rojas-Higuera, et al (2010).…”

Detoxification of pulping black liquor with Pleurotus ostreatus or recombinant Pichia pastoris followed by CuO/TiO2/visible photocatalysis

“…In addition, to verify yeast viability, decimal dilutions and microdrop cultures were performed on YPG agar, following a previously reported methodology 70 . The SBL concentration to be used in the removal curves was selected in the same manner as for P. ostreatus .…”

Potencial de plantas acuáticas para la remoción de coliformes totales y Escherichia coli en aguas negras

“…Dentro de los tratamientos que se han propuesto para saneamiento de las aguas residuales se encuentran la cloración, el tratamiento de lagunaje facultativo y la fotocatálisis heterogénea. La eficiencia de remoción de coliformes de los tratamientos tradiciones se encuentran en rangos de 55,6% con cloración, 90% con tratamiento de lagunaje facultativo y 100% utilizando fotocatálisis heterogénea con TiO2 (Rojas-Higuera et al, 2010). Sin embargo, estos tratamientos son costosos y poco asequibles para la población rural.…”