Introduction: Periodontal disease and dental caries are the two most common dental diseases. Childhood is an essential period to adopt skills and habits of healthy life. So, education for health constitutes a set of important actions in the dental hygiene of children. Our objective is to validate the content of a questionnaire to evaluate oral health habits and practices in 5th and 6th grade primary school students. Material and method: A bibliographic search was made in the main databases and the questionnaire was elaborated. To validate the content, the initial version of the questionnaire was sent by e-mail to the panel of experts, the quality of each item was evaluated (I-CVI), as well as the overall validity of the instrument (S-CVI). In addition, a pilot test was carried out with a reduced sample (10 subjects) with the same characteristics as the sample under study. Results: A questionnaire consisting of three blocks and a global validity of 0.92 was obtained. In the first block, the sociodemographic data were valued, and, in the second, consisting of 23 items, oral health habits and practices were valued. In the third, the O'Leary index. The results of the pilot test showed that the questionnaire was understood by the target population. Conclusion: The questionnaire presented adequate content validity results, so it is possible to use it for future research.